 |
|
|
 |
|
|
ผลงานตีพิมพ์ในวารสารวิชาการHistology and Glycoconjugates Histochemistry in the Small and Large Intestinal Epithelium of the Malayan Pangolin, Manis javanicaผู้แต่ง: Dr.Apinun Suprasert, Professor , Mrs.Maleewan Liumsiricharoen, Associate Professor , Ms.Pakawadee Pongket, Dr.Teerasak Prapong, Assistant Professor , Mrs.Apantree Chantong, Mrs.Nathanan Prompa, วารสาร: |
 |
|
 ที่มา:วารสาร คณะวิทยาการจัดการ วิทยาเขตศรีราชาหัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Studies on Histochemistry of Glycoconjugates in mandibular Salivary glands of Buffaloes and other Ruminants) ผู้เขียน: สหเทพ จันทรวิมล, ดร.อภินันท์ สุประเสริฐ, ศาสตราจารย์ สื่อสิ่งพิมพ์:pdf Abstract |
|
 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Histochemical Detection of Glycoconjugates in the Anterior Lingual Salivary Glands of the Domestic Fowl) ผู้เขียน:Surapong Arthitvong, Natanun Makmee, ดร.อภินันท์ สุประเสริฐ, ศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractGlycoconjugates in secretory epithelium of the anterior lingual salivary glands of chicken have been studied by means of light microscopic histochemical methods. A series of staining procedures were employed including peroxidase - labeled lectin - diaminobenzidine methods and digestion techniques with neuraminidase or ? - amylase. The terminal portion of the anterior lingual gland consisted of mucous and seromucous cells. All the mucous cells in the glands possessed a mixture of glycoconjugates of different nature, but they could be divided into three types, on the basis of the principle type of glycoconjugates involved, namely primarily sulfated, carboxylated and neutral glycoconjugate-containing cells. The first mentioned type of cells was predominant. The presence of sialic acid, ? - D - mannose, ? - D - glucose and ? - D - galactose in these glycoconjugates was confirmed. The seromucous cells found in the anterior lingual gland contained a very small amount of comparable carbohydrates predominantly in the apical cytoplasm. Possible functional activities of the glands were discussed. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Lectin Histochemical Characterization of Glycoconjugates Present in Abomasal Epithelium of the Goat) ผู้เขียน:ดร.อภินันท์ สุประเสริฐ, ศาสตราจารย์, ดร.อุไร พงศ์ชัยฤกษ์, ผู้ช่วยศาสตราจารย์, นางสาวผกาวดี พงษ์เกษ, Takao Nishida สื่อสิ่งพิมพ์:pdf AbstractIn the abomasal epithelium of the goat, glycoconjugates have been studied by means of selective histochemical methods. The staining procedures employed were alcian blue (AB) pH 1.0 AB pH 2.5, Periodic acid Schiff (PAS) and AB pH 2.5-PAS. In addition twelve lectins, Triticum vulgaris WGA, Concana valin A, Glycine max SBA, Dolichos biflorus DBA, Arachis hypogea PNA, Solanum tuberosum STL, Datura stramonium DSA, Ulex europeus UEA-I, Bauhinia purpurea BPA, Ricinus communis RCAI, Lotus tetragonolobus LTA, and Limulus polyphemus LPA were applied to detect saccharides residues. According to the results obtained, the nature of glycoconjugates in mucous epithelium was found to change during cell migration from deep to superficial part. The surface mucous cells contained primarily neutral glycoconjugates with vicinal diol groupings and terminal fucose residues. By contrast, the mucous cells in deep pits and in pyloric glands contained acidic sulfated glycoconjugates with terminal sialic acid residues. The ?-D-mannose, ?-D-glucose, ?-D-galactose, N-acetylglucosamine and N-acetylgalactosamine were also found at the pyloric gland cells while they were smaller in amount at the surface mucous cells. The data suggested that changes of epithelial cells were closely correlated with cellular maturation. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Lectin Histochemistry of Glycoconjugates in Mandibular Gland of Chicken) ผู้เขียน:ดร.อภินันท์ สุประเสริฐ, ศาสตราจารย์, Surapong Arthitvong, ดร.เสรี กุญแจนาค, ผู้ช่วยศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractThe distribution of glycoconjugates in the chicken mandibular gland was studied by means of light microscopic histochemical methods. The staining procedures employed were horseradish peroxidase conjugated lectin, Alcian blue pH 2.5-periodic acid-Schiff (AB pH 2.5-PAS), and high iron diamine-alcian blue pH 2.5 (HID-AB pH 2.5). The lectins used in the present study were Concanavalin A (Con A), Ricinus communis agglutinin-I (RCA-I), wheat germ agglutinin (WGA), Dolichos biflorus agglutinin (DBA), Ulex europeues agglutinin - I (UEA-I), Limax flavus agglutinin (LFA), Lotus tetragonolobus agglutinin (LTA) and peanut agglutinin (PNA). According to the results obtained, acidic and neutral glycoconjugates with ?-D-mannose, ?- D-glucase, ?-D-galactose, N-acetyl-D-glucasamine, ?-L-fucose and sialic acid residues were visualized in the secretory cells of chicken mandibular gland. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Characterization of Glycoconjugates in the Sublingual Salivary Gand of Malayan Pangolin (Manis javanica )) ผู้เขียน:รุฮานี หมีนยะลา, นางมาลีวรรณ เหลี่ยมศิริเจริญ, รองศาสตราจารย์, นางสาวผกาวดี พงษ์เกษ, ดร.ธีระศักดิ์ พราพงษ์, ผู้ช่วยศาสตราจารย์, ดร.อภินันท์ สุประเสริฐ, ศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractThe sublingual salivary gland of Malayan pangolin(Manis javanica) was investigated by using conventional and lectin histochemical methods in combination with enzyme digestion procedures. The results showed both mucous and serous cells in secretory endpieces of sublingual salivary gland. Mucous cells showed a large number of vicinal diol groups with acid and neutral glycoconjugates. Lectin histochemistry gave evidence of the presence of mannose, ? N - acetylgalactosamine, N- acetylglucosamine and sialic acid linked to Galactosyl (?13) N – acetylgalactosamine residues. Serous cells had positive reaction with almost all the conventional employed, with the exception of acid glycoconjugates which were less diffused. Serous cells secreted predominantly neutral glycoconjugates with mannose, Galactosyl (?14 ) N- acetylglucosamine, N- acetylglucosamine and fucose residues. |
ที่มา:เชียงใหม่สัตวแพทยสารหัวเรื่อง:การตรวจหาเนื้อเยื่อเคมีไกลโคคอนจูเกตในต่อมนำลายกกหูของลิ่นชวา |
|
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Histochemical Detection of Glycoconjugates in Colonic Epithelium of the Goat) ผู้เขียน:นางสาวผกาวดี พงษ์เกษ, Suppalak Romrattanapun, นางมาลีวรรณ เหลี่ยมศิริเจริญ, รองศาสตราจารย์, ดร.ดลดา ศรีใส, อาจารย์, ดร.อภินันท์ สุประเสริฐ, ศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractThe distribution of glycoconjugates in the goat descending colon was studied by means of light microscopic histochemical methods. The staining procedures employed were horseradish peroxidase conjugated lectin, alcian blue (AB) pH 2.5, periodic acid-Schiff (PAS), ABpH 2.5-PAS, high iron diamine (HID), HID-ABpH 2.5. The lectins used in the present study were concanavalin A (ConA), Ricinus communis agglutinin I(RCA-I), Solanum tuberosum agglutinin (STL) and Limulus polyphemus agglutinin(LPA) . The result revealed that descending colonic epithelium of the goat contained acid sulfated glycoconjugates and neutral glycoconjugates with ?-D-mannose, ?-D-glucose, ?-D-galactose, Nacetylglucosamine and sialic acid residues. The quantity of neutral glycoconjugates with ?-D-mannose, ?-D-glucose, ?-D-galactose and N-acetylglucosamine increased from mucosal crypt up to mucosal surface of the epithelium, while sialic acid residues and sulfated glycoconjugates decreased from mucosal crypt to mucosal surface. |
|
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Histochemical Characterization of Glycoconjugates Present in Cloacal Epithelium of the Chicken) ผู้เขียน:ดร.อภินันท์ สุประเสริฐ, ศาสตราจารย์, นายบุญธรรม จงเจริญ, รองศาสตราจารย์, Suppaluk Romrattanapun สื่อสิ่งพิมพ์:pdf AbstractThe distribution of glycoconjugates in the chicken cloaca was studied by means of light microscopic histochemical methods. The staining procedures employed were horseradish peroxidase conjugated lectin, Alcian blue (AB) pH 1.0, AB pH 2.5, periodic acid –Schiff (PAS), AB pH 2.5-PAS in combination with enzyme neuraminidase digestion. The lectin used in the present study were peanut agglutinin (PNA), wheat germ agglutinin (WGA), Dolichos biflorus agglutinin (DBA), and Lotus tetragonolobus agglutinin (LTA) The mucosa of both coprodeum and urodeum is covered by simple columnar epithelium in which 2 type of cells are encountered-columnar and goblet cells. In contrast, one layer of only columna cells is found in the mucosa of proctodeum. Tubular glands of proctodeum are commonly provided at submucosal layer. All the goblet cells in coprodeum and urodeum and tubular glands of proctodeum were coloured blue and purple with AB pH 1.0 and AB pH 2.5-PAS, respectively. They were furthermore stained moderately with WGA. Neuraminidase digestion changed the blue coloration with AB pH 2.5 to pale blue. Removal of sialic acid with neuraminidase imparted weak to strong affinity of PNA. Histophysiological significances of glycoconjugates involved in the chicken cloaca were discussed with special reference to the functional activities of the carbohydrates. |