 |
|
|
|
 |
|
|
ผลงานตีพิมพ์ในวารสารวิชาการEffect of pre-freeze semen quality, extender and cryoprotectant on the post-thaw quality of Asian elephant (Elephas maximus indicus) semenผู้แต่ง: P. Imrat,  Ms.Piyawan Suthunmapinanta , K. Saikhun, S. Mahasawangkul, E. Sostaric, P. Sombutputorn, S. Jansittiwate, Dr.Nikorn Thongthip, Associate Professor , Dr.Anuchai Pinyopummin, Associate Professor , B. Colenbrander, W.V. Holt, T.A.E. Stout, วารสาร:   
|
|
|
|
 |
|
 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Effects of Extender and Storage Time on Motility and Ultrastructure of Cooled-Preserved Boar Spermatozoa) ผู้เขียน:Duangjai Boonkusol, Kulnasan Saikhun, Poonsuk Ratanaphumma สื่อสิ่งพิมพ์:pdf AbstractArtificial insemination (AI) using fresh diluted semen is used worldwide in the porcine industry. Viability of spermatozoa in diluted semen depends on several factors, such as the interaction with the type of extender and storage duration. The aim of this study was to evaluate the effects of extenders and storage time on the motility and ultrastructure of cooled-preserved boar spermatozoa. Semen samples were collected, diluted in BTS, Merck III or Androhep and stored at 15?C for 0, 1, 3, 5 and 7 d. The samples were warmed (37?C) and spermatozoa were evaluated for motility using light microscopy and ultrastructure using scanning (SEM) and transmission (TEM) electron microscopy. The results showed that motility did not differ significantly (P>0.05) among extenders at days 0 to 3, while Androhep showed a significantly (P<0.05) higher percentage of motility than BTS and Merck III from days 5 to 7. At day 7 of semen storage, plasma and acrosomal membrane damage were observed, as revealed by SEM and TEM. These findings suggest that the type of semen extender and cooled-preserved time affected motility and caused structural damage to boar spermatozoa, especially in the plasma and acrosomal membrane. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Effects of Extender, Cryoprotectant and Freezing Protocols on Post-thaw Sperm Motility, Morphology and Viability of Three-yellow Cocks (Gallus domesticus) Spermatozoa) ผู้เขียน:วีระศักดิ์ ฟุ้งเฟื่อง, ดร.วรวิทย์ สิริพลวัฒน์, รองศาสตราจารย์ , Suchart Sa-nguanphan, ดร.อนุชัย ภิญโญภูมิมินทร์, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractSemen samples collected from 10 three-yellow cocks (Gallus domesticus) were pooled and treated to determine the effect of extender and cryoprotectant (experiment 1) and the effect of freezing protocol and cryoprotectant (experiment 2) on frozen/thawed semen quality. In the first experiment, semen samples were diluted 1:1 (v:v) with two different semen extenders, Beltsville poultry semen extender (BPSE) or modified Tyrode’s medium (TALP) and cryopreserved with either 6% dimethyl acetamide (DMA) or 8% dimethyl sulfoxide (DMSO). The best result (p<0.05) was obtained for TALP extender + 8% DMSO, with values of 57.32% for viability, 56.57% for normal morphology and 48.50% for motility. In experiment 2, two different freezing protocols (one-step and two-step) were evaluated. The results showed that there was no difference in post-thaw motility and viability of the spermatozoa (sperm) when either the one-step or the two-step protocol was applied with 8% DMSO. In contrast, the sperm motility and viability with the one-step protocol were better (p<0.05) than those with the two- step protocol when 6% DMA was used. This study indicated that from in vitro analysis, TALP with 8% DMSO was the preferred freezing solution for three-yellow cock sperm and this could be carried out using a one-step freezing protocol. |
 |
|
|
ที่มา:Thai J Vet Medหัวเรื่อง:ผลของสารละลายน้ำเชื้อต่อการเคลื่อนที่และรูปแบบการเคลื่อนที่ของน้ำเชื้อละมั่ง(Cervus eldii) แช่แข็งหลังการอุ่นละลาย |
|