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การประชุมวิชาการEffects of culture media and plant growth regulators on callus induction of Curcuma longa L.ผู้แต่ง: Dr.Piyamat Srirat, Lecturer , Dr.Sarote Sirisansaneeyakul, Professor , Dr.Pramuk Parakulsuksatid, Associate Professor , Dr.Siripatr Prammanee, Associate Professor , Dr.Wirat Vanichsriratana, Associate Professor , การประชุมวิชาการ:  
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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Callus induction and Growth of Zea mays. L var. saccharata (Super sweet corn) through Tissue Culture ) ผู้เขียน: Tanong Pronpradupkait, Paiboolya Gavinlertvatana สื่อสิ่งพิมพ์:pdf AbstractCallus induction of Zea mays var. saccharata (super sweet) on Murashige and Skoog (1962) medium containing 1 or 3 mg/1 2, 4-D was found. Tissue explants from root tip and mesocotyl were suitable for callus formation. The induction and growth of callus was better in medium containing 1 mg/1 2, 4-D and 10% coconut milk than medium containing 3 or mg/1 2, 4-D and coconut milk. Callus from root tip which was cultured in medium containing 1 or mg/1 2, 4-D grew at a greater extent under condition of light than in the dark. In contrast, callus from mesocotyl which was cultured in medium containing 1 mg/1 2, 4-D grew better under condition of dark than in the light. Nevertheless, callus grew better in the dark in medium which was supplemented with %coconut milk. The increasing percentage of callus growth was greater in medium containing 3 mg/1 2,4-D and 10% coconut milk or in coconut milk free-medium. Root formation occurred in callus which was cultured in medium containing 1 or 3 mg/1 2,4-D than in medium containing 1 mg/1 2,4-D than in medium containing 3 mg/1 2,4-D. |
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หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Callus Induction and Somatic Embryogenesis from Anther Cultures of Hevea brasiliensis Muell Arg.) ผู้เขียน:Wutthichai Srichuay, Soontreeya Kalawong, Yupaporn Sirisom, Sompong Te-chato สื่อสิ่งพิมพ์:pdf AbstractHevea brasiliensis is an economically important perennial tree in southeast Asia. However, few studies have been conducted to evaluate the effects of sucrose and genotype on callus induction and plantlet regeneration from anther culture. Male flowers at the uninucleate stage were collected from clones numbered 2-nr, 6-nr, 1-tF and 1-em. An excised cluster of anthers was cultured on callus induction medium (CIM) consisting of Murashige and Skoog (MS) medium supplemented with 1 mg.L-1 2,4-diclorophenoxyacetic acid, 1 mg.L-1 kinetin (KN), 1 mg.L-1 ?-naphthalene acetic acid (NAA) and sucrose at various concentrations ranging from 3 to 10%. The results revealed that the highest callus induction frequency (87.5%) was obtained from clone 1-em on CIM with 8% sucrose. Callus derived from clone 2-nr culturing on CIM supplemented with 5% sucrose gave the highest frequency and number of somatic embryo (SEm) formations. CIM supplemented with 5% sucrose and 5% coconut water caused a decrease in the percentage of callus induction (30.88%). The highest SEm formation at 20% was obtained when callus of clone 2-nr aged 8 wk was cultured on MS supplemented with 3% sucrose, 0.2 mg.L-1 NAA, 1 mg.L-1 6-benzyladenine, 3 mg.L-1 KN and 0.05 mg.L-1 gibberellic acid (GA3). When calluses were cultured on CIM supplemented with 5% sucrose and maintained in darkness, 16% SEm formation was obtained. Calluses and SEms from the culture medium showed the same ploidy level as the mother plant after evaluation by flow cytometry. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Callus Induction and Plant Regeneration from Mature Embryos of Glutinous Rice (Oryza sativa L.) Cultivar TDK1) ผู้เขียน:Souvanh THADAVONG, ดร.ประภา ศรีพิจิตต์, รองศาสตราจารย์ , ดร.วาสนา วงษ์ใหญ่, รองศาสตราจารย์ , ดร.พีรนุช จอมพุก, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractThe present study was conducted to determine the optimum in vitro conditions for callus induction and plant regeneration from mature embryos (seeds) of glutinous rice cultivar TDK 1. It was revealed that embryos cultured on Murashige and Skoog (MS) agar medium supplemented with 2 mg/l 2,4-D and 500 mg/l L-proline under light condition produced the highest percentage of callus formation (96.91 %). The average size of callus produced was large (6.02 mm). The calli dehydrated by placing in petridishes with covers for 7 days under light condition before transferring onto regeneration medium generated higher frequency of shoot regeneration than the calli cultured on regeneration medium without dehydration. The most suitable medium for plant regeneration from dehydrated calli was MS agar medium supplemented with 1 mg/l IAA, 4 mg/l BA and 800 mg/l casein hydrolysate which induced the highest percentage of calli forming shoots (45.00 %) and each callus produced the largest number of shoots (average 15 shoots). The shoots were rooted at the highest rate (100 %) when transferred onto MS agar medium supplemented with 1 mg/l NAA. The complete plantlets were thereafter transplanted to grow under greenhouse condition. They were morphologically normal and fertile. |
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