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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Effects of Na+, K+ and Ca2+ Accumulation on the Expression of Ca2+-ATPase Gene in Rice KDML 105) ผู้เขียน: วรรณรดา สุราช,  ดร.มิ่งขวัญ มิ่งเมือง, รองศาสตราจารย์ , นางอมรา ทองปาน, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractHaving been exposed to 150 mM NaCl supplemented in the growth medium, the roots of KDML105 started to accumulate Na+ in three hours but the leaves showed the amount of Na+ change after one day. Addition of either 0.4 mM or 10 mM of CaCl2 to the 150 mM NaCl supplemented medium, on the other hand, caused a small drop in Na+ accumulation in the leaves and roots on day 8. K+, however, significantly decreased in both leaves and roots as the result of NaCl in the medium. As for Ca2+ deposition in KDML105, NaCl supplemented alone in the medium had little effect but the combined NaCl and CaCl2 caused the rise of Ca2+ in both leaves and roots. The expression of Ca2+-ATPase gene in the leaves of KDML105 seemed to be directly activated by the exposure to Na+. The combined effect of Na+ and Ca2+ also showed the drastic change in Ca2+- ATPase expression, and rice, therefore, try to maintain Ca2+ homeostasis by producing more Ca2+- ATPase mRNA especially in the leaves. Since the roots are not the main accumulation site of Na+, the effect of NaCl alone on the Ca2+-ATPase gene in the roots was not as distinctively seen. However, the addition of 10 mM CaCl2 caused a large increase of Ca2+-ATPase gene expression in the roots, while both Na+ and Ca2+ accumulation seemed to affect more to the gene expression in the leaves. Key words: Na+, K+, Ca2+, rice KDML105, Ca2+-ATPase gene, Ca2+-ATPase mRNA |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : DNA Sequences of Ca2+-ATPase Gene in Rice KDML 105) ผู้เขียน:นางสาวศกลวรรณ ประสิทธิ์วิไล, ศรีพรรณ ประเดิมวงศ์, นางอมรา ทองปาน, รองศาสตราจารย์, ดร.มิ่งขวัญ มิ่งเมือง, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractTotal RNA isolated from the leaves of KDML 105 rice was used as template to make complementary DNA (cDNA). Primer combinations of CA1-CA10 which were designed from Lycopersicon esculentum and Arabidopsis thaliana Ca2+-ATPase gene sequence were used. The nucleotide sequences amplified from 7 primer combinations gave 2,480 bp (fragment A). This fragment was found to be 99% homology to that of the putative calcium ATPase of Oryza sativa (Japonica cultivar-group) as shown in the GenBank. Amplification of 3? end fragment done by Rapid Amplification of cDNA Ends (3?RACE) technique using 3?GSP1, 3?GSP2, and 3?UAP as primers gave a DNA fragment of 933 bp having an overlapping region with DNA fragment A, which resulted in the combined length of 2,944 bp (fragment B). To find the sequence of 5? end, 5?RACE technique was used having 5?GSP1, 5?GSP2, 5? AP and 5?UAP as primers. It gave a DNA fragment of 473 bp showing an overlapping region with DNA fragment B, which ultimately resulted in the combined length of 3,331 bp (total CA). The deduced 1,008 amino acid sequence of total CA showed 99% homology to putative calcium ATPase of O. sativa (Japonica cultivar-group) cv. Nipponbare. The higher percent homology of this Ca2+-ATPase gene in KDML105 to that of O. sativa cv. Nipponbare (99%) than to O. sativa cv. IR36 (89%) was not as anticipated since both KDML105 and O. sativa cv. IR36 belong to the same Indica type while O. sativa cv. Nipponbare is a Japonica. Analysis of the amino acid sequences of Ca2+- ATPase gene indicated that this gene in KDML105 most likely belonged to ER type IIA group. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Ca2+ATPase, Carbonic Anhydrase and Alkaline Phosphatase Activities in Red Sternum Mud Crabs (Scylla serrata)) ผู้เขียน:Thanaset Tongsaikling, ดร.จินตนา สและน้อย, รองศาสตราจารย์ , ดร.มิ่งขวัญ มิ่งเมือง, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractRed sternum is a poorly understood symptom affecting mud crab aquaculture in Thailand. This work presents the results of enzymatic activities in mud crab exhibiting the red sternum symptom. The enzymatic activities of Ca2+ATPase, carbonic anhydrase and alkaline phosphatase were analyzed in five tissues (muscle, gill, hepatopancreas, integument and hemolymph) from symptomatic and asymptomatic mud crabs. The main features of the symptom include reddish colored stripes on the abdominal thorax, sternum and chelae, swollen hepatopancreas, milky hemolymph and opaque and whitish muscle. The most distinctive change could be observed in the hemolymph which enabled classification of its severity into three groups—group I: translucent brown hemolymph, group II: cloudy orange hemolymph and group III: milky unclotted hemolymph. Ca2+ATPase levels were highest in the muscle of asymptomatic crabs. This enzyme activity tended to increase in all tissues of symptomatic crabs in groups I and II, especially in the gill of group II where it was fivefold higher than in asymptomatic crabs. Carbonic anhydrase and alkaline phosphatase values were highest in the hepatopancreas of asymptomatic crabs. Alkaline phosphatase decreased in all tissues of the three groups of symptomatic crabs. Carbonic anhydrase levels were highly variable with a decreasing trend in most tissues of groups I and II. |
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