Search Result of "Wijit Wonglumsom"

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การประชุมวิชาการ

Simple and Visual Detection of Salmonella using Amino modified Magnetic Nanoparticles

ผู้แต่ง:ImgYortyot Seetang Num, ImgWijit Wonglumsom, ImgDr.Kooranee Tuitemwong, Associate Professor, ImgPravate Tuitemwong,

การประชุมวิชาการ:

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การประชุมวิชาการ

Application of amino-modified magnetic nanoparticles as adsorbents for isolation of DNA from Campylobacter spp.

ผู้แต่ง:

การประชุมวิชาการ:

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ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Occurrence of Arbuscular Mycorrhizal Fungi in Vegetables Grown in Nakornpathom Province, Thailand)

ผู้เขียน:ImgWijit Wonglumsom, ImgNarakorn Surasorn, ImgAmornthep Kochatung

สื่อสิ่งพิมพ์:pdf

Abstract

Arbuscular mycorrhizal colonization in the roots and spore numbers in the rhizosphere of vegetables grown in Nakornpathom province of Thailand were studied. A percentage of fungal infection in the roots was analyzed by a staining method of Phillip and Hayman. A quantity of mycorrhizal spores was determined by a modification of a wet-sieving and decanting technique, and centrifugation method. Spore numbers (mean?SD) of garlic, Chinese kale and broccoli plants were 64.59?28.23, 64.09?36.62 and 45.70?33.01 spores per 100 grams of dry soil, respectively. Three genera of spores were identified from these plants, including Acaulospora, Gigaspora and Glomus with occurrence of individuals ranging from 48.05% to 100%. Internal vesicules and arbuscules of mycorrhizal fungi were observed only in garlic roots for all samples with the infection rates of 28.95?26.75%. There was no relationship between spore numbers and mycorrhizal colonization levels in the garlic plants. An occurrence of mycorrhizal spores in soils for cultivation of broccoli and Chinese kale did not affect the fungal colonization in the roots.

Article Info
Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 040, Issue 5, Jan 06 - Jun 06, Page 82 - 89 |  PDF |  Page 

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ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Continuous Personal Competency Improvement by Remote Education)

ผู้เขียน:ImgPhantip Vattanaviboon, ImgKulnaree Sirisali, ImgSudarat Manochiopinij, ImgWijit Wonglumsom, ImgPairoj Leelahakul, ImgSophon Sirisali

สื่อสิ่งพิมพ์:pdf

Abstract

Quality assurance concept concerns not only non-human factors, but also human competency. In this study, we evaluated two phase competency assessments in hematological techniques from the same group of Thai laboratory staffs with 78% and 75% response rate. Laboratory staffs who participated in our program would receive informed consent forms and the assessment materials via mail. Cell and blood smear identifications and quality control process were assessed in the first phase. Case scenarios derived from improper answers of the first phase were tested in the second phase. The answers were assessed and graded as 3 levels (3 = very good, 2 = good and 1 = fair) and compared between both phases. The results showed an average grade of 2.27 ? 0.55 and 2.31 ? 0.29 for the first and second phase. The distribution of participant’s grade in the first and second phase were 42.4%, 47.2%, 10.4% and 30.0%, 68.8%, 1.2% for grade 3, 2, 1, respectively. This result implies the improvement of laboratory personal competency, especially those who earned a low grade demonstrated a dramatic improved (p<0.01).

Article Info
Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 040, Issue 5, Jan 06 - Jun 06, Page 9 - 12 |  PDF |  Page 

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ที่มา:วิทยาสารเกษตรศาสตร์ สาขา สังคมศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Enhancement of Medical Technologist Competency by the People?Education Cycle)

ผู้เขียน:ImgKulnaree Sirisali, ImgSudarat Manochiopinij, ImgPairoj Leelahakul, ImgPhanthip Vattanaviboon, ImgSophon Sirisali, ImgWijit Wonglumsom

สื่อสิ่งพิมพ์:pdf

Abstract

A competent medical technologist is one required component of clinical laboratory quality. For sustainable competency, continuing education is the empowering mean. In this study, we used both ?Traditional Education? and ?Mobile Education? as systems of continuing education, and the effectiveness of the two systems were assessed. Two hundreds and ninety eight medical technologists entered the ?Traditional Education? system, whereas 290 and 483 medical technologists participated in the ?Mobile Education? system in the year 2006 and 2007 respectively. The ?Traditional Education? was offered only in Bangkok, whereas the ?Mobile Education? was carried in both provinces and Bangkok. Outcome of both systems was evaluated through participant?s competency. The successful index was the ability of presenting a self-created project based on the laboratory problem in a national contest. Comparing of the two systems, the ?Mobile Education? system was more efficient. A 3?time of created projects was obtained. This dramatic increase occurred in the year 2007. Surprisingly, 2 small laboratories as community hospital ones were also able to demonstrate their competency. Results showed that our chosen continuing education system especially the ?Mobile Education? reached the intended goal.

Article Info
Kasetsart Journal of Social Sciences -- formerly Kasetsart Journal (Social Sciences), Volume 029, Issue 2, May 08 - Aug 08, Page 177 - 181 |  PDF |  Page 

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ที่มา:วิทยาสารเกษตรศาสตร์ สาขา สังคมศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Evidence-based Learning as a Tool of Competency Improvement for the Phlebotomist)

ผู้เขียน:ImgWijit Wonglumsom, ImgSudarat Manochiopinij, ImgSophon Sirisali, ImgPhantip Vattanaviboon, ImgKulnaree Sirisali

สื่อสิ่งพิมพ์:pdf

Abstract

Nowadays, clinical laboratory service has an important role in patient care management. A quality test result must be obtained, but this can be affected by many factors. The correct process for specimen acquisition is very necessary and must be done by competent professional personnel. The objective of this study was to improve the competency of blood collection by phlebotomists through evidence-based learning. Evidence-based information gathering from nurses, medical technologists and clinical laboratory assistants was analyzed and synthesized to use as teaching material. Subjects were divided into two groups. The results showed that the trained groups of the ?lecture? type and the ?evidence-based? type had different levels of improvement, with the more competent group being the evidence-based one. Five out of eight assessed parameters showed significant improvement. The overall score was increased from 49.79 to 73.08 percent In addition, the evidence-based group showed their improved competency by the ability to improve the venipuncture process from 76.59 to 92.31 percent and the time to perform venipuncture per patient was reduced by 43 seconds.

Article Info
Kasetsart Journal of Social Sciences -- formerly Kasetsart Journal (Social Sciences), Volume 032, Issue 1, Jan 11 - Apr 11, Page 134 - 139 |  PDF |  Page 

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ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : External Quality Assessment in Isolationand Identification of Bacteria)

ผู้เขียน:ImgWijit Wonglumsom, ImgChaniya Leepiyasakulchai, ImgWacharee Tiyasuttipan

สื่อสิ่งพิมพ์:pdf

Abstract

External quality assessment for evaluation of individual competency in isolation and identification of bacteria for clinical microbiological laboratory was studied. Lyophilized or freezedried bacteria were prepared and a panel of lyophilized bacteria was shipped once a year. The first set consisted of two mixed cultures and one pure culture. Forty-three laboratories participated in this first set. The second set provided three ampoules of lyophilized mixed bacteria. Thirty-eight laboratories participated in this second set. Fifteen (34.9%) laboratories and 21 (55.3%) laboratories were correctly reported for the first and second proficiency sets, respectively. Of 33 laboratories participated in both trials, fifteen (46%) laboratories showed performance improvement. A meeting concerning on quality assurance and good laboratory practices in clinical microbiology was also provided for participating laboratories before shipment of the second panel. This pilot scheme showed a high possibility of establishing the External quality assessment scheme in bacterial isolation and identification for continuous improvement of quality assurance.

Article Info
Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 042, Issue 5, Dec 99 - Dec 99, Page 219 - 224 |  PDF |  Page 

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ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Evaluation of Membrane Fragments Extracted from Escherichia coli and Pseudomonas aeruginosa on Campylobacter jejuni Growth under Normal Atmosphere)

ผู้เขียน:ImgPattara Wongjaroen, ImgKanokwan Kittiniyom, ImgPotjanee Srimanote, ImgWacharee Tiyasuttipan, ImgWijit Wonglumsom

สื่อสิ่งพิมพ์:pdf

Abstract

Study on extraction of membrane fragments from Escherichia coli and Psuedomonas aeruginosa were determined for using to support Campylobacter cultivation under normal atmosphere. Crude membrane fragments were extracted from 45 strains of E. coli and 44 strains of P. aeruginosa. Strains that provided highest efficiency of oxygen reduction were selected to purify membrane fragments by French pressure cell and ultracentrifugation. The purified membrane fragments were characterized and investigated for supporting Campylobacter jejuni growth in Mueller-Hinton broth. The broth supplemented with and without membrane fragments was incubated under normal atmosphere at 37?C or 42?C. Campylobacter growth in the broth containing purified membrane fragments was initially observed within 6 to 12 hours of incubation while the media without supplemented with membrane fragments showed no growth of the bacteria. Therefore, oxygen reducing membrane fragments prepared from the selected strains could support Campylobacter cultivation under normal atmosphere.

Article Info
Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 042, Issue 5, Dec 99 - Dec 99, Page 213 - 218 |  PDF |  Page 

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งานวิจัย

การพัฒนาชุดทดสอบรวดเร็วด้วยอนุภาคฟลูออเรสเซนต์ซิลิการ่วมกับเฟอร์โรแมกเนติกสำหรับ Campylobacter spp. และ Salmonella spp. (2016)

หัวหน้าโครงการ:ประเวทย์ ตุ้ยเต็มวงศ์

ผู้ร่วมโครงการ:Imgดร.ฆรณี ตุ้ยเต็มวงศ์, รองศาสตราจารย์, Imgประเวทย์ ตุ้ยเต็มวงศ์, Imgยอดยศ ศรีตังนันท์, Imgวิจิตร วงศ์ล่ำซำ, Imgอารี ทัตติยพงศ์

แหล่งทุน:สำนักงานคณะกรรมการวิจัยแห่งชาติ (วช) และ สำนักงานกองทุนสนับสนุนการวิจัย (สกว)

ผลลัพธ์:วารสาร (2)

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Researcher

ดร. ฆรณี ตุ้ยเต็มวงศ์, รองศาสตราจารย์

ที่ทำงาน:ภาควิชาจุลชีววิทยา คณะวิทยาศาสตร์ บางเขน

สาขาที่สนใจ:Food Microbiology, Rapid methods & Automation in Microbiology

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