 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Expression of Fatty Acid Desaturase Enzymes from Cyanobacterium Spirulina platensis in Yeast Saccharomyces cerevisiae) ผู้เขียน: Peerada Prommeenate, Pavinee Kurdrit, Matura Sirijuntarut, Apiradee Hongsthong สื่อสิ่งพิมพ์:pdf AbstractSpirulina sp. is the cyanobacterium that has an ability to produce ?-linolenic acid or GLA at high level (1-1.5% of dry weight) which could be used as a new source of GLA production. The biosynthesis of GLA in Spirulina sp. involved with three enzymes, ?9, ?12 and ?6 desaturase which encoded by gene desC, desA and desD, respectively. The desaturase genes from Spirulina that involve in GLA biosynthesis from cyanobacteria were studied by gene cloning and transforming into yeast, Saccharomyces cerevisiae. Gas Chromatography has been used to follow the activity of desaturase enzymes in yeast cells. The results indicated that both ?12 and ?6 desaturase genes from Spirulina
platenesis can functionally co-expressed in the heterologous yeast system. The final product, GLA has been detected under the condition described here. |