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ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Analysis of Intersectional Hybrids of Dendrobium by RAPD Technique)

ผู้เขียน:ImgSirilak Inthawong, ImgWeenun Bundithya, ImgNuttha Kuanprasert, ImgPimchai Apavatjrut

สื่อสิ่งพิมพ์:pdf

Abstract

Thirty interspecific hybridizations of inter- or intra-section of Dendrobium were made. Five crosses showed good compatibility and could yield hybrids as follows: 2 intersectional hybridizations, 1) section Phalaenanthe: Den. phalaenopsis (D017) ? section Formosae: Den. draconis (D022) and 2) section Dendrobium: Den. finlayanum (D030) ? section Formosae: Den. cariniferum (D018); and 3 intrasectional hybridizations, 1) section Dendrobium: D030 ? Den. nobile (D031), 2) section Formosae: Den. trigonopus (D037) ? D022, and 3) D037 ? Den. infundibulum (D034). The greatest number of plantlets (1,250 plantlets) was obtained from cross D037 ? D022 whereas the least number (3 plantlets) was found in cross D037 ? D034. After transplanting for 6 months, survival rate of D037 ? D034 was 100% whereas no survival plant was found in cross D030 ? D018. Parents and hybrids of 5 crosses were analyzed by RAPD using 21 decamer primers and the number of primers generating polymorphic patterns of each cross varied. RAPD markers were able to differentiate parents in inter- and intra-sectional crosses and these markers could confirm the interspecific hybrids.

Article Info
Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 040, Issue 2, Apr 06 - Jun 06, Page 456 - 461 |  PDF |  Page