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 ผลงานตีพิมพ์ในวารสารวิชาการIdentification of potential candidate genes involved in the sex determination cascade in an aquatic firefly, Sclerotia aquatilis (Coleoptera, Lampyridae)ผู้แต่ง: Nguantad, S,  Dr.Pramote Chumnanpuen, Assistant Professor , Dr.Anchana Thancharoen, Assistant Professor , Ms.Wanwipa Vongsangnak, Associate Professor , Dr.Ajaraporn Sriboonlert, Assistant Professor , วารสาร: 
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ผลงานตีพิมพ์ในวารสารวิชาการAn Investigation of ZZ/ZW and XX/XY Sex Determination Systems in North African Catfish (Clarias gariepinus, Burchell, 1822)ผู้แต่ง:Dung Ho My Nguyen, Thitipong Panthum, Jatupong Ponjarat, Nararat Laopichienpong, Dr.Ekaphan Kraichak, Assistant Professor , Worapong Singchat, Syed Farhan Ahmad, Dr.Narongrit Muangmai, Assistant Professor , Dr.Surin Peyachoknakul, Associate Professor , Dr.Uthairat Na-Nakorn, Professor , Dr.Kornsorn Srikulnath, Associate Professor , วารสาร: |
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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Analysis of Sex Determination in Some Cycads Using Methylation- Sensitive Amplification Polymorphism (MSAP)) ผู้เขียน:ฐิติ กาญจนเกตุ, ดร.วิภา หงษ์ตระกูล, รองศาสตราจารย์ , ดร.นิตย์ศรี แสงเดือน, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractThis is the first report on the analysis of sex determination in cycads using MSAP technique. Prior works using cytogenetic techniques, molecular markers such as random amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP) did not clearly identify sex in cycads. This modified AFLP technique using isochizomer enzyme (MspI and HpaII) called methylationsensitive amplification polymorphism (MSAP) was carried out to assess the pattern of cytosine methylation in both sexes of Cycas and Zamia. Using seven pairs of primers, 364 bands some of which showing sex-specific were produced and classified into three groups. The first group was non-polymorphic markers, whereas the second group was chosen from the results of differentation ability of MspI and HpaII to cut the methylated sequences, but sex-different markers were not obtained. Markers in the third group were methylation-sensitive and they also showed some polymorphic patterns between the two sexes. We suggested that sex in cycads may be associated with DNA methylation, but further study is required to reach a conclusive result. |
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