Search Result of "invertase"

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ผลงานตีพิมพ์ในวารสารวิชาการ

Effects of Inhibitors on Kinetic Properties of Invertase from Saccharomyces cerevisiae

ผู้แต่ง:ImgSirisatesuwon, C., ImgDr.Boontiwa Ninchan, Lecturer, ImgDr.Klanarong Sriroth, Associate Professor,

วารสาร:

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การประชุมวิชาการ

Effects of sugars in vase solution on sugar metabolism and invertase activity in Dendrobium flowers

ผู้แต่ง:

การประชุมวิชาการ:

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การประชุมวิชาการ

Diversity of yeasts isolated from fragrant flowers and their invertase activity

ผู้แต่ง:ImgMs.Chommanat Kerdkhong, ImgDr.Manee Tantirungkij, ImgMrs.ORAWAN CHOUNTRAGOON,

การประชุมวิชาการ:

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ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Relationship Between Invertase Enzyme Activities and Sucrose Accumulation in Sugarcane (Saccharum spp.))

ผู้เขียน:ImgBootsarin Tana, Imgดร.สนธิชัย จันทร์เปรม, รองศาสตราจารย์, ImgNongluck Tienseree, Imgดร.สุคันธรส ธาดากิตติสาร

สื่อสิ่งพิมพ์:pdf

Abstract

The sucrose content, and the activities of sugar metabolism enzymes—namely, soluble acid invertase (SAI), neutral invertase (NI) and cell wall invertase (CWI), of various developmental stages of the stem and the relationships among them were investigated in cultivated sugarcane (Saccharum officinarum L.), line 20-2248 and wild sugarcane S. spontaneum, line S98-244. Stems of both sugarcane species 6–12 mth after planting (MAP) stems were collected and examined with samples consisting of three parts of the internode—the top, middle and bottom—representing relatively immature, maturing and mature internodes, respectively. The results showed that sucrose was the main sugar in the two species. Sugarcane stems of the two species revealed different patterns of sucrose accumulation and activities of invertase (SAI, CWI and NI). The sucrose content of line 20-2248 increased from 6 to 12 MAP and was highest in the bottom part and decreased in the middle and top parts, respectively, whereas the sucrose content of line S98-244 decreased from 6 to 12 MAP and was highest in the middle part than in the lower the bottom and top parts. The activities of SAI, CWI and NI in both sugarcane species were higher in the top part at each growth stage, lower in middle and lowest in the bottom part of the stem. These activities decreased from 6 to 12 MAP with line 20-2248, whereas the activities increased with increasing time after planting for line S98-244. There was a significantly negative correlation between the sucrose content and the activity of SAI, CWI and NI for line 20-2248. With line S98-244, the lowest concentration of sucrose was significantly negative only with SAI activity. The sucrose content correlation with CWI and with NI activity showed negative correlations but they were not significant.

Article Info
Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 048, Issue 6, Nov 14 - Dec 14, Page 869 - 879 |  PDF |  Page 

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ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Optimization of Inulinase, Invertase and ?-fructofuranosidase Production from Aspergillus niger TISTR 3570 by the Taguchi Method)

ผู้เขียน:Imgดร.สาโรจน์ ศิริศันสนียกุล, รองศาสตราจารย์, Imgมลนพรรษ สงพิมพ์, Imgดร.พิลาณี ไวถนอมสัตย์

สื่อสิ่งพิมพ์:pdf

Abstract

The medium conditions for inulinase, invertase and ?-fructofuranosidase (bFFase) production from Aspergillus niger TISTR 3570 were optimized by the Taguchi method. Production of inulinase was most affected by inulin and the production of invertase and bFFase was most affected by pH. The maximum production of enzymes was achieved by employing a medium containing: inulin (50 g.L-1), yeast extract (12 g.L-1), MgSO4?7H2O (6 g.L-1) and an initial pH of 6. The inulinase was thermostable over a wide temperature range (25–80 ?C) and showed maximum activity at 70 ?C. In addition, activity was stable over the range pH 4–10 and the optimum for enzyme activity was pH 5. In contrast, invertase and bFFase were stable below 55 and 50 ?C, respectively, but showed highest activity at 65 and 60 ?C, respectively. Both enzymes were stable over the range pH 4–9 and had optimum activity at pH 4 for invertase and pH 5 for bFFase.

Article Info
Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 046, Issue 2, Mar 12 - Apr 12, Page 238 - 255 |  PDF |  Page 

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ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : A Statistical Approach for Culture Condition Improvement of Invertase and Inulinase from Candida guilliermondii TISTR 5844)

ผู้เขียน:Imgดร.พิลาณี ไวถนอมสัตย์, Imgมลนพรรษ สงพิมพ์, Imgดร.สาโรจน์ ศิริศันสนียกุล, รองศาสตราจารย์

สื่อสิ่งพิมพ์:pdf

Abstract

A newly isolated yeast Candida guilliermondii TISTR 5844 was shown to be a good producer of invertase and inulinase in an inexpensive, 48-hour batch fermentation. The enzyme production was performed using an orthogonal array experimental design and software for design and analysis of the experiment, based on the Taguchi method. The optimized medium for producing invertase consisted of 5% inulin, 4.8% NH4Cl and 0.6% MgSO4?7H2O at pH 6. Maximum activity and productivity were achieved at 552.12 U.L-1 and 10.28 U.L-1.hr-1, respectively. Inulinase production was maximized using a medium of 1% inulin, 2.4% NH4Cl and 1.2% MgSO4?7H2O at pH 5. Under optimum conditions, inulinase activity and productivity were achieved at 56.41 U.L-1 and 1.18 U.L-1.hr-1, respectively. The optimal temperature was 30 ?C for both enzymes.

Article Info
Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 045, Issue 6, Nov 11 - Dec 11, Page 1083 - 1093 |  PDF |  Page 

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ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Enhancement of Inulinase and Invertase Production from a Newly Isolated Candida guilliermondii TISTR 5844)

ผู้เขียน:Imgมลนพรรษ สงพิมพ์, Imgดร.พิลาณี ไวถนอมสัตย์, Imgดร.วิรัตน์ วาณิชย์ศรีรัตนา, รองศาสตราจารย์, Imgดร.สาโรจน์ ศิริศันสนียกุล, รองศาสตราจารย์

สื่อสิ่งพิมพ์:pdf

Abstract

The optimization of inulinase and invertase production from Candida guilliermondii TISTR 5844 was studied using a central composite design with four factors: pH, temperature, inoculum and substrate concentrations. The most significant factors influencing invertase production were pH and temperature with an R2 value of 0.822 (P < 0.05). Unexpectedly, the R2 value for inulinase production was 0.555 (P > 0.05) and there was no significant influence of the tested factors over the production of inulinase. The results obtained from the experimental design using one factor at a time demonstrated the optimum carbon and nitrogen sources for both enzymes were inulin and NH4Cl, respectively. Optimization by Taguchi methodology further indicated the optimum medium compositions for the production of inulinase (inulin concentration 1%, NH4Cl concentration 2.4%, Mg2SO4•7H2O concentration 1.2% and pH 5) and invertase (inulin concentration 5%, NH4Cl concentration 4.8%, Mg2SO4•7H2O concentration 1.2% and pH 6).

Article Info
Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 045, Issue 4, Jul 11 - Aug 11, Page 675 - 685 |  PDF |  Page 

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Researcher

ดร. นงลักษณ์ เทียนเสรี, อาจารย์

ที่ทำงาน:ภาควิชาพืชไร่นา คณะเกษตร กำแพงแสน

สาขาที่สนใจ:การเพาะเลี้ยงเนื้อเยื่อพืช, การถ่ายยีน, เครื่องหมายโมเลกุล

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