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ผลงานตีพิมพ์ในวารสารวิชาการMinimal Susceptibility to Highly Pathogenic Avian Infl uenzaH5N1 Viral Infection of Pigeons (Columba livia) and PotentialTransmission of the Virus to Comingled Domestic Chickensผู้แต่ง: Dr.Thaweesak Songserm, Professor ,  Rassameepen Phonaknguen, Kridsada Chaichoun, Wittawat Wiriyarat, Ladawan Sariya, Natanan Prayoowong, Nattapat Chaisilp, Mrs.Pattra Moonjit , Pilaipan Puthavathana, Prasert Auewarakul, Parntep Ratanakorn, วารสาร: 
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 ที่มา:การประชุมวิชาการวิทยาศาสตร์และเทคโนโลยีเพื่อเยาวชน ครั้งที่ 5หัวเรื่อง:ความสัมพันธ์ของปริมาณน้ำสัมพัทธ์ และการรั่วของสารอิเล็กโทรไลต์จากภายในเซลล์กับการทนแล้งของหญ้ารูซี่ (Urochloa ruziziensis (R.Gem & C.M. Evrard) Crins) |
 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Radiosensitivity of Vetiver to Acute and Chronic Gamma Irradiation) ผู้เขียน:ดร.นวลฉวี รุ่งธนเกียรติ, ศาสตราจารย์ , Peranut Jompuk, Titirat Rattanawongwiboon, Ratchanewan Puingam สื่อสิ่งพิมพ์:pdf AbstractNuclear technology has been widely applied in agriculture for crop improvement. In this study, the radiosensitivities of native Thai vetiver, Chrysopogon zizaninoides, the Kamphaeng Phet 2 and the Surat Thani ecotypes, and Chrysopogon nemoralis, the Ratchaburi ecotype, were investigated with acute and chronic gamma irradiation. Vetiver tillers of the Kamphaeng Phet 2 and the Surat Thani ecotypes were exposed to acute irradiation with gamma radiation from Cs-137 at doses of 0, 10, 20, 30, 40, 50, 60, 80 and 100 Gy. For chronic irradiation with a Co-60 source, the Kamphaeng Phet 2 tillers were exposed to 0, 65, 104, 116, 157, 182 and 205 Gy whereas the Ratchaburi tillers were exposed to 0, 63, 87, 127, 150, 173 and 213 Gy. The survival rate and growth performance measured by plant height, shoot and root dry weight of vetiver at 90 d after irradiation were recorded for median lethal dose (LD50/90) and 50% growth reduction dose (GR50/90) determination. Gamma radiosensitivity differences were observed between the irradiation methods and between the vetiver ecotypes. Acute irradiation caused higher radiosensitivity of vetiver than chronic irradiation. The LD50/90 values of the Kamphaeng Phet 2 ecotype to acute and chronic irradiation were 82 and 100 Gy, respectively. In general, the survival rate and growth of vetiver decreased with an increase in the gamma radiation doses. However, chronic irradiation of the Kamphaeng Phet 2 ecotype at 65 Gy gave higher shoot and root dry weights than the control treatment (0 Gy). The GR50/90 values of the Kamphaeng Phet 2 and the Surat Thani ecotypes to acute irradiation were 48 and 75 Gy for plant height and were 30 and 43 Gy for total dry weight, respectively. For chronic irradiation, the GR50/90 values of the Kamphaeng Phet 2 and the Ratchaburi ecotypes were 118 and 109 Gy for plant height and 121 and 67 Gy for total dry weight, respectively. This information will be useful for radiation-induced mutations in vetiver research. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Minimal Susceptibility to Highly Pathogenic Avian Infl uenza H5N1 Viral Infection of Pigeons (Columba livia) and Potential Transmission of the Virus to Comingled Domestic Ch ผู้เขียน:Rassameepen Phonaknguen, Kridsada Chaichoun, Wittawat Wiriyarat, Ladawan Sariya, Natanan Prayoowong, Nattapat Chaisilp, นางภัทรา มูลจิตร, Pilaipan Puthavathana, Prasert Auewarakul, นายปานเทพ รัตนากร, ผู้ช่วยศาสตราจารย์ , ดร.ทวีศักดิ์ ส่งเสริม, ศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractIn order to elucidate the epidemic dissemination role of pigeons in the outbreak of the highly pathogenic avian infl uenza (HPAI) H5N1 (infl uenza A) virus, this study was conducted by experimental infection of the HPAI H5N1 virus in pigeons. Twenty-eight pigeons, aged 1 yr that were serologically negative for the H5N1-specifi c HI antibodies, were inoculated intranasally with various doses of the HPAI H5N1 virus at infectious doses of 10 to 1 ? 106 median tissue culture TCID50, this is the amount of dose that will produce pathological change in 50% of the cell cultures inoculated. To evaluate the virus transmission of infected pigeons to the environment, pigeons inoculated with 1 ? 105 TCID50 of HPAI H5N1 were determined for their transmission ability to sentinel avian infl uenza virus-free chickens. Viral isolation and real-time hydrolysis probe (TaqMan) reverse transcriptase polymerase chain reaction were performed to detect viruses from choanal cleft and cloacal swabs. Antibody responses were detected by hemagglutination inhibition and serum neutralization assay. In this study, the median infective dose (ID50) and the median lethal dose (LD50) of the HPAI H5N1 virus of inoculated pigeons were 1 ? 105 and 1 ? 106 TCID50, respectively. The viruses were also consistently isolated from either cloanal or cloacal swabs of the infected groups. The sentinel chickens housed in the same cage were infected with the HPAI H5N1 virus shedding from the experimental pigeons. The sentinel chickens exhibited clinical signs with high morbidity and mortality. The results showed that the pigeons were less susceptible to HPAI H5N1 virus infection than chickens. The pigeons might be play role as distributors of avian infl uenza virus transmission and shedding from the infected pigeons to contacted chickens. Therefore, the good biosafety and biosecurity management in farms should be emphasized for control and prevention of the HPAI H5N1 virus. |
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Researcherนาง ภัทรา มูลจิตรที่ทำงาน:ภาควิชาเวชศาสตร์และทรัพยากรการผลิตสัตว์ คณะสัตวแพทยศาสตร์ สาขาที่สนใจ:เทคนิคเนื้อเยื่อสัตว์ อิมมูโนฮิสโตเคมี, animal cells resource and applied technique, Karyotyping Resume |
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