ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Phase Characterization of TiO2 Powder by XRD and TEM)

ผู้เขียน:เขมฤทัย ถามะพัฒน์

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Abstract

In this study, the commercial TiO2 nanopowder and micropowder of anatase phase and rutile phase have been characterized by x-ray diffraction (XRD) and transmission electron microscopy (TEM). XRD patterns of nano-TiO2 in rutile and anatase phases exhibit broad peaks whereas both phases of micro-TiO2 demonstrate very sharp peaks. TEM images show that the grain size of TiO2 micropowders and TiO2 nanopowders are 0.3-0.7 ?m and 10 nm, respectively. The selected-area electron diffraction patterns of TiO2 nanopowders in rutile and anatase phases are consistent with XRD results.

Article Info

Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 042, Issue 5, Dec 99 - Dec 99, Page 357 - 361 |  PDF |  Page

ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Effects of Extender and Storage Time on Motility and Ultrastructure of Cooled-Preserved Boar Spermatozoa)

ผู้เขียน:Duangjai Boonkusol, Kulnasan Saikhun, Poonsuk Ratanaphumma

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Abstract

Artificial insemination (AI) using fresh diluted semen is used worldwide in the porcine industry. Viability of spermatozoa in diluted semen depends on several factors, such as the interaction with the type of extender and storage duration. The aim of this study was to evaluate the effects of extenders and storage time on the motility and ultrastructure of cooled-preserved boar spermatozoa. Semen samples were collected, diluted in BTS, Merck III or Androhep and stored at 15?C for 0, 1, 3, 5 and 7 d. The samples were warmed (37?C) and spermatozoa were evaluated for motility using light microscopy and ultrastructure using scanning (SEM) and transmission (TEM) electron microscopy. The results showed that motility did not differ significantly (P>0.05) among extenders at days 0 to 3, while Androhep showed a significantly (P<0.05) higher percentage of motility than BTS and Merck III from days 5 to 7. At day 7 of semen storage, plasma and acrosomal membrane damage were observed, as revealed by SEM and TEM. These findings suggest that the type of semen extender and cooled-preserved time affected motility and caused structural damage to boar spermatozoa, especially in the plasma and acrosomal membrane.

Article Info

Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 044, Issue 4, Jul 10 - Aug 10, Page 582 - 589 |  PDF |  Page