ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Accumulation of Glycinebetaine and Betaine Aldehyde Dehydrogenase Activity in Eucalyptus camaldulensis Clone T5 Under in vitro Salt Stress)

ผู้เขียน:ศยามล สิทธิสาร, ดร.ปุณฑริกา หะริณสุต, รองศาสตราจารย์, Somchai Pornbunlualap, Suriyan Cha-um

สื่อสิ่งพิมพ์:pdf

Abstract

A widely distributed adaptation to counteract abiotic stress in plant is an accumulation of compatible organic solutes. There are many different types of salts and almost an equally diverse set of salt-tolerant defense mechanisms. Glycinebetaine (GB) is a major organic osmolyte that accumulates in a variety of plant species in response to salt stress. This research focused on the elucidation of the GB accumulation in the E. camaldulensis clone T5, under NaCl salinity. The results showed that there was a high correlation between GB accumulation and NaCl concentration, for a range of 0 to 500 mM NaCl. The biosynthesis of GB in the plant involved betaine aldehyde dehydrogenase (BADH) as a key enzyme, which also increased as the concentration of NaCl increased. The BADH protein contents in the extract were analyzed by SDS-PAGE gel. The result showed BADH protein expression with molecular mass 60 kDa. BADH activity was correlated with an accumulation of GB in response to salt stress. Total chlorophyll content in multiple shoots was unchanged in the first 13 days with 200 mM NaCl.

Article Info

Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 043, Issue 5, Dec 09 - Dec 09, Page 146 - 152 |  PDF |  Page