Search Result of "Utit Trenuntawan"

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ที่มา:วารสาร คณะสัตวแพทยศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Comparison of Serological Tests for Antibody Detection Against Brucella melitensis Infection in Goats)

ผู้เขียน:ImgMonaya Ekgatat, ImgReka Kanitpun, ImgPittaya Kunchit, ImgWorawan Arampong, ImgSornsak Raksajit, ImgSuree Thammasart, ImgUtit Trenuntawan, ImgPhairuch Tumcha, ImgSurapong Wongkasemjit

สื่อสิ่งพิมพ์:pdf

Abstract

Four serological assays, including Rose Bengal test (RBT), modified RBT (mRBT), complement fixation test (CET) and indirect enzymelinked immunosorbent assay (I-ELISA), were evaluated for their efficiency on diagnosis of brucellosis in goats. Serum to antigen ratio, used for RBT and MRBT, which allowed for optimal sensitivity, was 1:1 and 3:1, respectively. CET was performed using microtechnique combined with cold fixation protocol. I-ELISA was performed using conjugated protein G and smooth lipopolysaccharide (sLPS) as antigen with percent positivity (PP) cutoff at 30. Specimens tested included 1,317 goat sera, of which 127 were positive and 1,190 were negative as confirmed by isolation of Brucella melitensis. At 95% of confidence, diagnostic sensitivity (DSe) was 99.2% by RBT,100 % by mRBT, 95.3%by CFT and 99.2% by I-ELISA, while their diagnostic specificity (DSp) was 100% by RBT, mRBT and CFT , and 99.9% by I-ELISA,Comparison of each assay with B. melitensis isolation showed that accuracy (Ac) , kappa (K) value , positive predictive value (PPV) and negative predictive value (NPV) were high. The results of this study showed that RBT, mRBT and I-ELISA were sensitive, specific and suitable for screening , while CFT was suitable for confirmation of Brucella spp. infection in goats. Further study on herd prevalence of brucellosis is needed for the dvelopment of a long-term national prevention and control program of brucellosis in goats.

Article Info
วารสาร คณะสัตวแพทยศาสตร์(Journal of Kasetsart Veterinarians), Volume 020, Issue 1, Jan 10 - Apr 10, Page 19 - 26 |  PDF |  Page 

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ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Indirect Enzyme-Linked Immunosorbent Assay Test Kit Development for Specific Antibody Detection Against Brucella abortus in Cattle)

ผู้เขียน:ImgMonaya Ekgatat, ImgSuree Thammasart, ImgReka Kanitpun, ImgSurapong Wongkasemjit, ImgChokchai Nokdhes, ImgUtit Trenuntawan

สื่อสิ่งพิมพ์:pdf

Abstract

The smooth lipopolysaccharide (SLPS) antigen, prepared from Brucella abortus strain 99 was used in an indirect Enzyme-Linked Immunosorbent Assay (iELISA). The data were expressed as optical density and generated using computer software. The positive results were accepted at the cut-off values of ? 40 percent positivity (PP). The strong positive serum control (C++), weak positive serum control (C+), negative serum control (C-), and conjugate control (Cc) were used to optimize the test kit. The iELISA was optimization and standardization for antigen concentration, test material dilution and detection system dilution by checkerboard titration. The statistic evaluation using data from sera from 317 infected and 5,300 non-infected cattle revealed that the sensitivity (Se) is 99.369 (98.497-100.0)%, specificity (Sp) is 99.887 (99.796-99.977)%, accuracy (Ac) is 99.858% and Kappa value is 0.987. The iELISA test kit was subsequently validated by testing 9,877 field serum samples submitted to laboratory. The complement fixation test was used as gold standard. The results revealed that the relative Se is 99.026 (97.929-100.0)%, relative Sp is 98.547 (98.308-98.787)%, relative Ac is 98.562% and Kappa value is 0.804 when cattle sera were tested. The components of this iELISA test kit consisted of antigen, control sera, and essential diluents for 800 tests. This test showed high sensitivity, specificity, and accuracy and could be performed in mass. Therefore, this iELISA test kit might be an appropriate tool for brucellosis control in Thailand.

Article Info
Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 042, Issue 5, Dec 99 - Dec 99, Page 95 - 100 |  PDF |  Page