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ผลงานตีพิมพ์ในวารสารวิชาการGrowth modulation effects of CBM2a under the control of AtEXP4 and CaMV35S promoters in Arabidopsis thaliana, Nicotiana tabacum and Eucalyptus camaldulensisผู้แต่ง:Pornthep Keadtidumrongkul, Dr.Anongpat Suttangkakul, Assistant Professor, Phitsanu Pinmanee, Kanokwan Pattana, Chokchai Kittiwongwattana, Somsak Apisitwanich, Dr.Supachai Vuttipongchaikij, Associate Professor, วารสาร: |
ผลงานตีพิมพ์ในวารสารวิชาการComplex nested promoters control tissue-specific expression of acetyl-CoA carboxylase genes in wheatผู้แต่ง:Zuther E, Huang, S, Jelenska, J, Eilenberg, H., Arnold, E.M., Su, X., Dr.Anchalee Sirikhachornkit, Associate Professor, Podkowinski, J., Zilberstein, A., Haselkorn, R., Gornicki, P., วารสาร: |
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ผลงานตีพิมพ์ในวารสารวิชาการEffects of Potassium and Manganese Promoters on Nitrogen-Doped Carbon Nanotube-Supported Iron Catalysts for CO2 Hydrogenationผู้แต่ง:Dr.Praewpilin Kangvansura, Ly MayChew, Dr.Chanapa Kongmark, Associate Professor, Phatchada Santawaja, Holger Ruland, Wei Xia, Hans Schulz, Dr.Attera Worayingyong, Associate Professor, Martin Muhler, วารสาร: |
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หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Evaluation of Constitutive Promoters for Gene Expression in Dendrobium Protocorms and Flowers) ผู้เขียน:Chidchanok Suwannaketchanatit, พรสุข ชัยสุข, Jitraparn Piluek, ดร.สุรินทร์ ปิยะโชคณากุล, รองศาสตราจารย์, พัฒนา ศรีฟ้า ฮุนเนอร์ สื่อสิ่งพิมพ์:pdf AbstractActively dividing protocorms of Dendrobium Jaquelyn Thomas orchid were produced on VW medium and transferred to 1/2 MS medium before subjecting to particle bombardment to evaluate the promoter efficiency. Three different constitutive promoters, rice Act-1, CaMV35S, and maize Ubi-1, were determined for the efficiency to control spatial transgene expression in protocorms and sepal tissues of Dendrobium orchid. Each promoter was fused to b-glucuronidase (uid A) gene and nos terminator in the plant expression vectors including pActin1-D, pAHC27, and p2K7. The plasmid vectors were individually bombarded into protocorms and fresh sepals of Dendrobium. Transient gus gene expression was assayed after 3 days of bombardment. The results demonstrated that all tested promoters could drive gus gene expression in the orchid protocorms at different levels. Ubi-1 promoter conferred the most efficient promoter in protocorms giving the highest level of gus gene expression, whereas GUS activity was distinctly observed under the control of Act-1 and CaMV35S in the Dendrobium sepal tissues. Both Act-1 and CaMV35S promoters performed well in the vascular tissue of flower as opposed to the Ubi-1 promoter that did not perform in flower. |