 ผลงานตีพิมพ์ในวารสารวิชาการProduction of l-phenylalanine from glycerol by a recombinant Escherichia coliผู้แต่ง: Khamduang, M, Packdibamrung, K, Chutmanop, J, Chisti, Y,  Dr.Penjit Srinophakun, Professor , วารสาร:  
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ผลงานตีพิมพ์ในวารสารวิชาการChilling injury in mango fruit peel: Cultivar differences are related to the activity of phenylalanine ammonia lyaseผู้แต่ง:Chidtragool, S, Dr.Saichol Ketsa, Emeritus Professor , Bowen, J, Ferguson, IB, van Doorn, WG, วารสาร: |
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ผลงานตีพิมพ์ในวารสารวิชาการInvestigation on thermoresponsive behavior of biodegradable poly(ฮณ-glutamic acid)-graft-L-phenylalanine ethyl esterผู้แต่ง:Dr.AMORNRAT LERTWORASIRIKUL, Assistant Professor , Choothong, N.-O., Yoshida, H., Matsusaki, M., Kida, T., Dr.Apirat Laobuthee, Associate Professor , Akashi, M., วารสาร: |
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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Optimization of Medium Composition for L-phenylalanine Production from Glycerol using Response Surface Methodology) ผู้เขียน:เมธี ขำดวง, จรัญ ฉัตรมานพ, Kanoktip Packdibamrung, ดร.เพ็ญจิตร ศรีนพคุณ, ศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractL-phenylalanine was produced by genetically modified bacterium, Escherichia coli BL21(DE3), using glycerol as an alternative carbon source. Response surface methodology (RSM) involving central composite design (CCD) was adopted to evaluate the amount of L-phenylalanine produced. In this work, the optimum concentrations were determined of the major nutrients in the fermentation medium, which included glycerol, (NH4)2SO4, MgCl2, K2HPO4, KH2PO4, yeast extract and thiamine-HCl. Analysis using biomass weight (gL-1) and amino acid production (gL-1), indicated that the optimum medium composition and concentration for the biomass production were: glycerol 10 gL-1, (NH4)2SO4 10 gL-1, MgCl2 0.98 gL-1, K2HPO4 2.94 gL-1, KH2PO4 2.94 gL-1, yeast extract 0.878 gL-1 and thiamine-HCL 0.0878 gL-1, with a maximum biomass weight of 5.0 gDCWL-1. In addition, the optimum medium composition for L-phenylalanine production was: glycerol 10 gL-1, (NH4)2SO4 100 gL-1, MgCl2 0.64 gL-1, K2HPO4 1.91 gL-1, KH2PO4 1.91 gL-1, yeast extract 0.823 gL-1 and thiamine-HCl 0.0823 gL-1. The highest L-phenylalanine weight at the optimum nutrient concentration was 6.2 gL-1. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Preparation, Structural Characterization, Toxicity Test, and DNA Release Study of Low Molecular Weight Chitosan-g-L-Phenylalanine/DNA Complex) ผู้เขียน:ดร.รังรอง ยกส้าน, รองศาสตราจารย์ , Mitsuru Akashi สื่อสิ่งพิมพ์:pdf AbstractThe preparation of low molecular weight chitosan-g-L-phenylalanine (LMWCts-g-Phe) was successfully carried out by the coupling reaction using carbodiimide as a coupling agent. The complex of the LMWCts-g-Phe and DNA (LMWCts-g-Phe/DNA), prepared by a complex coacervation method, exhibited various shapes, i.e., sphere, square, rectangle, and rod with an average size of ~ 50-150 nm as observed by SEM and a zeta potential in the range from -5.9 to -8.9 mV. The in vitro cytotoxicity test showed that the LMWCts-g-Phe and LMWCts-g-Phe/DNA were less toxic to fibroblast cells with the %viability of 76-111. The in vitro release of DNA from the complex was very fast in high pH medium (tris buffer, pH 8.0) and completed within 3 days, while the sustained release was achieved in neutral (PBS, pH 7.4) and low pH media (citric acid/sodium citrate buffer, pH 3.0). |
 ที่มา:Carbohydrate Polymersหัวเรื่อง:Low molecular weight chitosan-g-l-phenylalanine: Preparation, characterization, and complex formation with DNA |
ที่มา:Journal of Industrial Microbiology and Biotechnologyหัวเรื่อง:- |
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ที่มา:Carbohydrate Polymersหัวเรื่อง:Low molecular weight chitosan-g-l-phenylalanine: Preparation, characterization, and complex formation with DNA |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Optimized Production of L-phenylalanine by Fermentation Using Crude Glycerol) ผู้เขียน:ดร.อนุสิษฐ์ ธนะพิมพ์เมธา, รองศาสตราจารย์ , กล้า ธานีโต, เมธี ขำดวง, Kanoktip Packdibamrung, ดร.เพ็ญจิตร ศรีนพคุณ, ศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractCrude glycerol, a by-product of the commercial production of biodiesel, was used directly as the carbon source to produce the essential amino acid L-phenylalanine in an optimized fermentation process involving the novel recombinant bacterium Escherichia coli BL21(DE3). In developing the optimal process, a Plackett-Burman experimental design was used to identify the components of the medium that most affected the production of the cell mass and L-phenylalanine. For the specified carbon source and with ammonium sulfate as the nitrogen source, the media components that were the most influential were: KH2PO4, K2HPO4, NaCl, MgCl2, CaCl2, CoCl2, CuSO4, Na2MoO4, ZnSO4 and yeast extract. A three-level and five-factor central composite experimental design was then used to identify the optimal concentrations of the most influential components of the medium and the optimal duration of the fermentation. Under the optimal combination of conditions for biomass production, a biomass concentration of 1.23 g/L was achieved in 32.43 h. Similarly, under the optimal conditions for production of L-phenylalanine, a metabolite concentration of 1.03 g/L was achieved in 30.29 h. A high correlation between the models’ predicted responses and the actual responses achieved in independent validation experiments confirmed the validity of the models. |