Search Result of "lipid accumulation"

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การประชุมวิชาการ

Algal lipid accumulation under photoautotrophic cultivation with different nitrogen levels by Chlorella sp. TISTR 8990

ผู้แต่ง:ImgNatthawut Yodsuwan, ImgDr.Sarote Sirisansaneeyakul, Professor,

การประชุมวิชาการ:

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ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Enhancement of Growth and Lipid Accumulation by Chlorella sorokiniana 1019 under Mixotrophic Culture Conditions)

ผู้เขียน:Imgศิริวัฒน์ คูเจริญไพบูลย์, Imgดร.สาวิตรี ลิ่มทอง, ศาสตราจารย์, Imgดร.วิเชียร ยงมานิตชัย, อาจารย์

สื่อสิ่งพิมพ์:pdf

Abstract

The enhancement of the growth and lipid accumulation by a microalga, Chlorella sorokiniana 1019, was investigated under mixotrophic conditions. The results showed that the growth and lipid production in this alga were notably increased under mixotrophic conditions using a limited-nitrogen medium compared to phototrophic and heterotrophic conditions. The biomass yield and lipid production were approximately 3.9 and 2.6 times more than under phototrophic growth. Among the three carbon sources of glucose, potassium acetate and glycerol, 1% glucose promoted the highest growth rate at 7.58 g of cell dry weight.L-1 after 7 d of cultivation, while 3% acetate yielded the highest cellular lipid reaching 56% of the dried biomass. The fatty acid composition of the alga was slightly changed from the predominant linoleic acid in the glucose medium to palmitic acid in the acetate medium.

Article Info
Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 048, Issue 3, May 14 - Jun 14, Page 433 - 441 |  PDF |  Page 

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ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Outdoor Photoautotrophic Cultivation of Chlorella sp. TISTR 8990 in Nitrogen- and Phosphorus-Minimal Media for Lipid Accumulation)

ผู้เขียน:Imgณัฐวุฒิ ยอดสุวรรณ, ImgNahatai Chamchuang, Imgโยธกา ปัชชา, Imgดร.สาโรจน์ ศิริศันสนียกุล, ศาสตราจารย์

สื่อสิ่งพิมพ์:pdf

Abstract

The nitrogen/phosphorus pool available during phases of microalgal growth plays an important role in obtaining higher biomass and lipid accumulation. The current study was based upon the optimization of the microalga, Chlorella sp. TISTR 8990 using a photoautotrophic culture in an open 6 L drinking water bottle with a total working volume of 4 L and continuous sparging with 1% (v/v) CO2 mixed air at a flow rate of 0.67 volume per liquid volume per minute. The effects were investigated of nitrogen (0, 258.5, 317.0 and 475.5 mg.L-1 KNO3 at fixed 1 g.L-1 KH2PO4) and phosphorus (0, 19.4, 38.8 and 58.2 mg.L-1 KH2PO4 at fixed 2 g.L-1 KNO3) concentrations on the biomass and lipid production. The results showed that 258.5 mg.L-1 KNO3 and 38.8 mg.L-1 KH2PO4 maximized the cell concentrations after 144 hr of cultivation at 473.7 and 455.0 mg.L-1, respectively. On the other hand, 31% and 22% lipid contents were obtained in the basal medium with 475.5 mg.L-1 KNO3 and 38.8.mg.L-1 KH2PO4, respectively. The biomass concentration and lipid content were highest in the nitrogen-minimal medium, but at different initial concentrations of KNO3 (258.5 and 475.5 mg.L-1 KNO3, respectively). In addition, palmitic acid (C16:0), linoleic acid (C18:2) and linolenic acid (C18:3) were mostly found in the microalgal oil obtained from all treatments. It was concluded that KNO3 and KH2PO4 in the basal media assisted in producing the optimal microalgal growth and lipid accumulation in cells. However, the nitrogen-minimal medium showed potential for lipid production on the basis of the lipid content. In addition, the biodiesel quality specified using the saponification number, iodine value and cetane number were calculated from the fatty acid methyl ester composition. It was also found that fatty acids derived from the nitrogen-minimal medium are a higher potential source for biodiesel production than those from the phosphorus-minimal medium.

Article Info
Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 049, Issue 1, Jan 15 - Feb 15, Page 80 - 91 |  PDF |  Page 

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ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Optimization of Lipid Accumulation by Starchless Mutant Chlorella sorokiniana for Biodiesel Production)

ผู้เขียน:ImgWatchara Jantasee, Imgดร.วิเชียร ยงมานิตชัย, อาจารย์, Imgดร.เดือนรัตน์ ชลอุดมกุล, ผู้ช่วยศาสตราจารย์

สื่อสิ่งพิมพ์:pdf

Abstract

Improvement of oil accumulation in Chlorella sorokiniana was conducted by ultraviolet mutagenesis. In total, 63 starchless mutants from over 40,000 colonies were screened using an iodine fumigation technique. Eight starchless mutants had relatively high levels of oil accumulation compared to wild type strains. The lipid content of starchless C. sorokiniana DMKU5202-31 increased from 15% to 21.16%. Therefore, this strain was selected for further optimization using a statistical experimental design. Seven factors were screened using a Plackett-Burman design and further optimized using a central composite design (CCD). The Plackett-Burman design presented three significant factors—KNO3, pH and light intensity—that affected biomass and lipid accumulation significantly. CCD was used to optimize the significant factors and indicated that the optimal values for KNO3, pH and light intensity were 0.9 g.L-1, 6.2 and 4,000 lux, respectively. The response surface plots revealed that the maxima for biomass, lipid production and lipid content were 2.58 g.L-1, 1.40 g.L-1 and 54.49%, respectively. Thus, it could be concluded that ultraviolet mutation and the statistical experimental design can be used to improve oil accumulation in C. sorokiniana. After mutation and optimization, strain DMKU5202 had a lipid content increase from 15% to 54.49%. Therefore, these techniques were very efficient for the development of an upstream process for biodiesel production.

Article Info
Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 049, Issue 1, Jan 15 - Feb 15, Page 54 - 66 |  PDF |  Page 

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ที่มา:การประชุมทางวิชาการ ครั้งที่ 50 มหาวิทยาลัยเกษตรศาสตร์

หัวเรื่อง:อิทธิพลของการให้ก๊าซคาร์บอนไดออกไซด์ต่อการเจริญของสาหร่ายขนาดเล็ก Chlorella sp. TISTR 8990 ภายใต้ระบบเปิดถาดเขย่า

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