Search Result of "S. Suputitada"

About 1 results

ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์

หัวเรื่อง:การชักนำให้เกิดการกลายในข้าวโดยวิธีเพาะเลี้ยงเนื้อเยื่อ ตอนที่ 1

ผู้เขียน:ImgP. Pongtongkam, ImgP. Ratisoontorn, ImgS. Suputitada, ImgS. Peyachoknagul, ImgL. Ngernsiri, ImgB. Silayoi, ImgP. Rodrangboon, ImgK. Klakhaeng, ImgR. Senghaphan



Seeds of Basmati 370 rice variety were cultured on the modified MS medium supplemented with various concentrations of 2,4-D, NAA and kinetin. The suitable callus induction medium was the modified MS medium containign I mg/l 2,4-D, 2 mg/l NAA and 1 mg/l kinetin. Calli from this medium were divided into two groups, one was 3 week old calli (non subcultured calli) and the other was 6 week old calli (subcultured calli). Both groups seemed to grow and develop into plantles quite well on the modified MS medium supplemented with 2 mg/l kinetin. However the percentage of plantles induction from 3 week old calli was better. Calli of both groups were irradiated with different doses of gamma radiation and were cultured on the regenerated medium. The 3 week old calli were better than 6 week old calli in regenerating into plantles.

Article Info
Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 028, Issue 3, Jul 94 - Sep 94, Page 381 - 389 |  PDF |  Page