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ผลงานตีพิมพ์ในวารสารวิชาการMolecular cloning and overexpression of DGA1, an acyl-CoA-dependent diacylglycerol acyltransferase, in the oleaginous yeast Rhodosporidiobolus fluvialis DMKU-RK253ผู้แต่ง: Pirapan Polburee, Takao Ohashi, Yung-Yu Tsai, Thitinun Sumyai,  Dr.Noppon Lertwattanasakul, Assistant Professor , Dr.Savitree Limtong, Professor , Kazuhito Fujiyama, วารสาร:  
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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Selection and Optimization for Lipid Production of a Newly Isolated Oleaginous Yeast, Rhodosporidium toruloides DMKU3-TK16) ผู้เขียน:ภควัตร ไกรสินธุ์, ดร.วิเชียร ยงมานิตชัย, อาจารย์ , ดร.สาวิตรี ลิ่มทอง, ศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractRhodosporidium toruloides DMKU3-TK16, an oleaginous yeast, was isolated from soil by an enrichment technique in nitrogen-limited medium I. Lipid production by this strain was highest in shaking flask cultivation at 150 rpm and 28?C in nitrogen-limited medium II containing per liter 70 g glucose, 0.75 g yeast extract, 0.55 g (NH4)2SO4, 0.4 g KH2PO4, 2.0 g MgSO4•7H2O, with a pH of 5.5. Under these optimal conditions, when the C/N ratio of the medium was 140, R. toruloides DMKU3-TK16 produced a lipid quantity of 9.26 g/L, which was 71.30 % of the dry biomass (13.33 g/L) after 168 h of cultivation. The major fatty acids of the cellular lipid were oleic acid (41.54%), palmitic acid (22.49%), linoleic acid (15.12%) and steric acid (14.56%). |
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หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Selection and Optimization for Lipid Production of a Newly Isolated Oleaginous Yeast, Rhodosporidium toruloides DMKU3-TK16) ผู้เขียน:ภควัตร ไกรสินธุ์, ดร.วิเชียร ยงมานิตชัย, อาจารย์, ดร.สาวิตรี ลิ่มทอง, ศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractRhodosporidium toruloides DMKU3-TK16, an oleaginous yeast, was isolated from soil by an enrichment technique in nitrogen-limited medium I. Lipid production by this strain was highest in shaking flask cultivation at 150 rpm and 28?C in nitrogen-limited medium II containing per liter 70 g glucose, 0.75 g yeast extract, 0.55 g (NH4)2SO4, 0.4 g KH2PO4, 2.0 g MgSO4•7H2O, with a pH of 5.5. Under these optimal conditions, when the C/N ratio of the medium was 140, R. toruloides DMKU3-TK16 produced a lipid quantity of 9.26 g/L, which was 71.30 % of the dry biomass (13.33 g/L) after 168 h of cultivation. The major fatty acids of the cellular lipid were oleic acid (41.54%), palmitic acid (22.49%), linoleic acid (15.12%) and steric acid (14.56%). |
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