 ผลงานตีพิมพ์ในวารสารวิชาการCatfish species identification using lab-on-chip PCR-RFLPผู้แต่ง: Wong, L.L., Peatman, E., Kelly, L., Kucuktas, H.,  Dr.Uthairat Na-Nakorn, Professor , Liu, Z., วารสาร:  
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 ที่มา:วิทยาศาสตร์เกษตรหัวเรื่อง:การวิเคราะห์สายพันธุ์เห็ดฟางด้วยวิธี ITS-PCR-RFLP |
 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : In silico PCR-RFLP of Bacillus Species: Problem-Based Case of Teaching Bioinformatics) ผู้เขียน:Ekachai Chukeatirote, Komsit Wisitrasamewong, Jedsadapa Jongmahasavat สื่อสิ่งพิมพ์:pdf AbstractBioinformatics is a multi-disciplinary subject that encompasses a wide range of fields including; genomics, biotechnology, information technology, algorithms and statistics. As a result, it is important to establish a skeletal set of courses capable of providing both a general and a detailed background on all aspects of bioinformatics. In this study, a problem-based project entitled “in silico PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) of Bacillus species” was assigned to students. The project aimed to cover the background of genetic information retrieval, multiple sequence alignment, phylogenetic analysis and in silico PCR-RFLP. Initially, the 16S rRNA genes of 34 Bacillus species were retrieved from the NCBI database. The sequence data obtained were then analyzed in terms of their similarities and variation using the ClustalW software. Subsequently, the phylogenetic tree was constructed to investigate their evolutionary relationship. The results indicated that these Bacillus species could be grouped into different clades. In addition, in silico PCR-RFLP was performed to introduce students to the principles of molecular taxonomy. |
ที่มา:การประชุมทางวิชาการของมหาวิทยาลัยเกษตรศาตร์ ครั้งที่ 48หัวเรื่อง:การแยกชนิสัตว์ในสกุล Varanus spp. ที่พบในประเทศไทยดยเทคนิค Polymerase Chain reaction-Restriction Fragment Length Polymorphophism (PCR-RFLP) |
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หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Species Identification of 3 Hypsibarbus spp. (Pisces: Cyprinidae) Using PCR–RFLP of Cytochrome b Gene) ผู้เขียน:ดร.พัฒนี จันทรโรทัย, รองศาสตราจารย์ , สุรภพ สุทธิวิเศษ, Wongpathom Kamonrat, สุรินทร์ ปิยะโชคณากุล, Chavalit Vidthayanon สื่อสิ่งพิมพ์:pdf AbstractPolymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) was used to identify 3 closely related Hypsibarbus spp: Hypsibarbus wetmorei, H. vernayi, and H. malcolmi. Mitochondrial cytochrome b gene (993 bp) in 3 Hypsibarbus spp. showed single PCR– product. The sequencing results of PCR–products in 3 Hypsibarbus spp. showed very low interspecific variation. However it could be used to discriminate these species by RFLP analysis. The combination of 2 restriction enzymes; Bsp143I and BcuI were used to identify 3 Hypsibarbus spp. Bsp143I could discriminate H. vernayi from H. wetmorei and H. malcolmi, by generating 3 fragments (535 bp, 234 bp and 224 bp) in H. vernayi whereas 2 fragments of 769 bp and 224 bp in H. wetmorei and H. malcolmi. Thereafter, BcuI was effectively discriminated H. wetmorei from H. malcolmi by generating 3 fragments (591 bp, 288 bp and 114 bp) in H. malcolmi and uncut fragment in H. wetmorei. There were intraspecific restriction polymorphism in H. vernayi using BcuI which generated 2 patterns; an uncut fragment and 2 fragment of approximately 700 bp and 300 bp. Thus, PCR–RFLP technique could be used to identify 3 closely related Hypsibarbus spp. |
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