Search Result of "molting"

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งานวิจัย

เม็ดเลือดและกิจกรรมของเอนไซม์ในวงจรการลอกคราบของปูทะเล (2010)

หัวหน้าโครงการ:Imgดร.จินตนา สและน้อย, รองศาสตราจารย์

แหล่งทุน:ทุนอุดหนุนวิจัยมก.

ผลลัพธ์:วารสาร (3)

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ผลงานตีพิมพ์ในวารสารวิชาการ

Chitobiase, Proteinase, Glycogen and some Trace Elements during Molting Cycle of Mud Crab (Scylla serrata Forsk?l 1775)

ผู้แต่ง:ImgDr.Jintana Salaenoi, Associate Professor,

วารสาร:

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ผลงานตีพิมพ์ในวารสารวิชาการ

Responses of laying hens to induce molting procedures using cassava meal of variable length with or without recovery period

ผู้แต่ง:ImgDr.Nirat Gongruttananun, Associate Professor, ImgKanokporn Saengkudrua,

วารสาร:

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การประชุมวิชาการ

Gutathione-S-transferase in Molting Cycle of Mud Crab (Scylla serrata)

ผู้แต่ง:Imgภัทราวดี ศรีมีเทียน, ImgDr.Jintana Salaenoi, Associate Professor,

การประชุมวิชาการ:

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ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Lipid Peroxidation and Antioxidant Activity in Molting Stages of Mud Crab (Scylla serrata))

ผู้เขียน:Imgดร.จินตนา สและน้อย, รองศาสตราจารย์, ImgPakawan Setthamongkol, Imgภัทราวดี ศรีมีเทียน, Imgดร.เกรียงไกร สถาพรวานิชย์, ผู้ช่วยศาสตราจารย์

สื่อสิ่งพิมพ์:pdf

Abstract

Lipid peroxidation (LPO) is the process whereby oxygen free radicals steal electrons from polyunsaturated fatty acids of cell membranes, initiating a self-propagating chain reaction and the end-products of the reactions are dangerous to the viability of cells. It is used as an indicator of oxidative stress or the amount of free radicals in cells and tissues. The observation of LPO activities in various organs of mud crab (Scylla serrata) over the molting cycle showed evidence that supported the functions of free radicals. LPO in the intermolt stage was significantly (P < 0.05) lower than at the premolt stage. The LPO activities gradually increased when the crab entered the premolt stage which was detected in the haemolymph, gill, hepatopancreas, integument and muscle of crabs. The hepatopancreas seemed to be the organ where the free radical processes occurred. LPO in the haemolymph, integument and gills had the highest levels 2 d before molting while the activities in the hepatopancreas showed distinguishing functions 2 wk before molting. The determination of antioxidant activity using free radical scavengers showed the same pattern in the gills, meat, hepatopancreas and claws. The activities gradually decreased from the intermolt stage until they reached a minimum in the early postmolt, and then increased again at late postmolt. It clearly indicated that LPO activities in the molting cycle corresponded with the physiology during the stage changing and adaptation of crab.

Article Info
Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 049, Issue 3, May 15 - Jun 15, Page 422 - 432 |  PDF |  Page 

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ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Variation of Calcium, N-acetylglucosamine, Glucosamine and Glucose Content during Molting Cycle of Mud Crab (Scylla serrata Forsk?l 1775))

ผู้เขียน:Imgดร.จินตนา สและน้อย, รองศาสตราจารย์, ImgJitlada Bootpugdeethum, Imgดร.มิ่งขวัญ มิ่งเมือง, รองศาสตราจารย์, Imgนางอมรา ทองปาน, รองศาสตราจารย์

สื่อสิ่งพิมพ์:pdf

Abstract

Calcium, N-acetylglucosamine, glucosamine and glucose content in the tissues of mud crab (Scylla serrata Forsk?l 1775) during molting cycle were determined. The results showed that calcium in gill decreased gradually from intermolt (C) to the lowest level of 6,527 ppm at 2-day premolt (D3) and increased again from 6-h postmolt (A1) to the highest level of 24,058.50 ppm at 7-day postmolt (B2.3) which was similar in pattern to calcium in the integument but of which at lower range (1,360.10-8,044.30 ppm). The highest content in haemolymph was found at 2-day premolt (D3). Variation of Nacetylglucosamine, a major component of chitin, showed similar pattern to calcium. Their content in gill and integument decreased from intermolt (C) to premolt (D1-D3) and increased at postmolt stages (A1-B2.3) while the pattern was opposite to that found in haemolymph which increased at premolt (D1- D3) and decreased at postmolt stages (A1-B2.3). For glucosamine, however, the content was quite low in both gill and integument but was moderately high at late postmolt in haemolymph. Glucose, on the other hand, seemed to be quite stable throughout the molting cycle (averaged 0.8973 ? 0.1489 % mg) in integument, but the profiles in hepatopancreas and gill were similar having the highest level at 5-day postmolt (B2.2). Glucose in muscle was at its peak of 0.7265 ? 0.1016 % mg at 2-day premolt (D3) followed by the ten times high level in haemolymph of 12.2020 ? 0.4807 % mg at late postmolt (B2.1). Variations of calcium, N-acetylglucosamine, glucosamine and glucose content corresponded to the changing of chemical composition of the tissues during chitin degradation of the old shed, chitin biosynthesis, mineralization of the new structure and the transportation of these substances between tissues.

Article Info
Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 040, Issue 3, Jul 06 - Sep 06, Page 668 - 679 |  PDF |  Page 

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ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Chitobiase, Proteinase, Glycogen and some Trace Elements during Molting Cycle of Mud Crab (Scylla serrata Forsk?l 1775))

ผู้เขียน:Imgดร.จินตนา สและน้อย, รองศาสตราจารย์, ImgJitlada Bootpugdeethum, Imgดร.มิ่งขวัญ มิ่งเมือง, รองศาสตราจารย์, Imgนางอมรา ทองปาน, รองศาสตราจารย์

สื่อสิ่งพิมพ์:pdf

Abstract

Activity profiles of chitobiase, proteinase and the content of glycogen and trace elements in gill, integument and haemolymph of mud crab (Scylla serrata) during molting cycle were determined. Chitobiase activity was at the highest level of 0.5467?0.0136 ?mol min-1mg protein-1 at 1-week premolt (D2) in gill and at 0.1413?0.0026 ?mol min-1 mg protein-1 in integument but reached the maximum level of 0.0851?0.0123 ?mol min-1mg protein-1 at 24-h postmolt (A2.2) in haemolymph. The high activities of proteinase, on the other hand, were found to be 0.6511 ? 0.0934 units mg protein-1 at 7-day postmolt (B2.3), 0.1553 ? 0.0419 units mg protein-1 at 1-week premolt (D2), and 0.1827?0.0110 units mg protein-1 at 12-h postmolt (A2.1) in gill, integument and haemolymph, respectively. These two enzymes are needed for carapace degradation as well as for chitin synthesis in preparation for carapace formation. For glycogen content, high accumulation of glycogen in the integument was observed throughout the molting cycle except at intermolt (C). In gill, however, the level of glycogen was high only before molting began (D2) while glycogen content in hepatopancreas and muscle, energy reserve tissues, was rather fluctuated but reached the maximum levels at 24-h postmolt (A2.2). As for trace elements, copper, a major component of hemocyanin, was found at high level at intermolt stage (C) in all tissues while magnesium, involving both nerve conduction and muscle contraction, showed the highest content at 7-day postmolt (B2.3) and 1-week premolt (D2) in integument and haemolymph, respectively. On the other hand, zinc showed high content at premolt stage in haemolymph and early postmolt stage in gill supporting its function in calcification. The results confirmed the roles of chitobiase, proteinase, glycogen, and trace elements which corresponded well with molting stages and physiological changes of mud crab.

Article Info
Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 040, Issue 2, Apr 06 - Jun 06, Page 517 - 528 |  PDF |  Page 

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