Search Result of "Pattara Wongjaroen"

About 1 results

ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Evaluation of Membrane Fragments Extracted from Escherichia coli and Pseudomonas aeruginosa on Campylobacter jejuni Growth under Normal Atmosphere)

ผู้เขียน:ImgPattara Wongjaroen, ImgKanokwan Kittiniyom, ImgPotjanee Srimanote, ImgWacharee Tiyasuttipan, ImgWijit Wonglumsom



Study on extraction of membrane fragments from Escherichia coli and Psuedomonas aeruginosa were determined for using to support Campylobacter cultivation under normal atmosphere. Crude membrane fragments were extracted from 45 strains of E. coli and 44 strains of P. aeruginosa. Strains that provided highest efficiency of oxygen reduction were selected to purify membrane fragments by French pressure cell and ultracentrifugation. The purified membrane fragments were characterized and investigated for supporting Campylobacter jejuni growth in Mueller-Hinton broth. The broth supplemented with and without membrane fragments was incubated under normal atmosphere at 37?C or 42?C. Campylobacter growth in the broth containing purified membrane fragments was initially observed within 6 to 12 hours of incubation while the media without supplemented with membrane fragments showed no growth of the bacteria. Therefore, oxygen reducing membrane fragments prepared from the selected strains could support Campylobacter cultivation under normal atmosphere.

Article Info
Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 042, Issue 5, Dec 99 - Dec 99, Page 213 - 218 |  PDF |  Page