 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Factors Affecting Growth and ?-carotene Content of Chlorosarcinopsis sp. (PSU/CHL20) in Batch Culture) ผู้เขียน: Tassnapa Wongsnansilp, Pimpan Tansakul, Manop Arunyanart สื่อสิ่งพิมพ์:pdf AbstractThe effect of light intensity, potassium nitrate concentrations and temperature on the growth
and ?-carotene content of Chlorosarcinopsis sp. (PSU/CHL20) were determined. In the first experiment,
algal cells were grown on a shaker in Erlenmeyer flasks containing 150 ml of NSIII medium and at a
shaking speed of 150 rpm. Potassium nitrate concentrations were 0, 0.1 and 0.2 g l-1. Light intensities
used were 60, 120 and 180 ?molm-2s-1. The light / dark cycle was 16:8 h and the temperature was
controlled at 25?C. The optimum light intensity and potassium nitrate concentration for algal growth
and ?-carotene content was 120 ?molm-2s-1 and 0.1 g l-1 which produced a maximum cell number of
138?105 cell ml -1and a ?-carotene content of 0.133 mg l-1, (0.012 pg cell-1) respectively. In the second
experiment, the effect of temperature on the growth and ?-carotene content was investigated. In this
experiment the light intensity was 120 mmolm-2s-1 and the potassium nitrate concentration was 0.1g
l-1, at temperatures of 25?C, 30?C and 35?C. Although statistically insignificant (P>0.05), the algal
growth increased slightly with increasing temperature. The highest cell numbers of 148x105 cell ml-1
were obtained at 30?C on day 14 of cultivation. The maximum ?-carotene content of 0.543 mg l-1 (0.052
pg cell-1) was achieved at 30?C and this was significantly higher than that of the culture grown at 25?C
(P<0.05). |