ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : In Vitro Chromosome Doubling in Korarima [Aframomum corrorima (Braun) P.C.M. Jansen] Using Colchicine and Oryzalin)

ผู้เขียน:ดร.สุรวิช วรรณไกรโรจน์, รองศาสตราจารย์, Wondyifraw Tefera

สื่อสิ่งพิมพ์:pdf

Abstract

Ploidy manipulation holds high promise for the improvement of korarima (Aframomum corrorima) plants with altered morphology, as well as of potentially altered essential oil yields and oil composition. The present work aimed to establish a protocol for in vitro polyploid induction of korarima. Nodal explants 2 mm long were cultured in liquid media with 0, 125, 250, 375 or 500 ?M colchicine or 10, 20, 40, 60 or 80 ?M oryzalin for 7 d. The lowest concentration was the most effective for polyploid induction with both colchicine and oryzalin. Teteraploid plants were obtained only from shoots regenerated from explants treated with 125 ?M colchicine and 10 ?M oryzalin. Oryzalin was thus confirmed to be effective at a lower concentration than colchicine.

Article Info

Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 047, Issue 5, Sep 13 - Oct 13, Page 684 - 694 |  PDF |  Page

ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Thidiazuron and Other Plant Bioregulators for Axenic Culture of Siam Cardamom (Amomum krervanh Pierre ex Gagnep.))

ผู้เขียน:ดร.สุรวิช วรรณไกรโรจน์, รองศาสตราจารย์, Wondyifraw Tefera

สื่อสิ่งพิมพ์:pdf

Abstract

Siam cardamom, one of the most common spice and medicinal plants in Southeast Asia, is usually propagated by vegetative means. Identifi cation of suitable plant bioregulators for high shoot proliferation and growth rate is one of the most important steps for an effective micropropagation protocol. The axillary bud from the rhizome of Siam cardamom could be used as an explant for micropropagation. High axillary shoot proliferation (mean = 10.25 shoots per explant) and plant growth rate were attained using a modifi ed Murashige and Skoog (MS) medium with 0.5 mg.L-1 thidiazuron (TDZ) and 2 mg.L-1 imazalil. An MS medium supplemented with 0.75 mg.L-1 TDZ and 3.0 mg.L-1 6-benzyladenine also enhanced shoot multiplication (8.45 shoots per explant). Proliferated shoots successfully elongated and rooted when transferred to the basal MS medium. Inclusion of paclobutrazol to the medium exerted a negative effect on the growth and development of the Siam cardamom.

Article Info

Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 046, Issue 3, May 12 - Jun 12, Page 335 - 345 |  PDF |  Page