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การประชุมวิชาการScreening, production and characterization of a hydrophobin protein from white rot fungiผู้แต่ง: Nianrawan Meecharoen,  Dr.Chetsada Pothiratana, Assistant Professor , Dr.Surachai Thachepan, Assistant Professor , Dr.Lerluck Chitradon, Associate Professor, Dr.churapa teerapatsakul, Assistant Professor , การประชุมวิชาการ: |
 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Biodegradation of Lignin in Oil Palm Fronds by White Rot Fungi) ผู้เขียน:Penpaka Namoolnoy, Sivawan Phoolphundh, นางอาภรณ์ วงษ์วิจารณ์ สื่อสิ่งพิมพ์:pdf AbstractOil palm fronds are a by-product from the harvest of the fresh fruit from oil palms. Nowadays, oil palm fronds are used as a source of roughage for ruminants. However, the effective use of the fronds is limited by poor nutrient composition, mainly due to their high lignin and low protein levels. White rot fungi, which degrade cellulose, hemicellulose and lignin, are widely used to increase the digestibility of agro-residues. In this research, 63 fungal isolates of white rot fungi in Thailand were sampled and screened for their production of ligninolytic enzymes by the agar plate screening method. The results showed that 48 isolates could produce laccase, manganese peroxidase and/or lignin peroxidase. A total of 27 isolates that exhibited high ligninolytic enzyme activity were selected to study the lignin degradation in oil palm fronds by solid-state fermentation. It was found that only 7 isolates: OP04, OP06, OP13, OP16, OP47, OP53 and OP61 could reduce lignin in oil palm fronds from 30% down to 15–18% within 30 d at 30 ?C and OP06 showed the highest laccase and manganese peroxidase activities (mean ? SD) of 0.896 ? 0.02 and 0.799 ? 0.07 U/g of dry weight, respectively, where one unit (U) of enzyme activity was defined as the amount of enzyme catalyzing the oxidation of 1 ?mol of substrate per minute. |
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ที่มา:IWA 4th International Conference on Oxidation Technologies for Water and Wastewater Treatmentหัวเรื่อง:Comparison of White-rot Fungi and Ozone for Decolorization of wastewater from Pulp and Paper Industry |
ที่มา:IWA 4th International Conference on Oxidation Technologies for Water and Wastewater Treatmentหัวเรื่อง:Comparison of White-rot Fungi and Ozone for Decolorization of wastewater from Pulp and Paper Industry |
ที่มา:สำนักงานพัฒนาวิทยาศาสตร์และเทคโนโลยีแห่งชาติ (สวทช.)หัวเรื่อง:การกำจัดสีจากน้ำเสียของโรงงานผลิตเอทานอลจากกากน้ำตาลโดยใช้เทคนิคการตรึงเซลราไวท์รอลแบบลูกผสม หัวหน้าโครงการ:ดร.ณัฐกานต์ นิตยพัธน์, รองศาสตราจารย์ |
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หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Combination of Laccase, Xylanase and Cellulase in Lignocellulose
Degradation by White Rot Fungi, Lentinus polychrous Lev. and
L. squarrosulus Mont.) ผู้เขียน:Charida Pukahuta, นางพูนพิไล สุวรรณฤทธิ์, รองศาสตราจารย์, Emiko Shianagawa, Hisashi Hoshida, Yoshinori Nishizawa สื่อสิ่งพิมพ์:pdf AbstractThe 22 isolates of white rot fungi, Lentinus spp. were screened for their activity of cellulase,
xylanase and laccase. L. polychrous Lev. LP-PT-1 was cultured in 8 liquid media and 2 solid media. These
10 media were tested for lignocellulose degradation. Potato dextrose broth (PDB) and Fahraeus broth
with 2 % rice straw (FRS) were selected as appropriate media. Both media were conducted to cultivate
4 isolates of L. polychrous Lev. LP-PT-1, LP-SW-3, LP-WR-13 and LP-UD-1 and 1 isolate of L.
squarrosulus Mont.LS-YA. The xylanase and cellulase activities were determined at 40?C. Xylidine
induced higher laccase actvity in 4 isolates, with the exception of LP-PT-1. LP-PT-1 produced three
enzyme actvities in both PDB and FRS with and without xylidine addition. LP-SW-3 produced the highest
activity of xylanase and laccase, 13.1 mU/ml and 3.27 U/ml, respectively, in FRS with xylidine addition.
LP-SW-3, LP-WR-13 and LP-UD-1 showed the activity of three enzymes in FRS but lacked cellulase
and/or xylanase activities in PDB. The production of xylanase of LP-SW-3 in FRS was induced by rice
straw and of LP-UD-1 in PDB was induced by xylidine. LS-YA had the activity of all three enzymes in
PDB but lacked xylanase activity in FRS without xylidine addition. FRS and xylidine addition was used
to produce the culture broth for bleaching pulp. The increase in brightness of the treated pulp, 1.5-2.0 %
higher than the control pulp, was correlated with the detected activity of xylanase of L. polychrous
Lev.LP-PT-1, LP-UD-1 and L. squarrosulus Mont.LS-YA with low activity of laccase in each isolate.
The high and moderate activity of xylanase with high and moderate activity of laccase in L. polychrous
Lev. LP-SW-3 and LP-WR-13 did not increase the brightness of the treated pulp. It was concluded that
these thermotolerant white rot fungi have the different combination of enzyme systems on lignocellulose
degradation. The bleaching of pulp beneficially effected by the xylanase activity only when the activity
of laccase was low. |