 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Callus induction and Growth of Zea mays. L var. saccharata (Super sweet corn) through Tissue Culture ) ผู้เขียน: Tanong Pronpradupkait, Paiboolya Gavinlertvatana สื่อสิ่งพิมพ์:pdf AbstractCallus induction of Zea mays var. saccharata (super sweet) on Murashige and Skoog (1962) medium containing 1 or 3 mg/1 2, 4-D was found. Tissue explants from root tip and mesocotyl were suitable for callus formation. The induction and growth of callus was better in medium containing 1 mg/1 2, 4-D and 10% coconut milk than medium containing 3 or mg/1 2, 4-D and coconut milk. Callus from root tip which was cultured in medium containing 1 or mg/1 2, 4-D grew at a greater extent under condition of light than in the dark. In contrast, callus from mesocotyl which was cultured in medium containing 1 mg/1 2, 4-D grew better under condition of dark than in the light. Nevertheless, callus grew better in the dark in medium which was supplemented with %coconut milk. The increasing percentage of callus growth was greater in medium containing 3 mg/1 2,4-D and 10% coconut milk or in coconut milk free-medium. Root formation occurred in callus which was cultured in medium containing 1 or 3 mg/1 2,4-D than in medium containing 1 mg/1 2,4-D than in medium containing 3 mg/1 2,4-D. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Propagation of Amaryllis through Tissue Culture) ผู้เขียน:Salux Phunsiri, Paiboolya Gavinlertvatana, Preedee Akavipat สื่อสิ่งพิมพ์:pdf AbstractBulb-scale sections of amaryllis (Hippestrum hybridum), 8 hybrids and one native variety, were cultured on modified Murashige and Skoog basal medium supplemented with 2,4 dichlorophenoxyacetic acid (2, 4-D) at 0.3 or 3 ppm combined with 6-furfurylamino purine (kinetin) at 0 or 0.3 ppm. Five varieties, Caroussel, Excelsior, Scarlet Leader, Rembrandt and Rilona, were able to regenerate bulblets. Excelsior tissues formed callus 90% and regenerate bulblet 50%. Both Rilona and Caroussel tissues formed callus 70% and bulblet 30%. Regeneration of bulblet in four varieties took 60-70 days but Caroussel variety took 32 days. |