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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Production of Polyclonal Antibodies for Detection of Papaya ringspot virus (PRSV) in Ivy Gourd (Coccinia grandis (L.)) ผู้เขียน: Sukanya Noochoo, Wanpen sritongchai,  ดร.ศรีเมฆ ชาวโพงพาง, ผู้ช่วยศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractThe coat protein (CP) gene from the Papaya ringspot virus (PRSV) strain Chiang Mai 1 (PRSV-CP) was cloned and expressed in Escherichic coli and purified using nickel-nitrilotriacetic acid agarose resin. The recombinant coat protein was used as an antigen to produce polyclonal antibodies for detection of PRSV in ivy gourd. The antibodies were able to detect the recombinant protein and PRSV-infected plant samples. Specificity of the antibodies to PRSV was compared with indirect enzyme-linked immunosorbent assay (ELISA), dot-blot immunobinding assay (DIBA) and tissue blot immunoassay (TBIA). It was found that the antibodies were highly specific and could be used to detect the presence of virus in different parts of ivy gourd. The antibodies were used to detect PRSV infection in ivy gourd using indirect ELISA and DIBA on 211 samples collected from papaya orchards. It was shown that 107 samples (50.71%) were infected by PRSV. In this study, results from germination testing on 195 seedlings from the infected ivy gourd seeds implied that PRSV was not seed transmitted. Furthermore, the results from TIBA revealed that PRSV spread to all parts of the ivy gourd (leaf, flower, flower stalk, stem and root, as well as tendril) even though some of the infected parts did not show any viral symptoms. |
ที่มา:Agricultural Science Journal (วารสารวิทยาศาสตร์เกษตร)หัวเรื่อง:การตรวจวิเคราะห์และประเมินความต้านทานต่อโรค ในมะละกอดัดแปลงพันธุกรรมที่ได้รับการถ่ายยีน cost protein หรือ polymerase ของเชื้อไวรัสใบด่างจุดวงแหวนของมะละกอ |
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หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Proteomics of Papaya ringspot virus-Infected Papaya Leaves) ผู้เขียน:Wanwisa Siriwan, Sittiruk Roytrakul, Motoyuki Shimizu, Naoki Takaya, ดร.ศรีเมฆ ชาวโพงพาง, ผู้ช่วยศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractThe Papaya ringspot virus (PRSV) causes severe economic losses in both papaya and cucurbits throughout the tropical and subtropical regions. An understanding of the interaction between the papaya plant and PRSV can help to improve papaya production. The protein profi les of virus-infected and healthy papaya leaves were compared by two-dimensional polyacrylamide gel electrophoresis. Among the observed 490 protein spots, 227 were identifi ed using matrix-assisted laser desorption/ionizationtime of fl ight mass spectrometry and liquid chromatography-mass spectrometry. Forty-three proteins were found to be similar to those identifi ed in the Papaya EST and NCBI nr databases. They play roles in the areas of: photosynthesis (14%), photorespiration (5%), metabolism (20%), gene and biosynthesis (10%), defence related (7%), stress response (5%), signal transduction (10%), and unknown processes (29%). Spot intensity and transcription levels determined by real-time polymerase chain reaction showed ribulose-1, 5-bisphosphate carboxylase, Rieske protein ubiquinol cytochome C and chlorophyll A/B binding were down-regulated in infected plants. On the other hand, ubiquitin-like modifi ers, vascular processing enzyme and germin-like protein were up-regulated in infected plants at transcription and translation levels. The results showed the novel virus-responding mechanism of the papaya plant that might be essential for developing viral-tolerant papaya in the agricultural industries. |
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ที่มา:วารสารวิทยาศาสตร์เกษตรหัวเรื่อง:การตรวจสอบการผลิตโปรตีนชนิดใหม่ของมะละกอแขกนวลดัดแปลงพันธุกรรมที่ต้านทานไวรัสใบด่างวงแหวน |
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