Search Result of "Sureerat Yenchon"

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ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Sonication-Assisted Agrobacterium-mediated Gene Transformation of Oil Palm Secondary Somatic Embryo)

ผู้เขียน:ImgSureerat Yenchon, ImgSompong Te-chato



The infl uence was investigated of the hygromycin concentration on the survival rate of oil palm secondary somatic embryos (SSEs) and sonication-assisted Agrobacterium-mediated transformation (SAAT). The results showed that 20 mg.L-1 hygromycin could completely inhibit growth of SSEs, suitable for selection of transformed SSEs. The transient ?-glucuronidase (gus) expression was affected by the different period of SAAT which was signifi cantly enhanced by increasing the period of SAAT. However, the longer exposure to SAAT caused a decrement in the frequency of plantlet regeneration. The highest transformation effi ciency was obtained when the SSEs were sonicated for 5 min. Polymerase chain reaction and Southern blot hybridization analysis confi rmed the presence of the reporter genes in the transformed plantlet genome.

Article Info
Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 049, Issue 3, May 15 - Jun 15, Page 319 - 326 |  PDF |  Page