Search Result of "WONGURAI, A."

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ที่มา:วารสาร คณะสัตวแพทยศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : PRODUCTION OF POLYCLONAL ANTIBODY AGAINST FUMONISIN B1 AND DETECTION BY ELISA IN THAILAND)

ผู้เขียน:ImgCHINAPHUTI, A., ImgWONGURAI, A.

สื่อสิ่งพิมพ์:pdf

Abstract



Article Info
วารสาร คณะสัตวแพทยศาสตร์(Journal of Kasetsart Veterinarians), Volume 016, Issue Congress1, Dec 99 - Dec 99, Page 44 - 44 |  PDF |  Page 

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ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Production of In-house ELISA Test Kit for Detection of Aflatoxin in Agricultural Commodities and Their Validations)

ผู้เขียน:ImgAmara Chinaphuti, Imgชวเลิศ ตรีกรุณาสวัสดิ์, ImgArunsri Wongurai, Imgศุภรัตน์ โฆษิตเจริญกุล

สื่อสิ่งพิมพ์:pdf

Abstract

A specific microtest plate enzyme immunoassay was developed in Thailand for the rapid detection of aflatoxin B1 (AFB1) using Polyclonal antibody against AFB1 . Percentage of the antibody cross reaction to AFB1, AFB2, AFG1 and AFG2 were 100, 21.4, 25.0 and 2.5% respectively. AFB1-HRP (Horseradish Peroxidase) conjugate was used as direct competitive test for AFB1 detection. The DOA- Aflatoxin ELISA Test Kit was produced for detection of both qualitative and quantitative results within 1 hr after sample preparation with the minimum of 0. 4 ppb per assay. There was no significant difference in detection efficiency of this test kit compared to traditional methods of minicolumn, TLC and HPLC. Moreover, the kit obtained 82-100% AFB1 recovery from the spike samples. Validation for the analysis of AFB1 in sample matrices was also tested using peanut extract which had the virtually identical curves to the standard. Detection efficiency of DOA–Aflatoxin ELISA test kit was not different from those imported from the USA and Italy.

Article Info
Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 036, Issue 2, Apr 02 - Jun 02, Page 179 - 186 |  PDF |  Page