 Researcherดร. อุทัยวรรณ โกวิทวที, รองศาสตราจารย์ที่ทำงาน:ภาควิชาสัตววิทยา คณะวิทยาศาสตร์ สาขาที่สนใจ:Ecology, Taxonomy, Culture Gloehidia in Modified Media nad Rearing Adult of Freshwater Mussel Freshwater Ecology, Aquaculture Resume |
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ผลงานตีพิมพ์ในวารสารวิชาการCharacterisation of thermostable trypsin and determination of trypsin isozymes from intestine of Nile tilapia (Oreochromis niloticus L.)ผู้แต่ง: Dr.Sasimanas Unajak, Associate Professor ,  Meesawat, P., Paemanee, A., Dr.Nontawith Areechon, Associate Professor , Dr.Arunee Engkagul, Assistant Professor , Dr.Uthaiwan Kovitvadhi, Associate Professor, Kovitvadhi, S., Rungruangsak-Torrissen, K., Dr.Kiattawee Choowongkomon, Associate Professor , วารสาร:  
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ผลงานตีพิมพ์ในวารสารวิชาการDevelopment of digestive enzymes and in vitro digestibility of different species of phytoplankton for culture of early juveniles of the freshwater pearl mussel, Hyriopsis (Hyriopsis) bialatus Simpson, 1900ผู้แต่ง:Areekijseree, M, Dr.Arunee Engkagul, Assistant Professor, Kovitvadhi, S, Dr.Uthaiwan Kovitvadhi, Associate Professor, Mrs.Amara Thongpan, Associate Professor, Rungruangsak-Torrissen, K, วารสาร: |
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ผลงานตีพิมพ์ในวารสารวิชาการEffects of different modified diets on growth, digestive enzyme activities and muscle compositions in juvenile Siamese fighting fish (Betta splendens Regan, 1910)ผู้แต่ง:Thongprajukaew, K., Dr.Uthaiwan Kovitvadhi, Associate Professor, Kovitvadhi, S., Somsueb, P., Rungruangsak-Torrissen, K., วารสาร: |
ผลงานตีพิมพ์ในวารสารวิชาการDigestive enzymes and in-vitro digestibility of different species of phytoplankton for culture of the freshwater pearl mussel, Hyriopsis (Hyriopsis) bialatusผู้แต่ง:Supannapong, P, Pimsalee, T, A-Komol, T, Dr.Arunee Engkagul, Assistant Professor, Dr.Uthaiwan Kovitvadhi, Associate Professor, Kovitvadhi, S, Rungruangsak-Torrissen, K, วารสาร: |
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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Characterization and Expression Levels of Protease Enzymes at Different Developmental Stages of Siamese Fighting Fish (Betta splendens Regan, 1910)) ผู้เขียน:Karun Thongprajukaew, ดร.อุทัยวรรณ โกวิทวที, รองศาสตราจารย์, ดร.อรุณี อิงคากุล, ผู้ช่วยศาสตราจารย์, Krisna Rungruangsak-Torrissen สื่อสิ่งพิมพ์:pdf AbstractCharacterization of total proteases, trypsin and chymotrypsin were performed at pH 2 12 and temperatures 20-80?C in three life stages of Siamese fighting fish (Betta splendens Regan, 1910). At least fourteen protease activities were detected, including seven acidic (pH levels 2, 4 and 5-6), one neutral (pH 7), and six alkaline (pH levels 8-12) activities in the optimal temperature range of 30-60?C. The neutral and alkaline pH profiles were different between sexes during the on-growing stage (1.5 month-old). The acidic proteases played a major role in digestion in the early life stage (10-day-old), while the alkaline proteases became more important toward older stages. Trypsin and chymotrypsin showed similar characteristics with at least five activities observed. For trypsin, activity was detected with pH 7 and 8 at 50?C, and pH 10 at 30, 50 and 60?C. For chymotrypsin, activity was found at pH levels 7 and 8 at 50?C, and pH 10 at 30, 40 and 50?C. Regardless of sex and age, the most suitable conditions for studying digestive enzyme activities in general were at pH 8 and 50?C for total proteases, pH 7-8 and 50?C or pH 10 and 30-35?C for trypsin and pH 7-8 and 50?C or pH 10 and 40?C for chymotrypsin. Trypsin and chymotrypsin are important proteases showing similar general optimal conditions to that of total proteases. This information provides elementary knowledge for studying protein digestibility by in vitro techniques and for determining growth performance quality using digestive enzyme markers in Siamese fighting fish. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Temperature and pH Characteristics of Amylase and Lipase at Different Developmental Stages of Siamese Fighting Fish (Betta splendens Regan, 1910)) ผู้เขียน:Karun Thongprajukaew, ดร.อุทัยวรรณ โกวิทวที, รองศาสตราจารย์, ดร.อรุณี อิงคากุล, ผู้ช่วยศาสตราจารย์, Krisna Rungruangsak-Torrissen สื่อสิ่งพิมพ์:pdf AbstractTemperature (20-80?C) and pH (2-12) profiles of amylase and lipase at three stages (10-dayold, 1.5-month-old and 3-month-old) in Siamese fighting fish (Betta splendens Regan, 1910) were studied. At least nine amylase activities were observed during development: pH 7 at 40?C, pH 9 at 30?C and 50? C, pH 8 and pH 11 at 50?C in females and pH 8 and pH 11 at 40?C and 60?C in males. At the younger stage (? 1.5 month old), activity at pH 7 and 40?C, and at pH 9 and 30?C and 50?C was observed. At the older stage (? 1.5 month old), amylase activity at pH 8 and pH 11 in the temperature range of 40-60?C was found. Lipase had at least five levels of activity: pH 7 at 20?C and 40?C, pH 8 at 20-40?C and 60?C, and at pH 11, where the activity peak disappeared in the maturing stage (3 month old) in both sexes. Amylase had very low specific activity at the 10-day-old stage, while lipase had a high specific activity, similar to older stages. The most suitable pH to determine amylase and lipase activity in Siamese fighting fish was pH 8, whereas a temperature of 50?C was appropriate for amylase and 40?C was suitable for lipase, regardless of sex and age. This information is a prerequisite for future studies of the in vitro digestibility evaluation of nutrient utilization in Siamese fighting fish. |
ผลงานตีพิมพ์ในวารสารวิชาการEcological Effects on Food Utilization, Trypsin Isozymes, and PerformanceQualities of Growth and Maturation in Northeast Arctic Cod(Gadus morhua L.)ผู้แต่ง:Krisna Rungruangsak-Torrissen, Karun Thongprajukaew, Kanokwan Sansuwan, Passara Thapthimdaeng, Dr.Uthaiwan Kovitvadhi, Associate Professor, Supaporn Seetaha, Kiattawee Choowongkomon, Inger M. Beck, Ole O. Arn?y, วารสาร: 
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