Search Result of "Yuttana Singchada"

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ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Sequence-Tagged Site of Defense-Related Genes for Resistant/ Susceptible Eucalypt Selection to Cryptosporiopsis eucalypti)

ผู้เขียน:ImgYuttana Singchada, Imgนางสาวเสาวนีย์ สุพุทธิธาดา, รองศาสตราจารย์, Imgนางพูนพิไล สุวรรณฤทธิ์, รองศาสตราจารย์, Imgดร.สมศักดิ์ อภิสิทธิวาณิช, รองศาสตราจารย์



Leaf spot and shoot blight caused by the fungal pathogen, Cryptosporiopsis eucalypti, is the most severe disease for eucalypt plantation in Thailand. The objective of this study was to create a set of DNA markers for resistant clone detection. Twelve clones of eucalypt were selected and screened for disease resistance using intact seedling inoculation and detached leaf techniques in the greenhouse and laboratory. Six clones, SF01, SF06, SF18, SF36, SF94 and SF98, were classified as resistance while the other six, SF03, SF07, SF14, SF16, SF70 and SF86, were susceptible clones. The sequence-tagged-site (STS) marker of pathogenesis-related (PR) genes, plant disease resistance (R) genes, and defense-related genes were analyzed in both DNAs of resistant and susceptible groups using polymerase chain reaction (PCR). Amplified DNA fragments were separated by agarose gel and polyacrylamide gel electrophoresis. The results showed that six markers (Ce1, Ce2, Ce3, Ce4, Ce5 and Ce6) from thirty five primer pairs could be used for susceptible and resistant detection of eucalypt.

Article Info
Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 040, Issue 3, Jul 06 - Sep 06, Page 748 - 754 |  PDF |  Page