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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Assessment of Genetic Diversity and Population Structure in Jute (Corchorus spp.) Using Simple Sequence Repeat (SSR) and Amplified Fragment Length Polymorphism (AFLP) Marker ผู้เขียน: Ranjit Kumar Ghosh, อรุณี วงษ์แก้ว,  ดร.ธานี ศรีวงศ์ชัย, รองศาสตราจารย์ , ดร.สุตเขตต์ นาคะเสถียร, รองศาสตราจารย์ , ดร.เฉลิมพล ภูมิไชย์, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractThe genetic diversity and population structure of 63 jute genotypes from C. capsularis L. and C. olitorius L. were investigated using simple sequence repeat (SSR) and amplified fragment length polymorphism (AFLP) markers. The average polymorphism information content (PIC) value for the SSR and AFLP marker systems was 0.41 and 0.47, respectively. Primer MJM136 for SSR and primer combination E-AGG/M-CTA for AFLP showed the highest PIC values (0.51 and 0.50, respectively), indicating they were the most informative primers for the assessment of genetic diversity in jute and that SSR and AFLP markers are useful for distinguishing jute genotypes. The PIC value for C. olitorius was relatively higher (0.45) than for C. capsularis (0.43). Cluster analysis based on the unweighted pair group method of arithmetic means clearly classified the genotypes of the two jute species into two main clusters. The results from the analysis of molecular variance revealed 81% molecular variation between species but it was low (19%) within species. The most diverse genotypes were identified as IND4546, TAN4231 and BRA4794 in C. olitorius and CVL-1, BAN2596C and CHI4995C in C. capsularis and these could be used as the most diverse genetic material in future breeding programs for jute improvement. |
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หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Identifying Quantitative Trait Loci for Fiber Content and Fiber Components in Sugarcane Using Amplified Fragment Length Polymorphism Markers) ผู้เขียน:วารีย์ เวรวรณ์, ดร.เรวัต เลิศฤทัยโยธิน, รองศาสตราจารย์ , ดร.ชัยณรงค์ รัตนกรีฑากุล, รองศาสตราจารย์ , ดร.ธานี ศรีวงศ์ชัย, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractThe cane yield, fiber content and fiber components (cellulose, hemicellulose and lignin) were determined from the segregation of 171 hybrid sugarcane clones derived from crossing K 84-200 and Kps 94-13 at 12 mth after planting. The results revealed that the average cane yield and fiber content in the hybrid sugarcane clones were 11.54 t.rai-1 (1 rai = 0.16 hectare) and 12.66%, respectively. The bagasse or fiber consisted of cellulose, hemicellulose and lignin with values of about 37.88–47.75, 30.15–40.73 and 4.81–10.23% dry weight, respectively. For amplified fragment length polymorphism identification, a total of 107 simplex markers were used to analyze the inheritance of quantitative trait loci (QTLs) for fiber, cellulose, hemicellulose and lignin contents. Eleven putative QTLs from the simplex markers were identified for these four traits. Each QTL explained from 2.5 to 4.3% of the variation in the fiber content. In parts of the fiber components, each QTL explained from 3.3 to 5.6% of the variation in the cellulose content and 2.3 to 3.0 % in the hemicellulose content, while the QTL explained 2.4 to 5.0 % of variation in the lignin content. Moreover, there were two putative QTLs related to more than one trait. |
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ที่มา:การประชุมทางวิชาการของมหาวิทยาลัยเกษตรศาสตร์ ครั้งที่ 46หัวเรื่อง:การตรวจ genotyping ของ Campylobacter jejuni และ Campylobacter coli ในฟาร์มไก่เนื้อและโรงฆ่าไก่ ด้วยวิธี amplified fragment length polymorphism |
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หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Amplified Fragment Length Polymorphism Analysis of Campylobacter jejuni and Campylobacter coli from Broiler Farms and Different Processing Stages in Poultry Slaughterhouses ผู้เขียน:เฉลิมเกียรติ แสงทองพินิจ, Sasitorn Kanarat, ดร.ธีระพล ศิรินฤมิตร, รองศาสตราจารย์ , ดร.ปฐมาพร เอมะวิศิษฎ์, รองศาสตราจารย์ , ดร.ธวัชชัย ศักดิ์ภู่อร่าม, ผู้ช่วยศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractThe aim of this study was to use the high-resolution genotyping method of amplified fragment length polymorphism (AFLP) to investigate the genetic diversity of Campylobacter jejuni and C. coli isolates obtained from broiler farms and different stages of slaughterhouse processing. Of 490 samples, 328 isolates of Campylobacter spp. were found (328/490, 66.9%) and the isolates were identified as C. jejuni (24.1%, 118/490) and C. coli (42.8%, 210/490). The genetic fingerprint of the 314 Campylobacter isolates, as determinated by AFLP, revealed 48 AFLP strains of C. jejuni and 95 AFLP strains of C. coli. In most flocks, a broad diversity of C. jejuni and C. coli strains was found and the distribution of AFLP types changed during slaughter line processing. Some genotypes were found to be the contamination source of both species in chicken intestines and by direct contamination of chicken meat during the slaughtering and cutting processes in the slaughterhouse. AFLP fingerprinting was an effective method to discriminate between C. jejuni and C. coli strains, in which the interlinkage homology of the AFLP pattern was only 35-42%. In addition, AFLP fingerprinting could distinguish between strains that were genetically unrelated or related. Therefore, AFLP analysis was considered a suitable epidemiological tool for investigation of Campylobacter. |
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