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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Optimization of Inulinase, Invertase and ?-fructofuranosidase Production from Aspergillus niger TISTR 3570 by the Taguchi Method) ผู้เขียน: ดร.สาโรจน์ ศิริศันสนียกุล, ศาสตราจารย์ ,  มลนพรรษ สงพิมพ์, ดร.พิลาณี ไวถนอมสัตย์ สื่อสิ่งพิมพ์:pdf AbstractThe medium conditions for inulinase, invertase and ?-fructofuranosidase (bFFase) production from Aspergillus niger TISTR 3570 were optimized by the Taguchi method. Production of inulinase was most affected by inulin and the production of invertase and bFFase was most affected by pH. The maximum production of enzymes was achieved by employing a medium containing: inulin (50 g.L-1), yeast extract (12 g.L-1), MgSO4?7H2O (6 g.L-1) and an initial pH of 6. The inulinase was thermostable over a wide temperature range (25–80 ?C) and showed maximum activity at 70 ?C. In addition, activity was stable over the range pH 4–10 and the optimum for enzyme activity was pH 5. In contrast, invertase and bFFase were stable below 55 and 50 ?C, respectively, but showed highest activity at 65 and 60 ?C, respectively. Both enzymes were stable over the range pH 4–9 and had optimum activity at pH 4 for invertase and pH 5 for bFFase. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Production of ?-Fructofuranosidase from Aspergillus niger ATCC 20611) ผู้เขียน:ดร.สาโรจน์ ศิริศันสนียกุล, ศาสตราจารย์, Sanya Jitbanjongkit, Nararit Prasomsart, Pairojana Luangpituksa สื่อสิ่งพิมพ์:pdf AbstractAn increase in magnesium sulfate decreased the ratio of extracellular/intracellular ?- fructofuranosidase activity in shaking flask cultivation of Aspergillus niger ATCC 20611. At 0.4% MgSO4 concentration, the enzyme ratio was decreased to 54%, but the total enzyme activity was increased 1.12 times more than that with no salt addition. Three ammonium salts tested, (NH4)2SO4, (NH4)2HPO4, and NH4NO3 could not substitute yeast extract as a nitrogen source because of decreasing in the specific growth rate, enzyme yield and productivity. In this study, yeast extract with vitamin solution gave the highest values of specific growth rate, enzyme yield and productivity. Based on the fermentation kinetic study in aerobic batch culture under controlled pH and temperature, the specific growth rate, enzyme yield and productivity were improved to 0.0414 h-1, 65.16 ? 103 U/g sucrose and 1.89 ? 103 U/g cell h, respectively. These values were increased 1.85, 10.5 and 497.9 times, respectively, comparing with shaken flask culture. |
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หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Enzymatic Production of Fructo-Oligosaccharides from Sucrose) ผู้เขียน:ดร.สาโรจน์ ศิริศันสนียกุล, ศาสตราจารย์, Sittiwat Lertsiri, Preecha Tonsagunrathanachai, Pairojana Luangpituksa สื่อสิ่งพิมพ์:pdf AbstractTo increase an efficiency of fructo-oligosaccharide production from sucrose, a mixed-enzyme system of ?-fructofuranosidase (Aspergillus niger ATCC 20611) and glucose oxidase (Sigma Chemical Co., Ltd.) was carried out in a 2-l stirred tank reactor with the following controlled conditions; temperature 40?C, pH 5.5, aeration rate 1 vvm and agitation rate 550 rpm, for 32 hours of incubation time. With an initial sucrose concentration of 400 g/l, the optimal condition was obtained by using ?-fructofuranosidase and glucose oxidase 10 and 15 units per gram sucrose, respectively. As a result, the yields of kestose (YGF2/S) and nystose (YGF3/S) from sucrose and their volumetric productivities (QGF2/S and QGF3/S) were 0.44, 0.49 g/g, and 4.97, 5.44 g/l h, respectively. |
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Researcherดร. พิลาณี ไวถนอมสัตย์ที่ทำงาน:ฝ่ายนาโนเทคโนโลยีและเทคโนโลยีชีวภาพ สถาบันค้นคว้าและพัฒนาผลิตผลทางการเกษตรและอุตสาหกรรมเกษตร สาขาที่สนใจ:Molecular Biology, Enzyme Technology , การใช้ประโยชน์ลิกโนเซลลูโลสเพื่อพลังงานทดแทน Resume |
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