 ผลงานตีพิมพ์ในวารสารวิชาการCatfish species identification using lab-on-chip PCR-RFLPผู้แต่ง: Wong, L.L., Peatman, E., Kelly, L., Kucuktas, H.,  Dr.Uthairat Na-Nakorn, Professor , Liu, Z., วารสาร:  
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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Molecular Identification of Cycas by Restriction Fragment Length Polymorphism (RFLP) and Random Amplified Polymorphic DNA (RAPD)) ผู้เขียน:พัทธมน แสงอินทร์, นางอมรา ทองปาน, รองศาสตราจารย์ , Anders J. Lindstrom, ดร.มิ่งขวัญ มิ่งเมือง, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractRAPD and RFLP were used to identify nineteen species of Cycas. Ten species of these Cycas namely C. chamaoensis, C. macrocarpa, C. pectinata, C. clivicola, C. pranburiensis, C. litoralis, C. tansachana, C. siamensis, C. nongnoochiae and C. simplicipinna are locally found in Thailand while the nine remaining species of C. seemannii, C. wadei, C. bougainvilleana, C. chevalieri, C. diannanensis, C. nathorstii, C. edentata, C. parvulus and C. micholitzii are from several countries around the world but collectively planted at Nong Nooch Tropical Botanical Garden. In the RAPD study, twenty random primers were screened to amplify the genomic DNA of nineteen species of Cycas. Only five primers, i.e., OPB-1, OPB-8, OPB-14, OPB-15 and OPB-17 of ten nucleotides long were found to give polymorphic DNA patterns. These eighty-seven bands of Cycas DNA at the size of 0.35 -2.5 kb could be used to indicate the differences of these Cycas. As for RFLP, three probes were synthesized from 5S rRNA gene, 5S rRNA repeat unit gene of C. clivicola and 18S rRNA gene of C. pranburiensis. The probes were hybridized with the genomic DNA of Cycas which had been digested with restriction enzymes BamHI, EcoRI and DraI. The phylogenetic trees were constructed based on their similarity index derived from DNA polymorphism of RAPD and RFLP separately. The RAPD data classified nineteen species of Cycas into two major groups which mostly corresponded to their geographic origins, i.e., one group of Thailand origin and another of other countries. However, the RFLP data gave a different set of grouping showing more to their morphological characteristics but less on their geographic origins. |
ที่มา:วิทยาศาสตร์เกษตรหัวเรื่อง:การวิเคราะห์สายพันธุ์เห็ดฟางด้วยวิธี ITS-PCR-RFLP |
ที่มา:การประชุมทางวิชาการของมหาวิทยาลัยเกษตรศาตร์ ครั้งที่ 48หัวเรื่อง:การแยกชนิสัตว์ในสกุล Varanus spp. ที่พบในประเทศไทยดยเทคนิค Polymerase Chain reaction-Restriction Fragment Length Polymorphophism (PCR-RFLP) |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : In silico PCR-RFLP of Bacillus Species: Problem-Based Case of Teaching Bioinformatics) ผู้เขียน:Ekachai Chukeatirote, Komsit Wisitrasamewong, Jedsadapa Jongmahasavat สื่อสิ่งพิมพ์:pdf AbstractBioinformatics is a multi-disciplinary subject that encompasses a wide range of fields including; genomics, biotechnology, information technology, algorithms and statistics. As a result, it is important to establish a skeletal set of courses capable of providing both a general and a detailed background on all aspects of bioinformatics. In this study, a problem-based project entitled “in silico PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) of Bacillus species” was assigned to students. The project aimed to cover the background of genetic information retrieval, multiple sequence alignment, phylogenetic analysis and in silico PCR-RFLP. Initially, the 16S rRNA genes of 34 Bacillus species were retrieved from the NCBI database. The sequence data obtained were then analyzed in terms of their similarities and variation using the ClustalW software. Subsequently, the phylogenetic tree was constructed to investigate their evolutionary relationship. The results indicated that these Bacillus species could be grouped into different clades. In addition, in silico PCR-RFLP was performed to introduce students to the principles of molecular taxonomy. |
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