Search Result of "P. Sripichit"

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ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์

หัวเรื่อง:การเพาะเลี้ยงอับเรณูของข้าวลูกผสม (ขาวดอกมะลิ 105/สไคบอนเนท)

ผู้เขียน:ImgP. Pongtongkam, ImgR. Lervichai, ImgS. Apisitiwanich, ImgS. Peyachoknagul, ImgK. Klakhaeng, ImgP. Sripichit, ImgN. singburaudom, ImgS. Fungfupong, ImgP. Srinives

สื่อสิ่งพิมพ์:pdf

Abstract

The anthers of F1 hybrid rice (Khao Dawk Mali 105/Skybonnet) were cultured on the various callus induction media. It was found that a suitable medium for callus induction was N6 containing 2 mg/l 2,4-D, 1 mg/l kinetin, 500 mg/l casein hydrolysate and 50 g/l sucrose. The plantlets could be produced from callus on the MS medium supplemented with 2 mg/l BAP, 1 mg/l kinetin, 0.2 mg/l NAA, 300 mg/l casein hydrolysate, 15% coconut water and 20 g/l sucrose. After transferring these plantlets onto the MS medium containing 1 g/l yeast extract, 15% coconut water, 3 mg/l BAP and 0.01% colchicines for 5 days, about 60% of the doubled haploid plants were recovered.

Article Info
Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 029, Issue 2, Apr 95 - Jun 95, Page 158 - 166 |  PDF |  Page 

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ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Effects of L-lysine on Callus Formation , Plant Regeneration and Flowering of Thai Rice c.v. KDML 105)

ผู้เขียน:Imgนายประดิษฐ์ พงศ์ทองคำ, ศาสตราจารย์, Imgสุรินทร์ ปิยะโชคณากุล, ImgPrapa Sripichit, Imgนางอมรา ทองปาน, รองศาสตราจารย์, Imgกาญจนา กล้าแข็ง, ImgSaowanee Ketsagul, ImgKanokporn Lertsirirungson

สื่อสิ่งพิมพ์:pdf

Abstract

Seed-cultured of KDML 105 on Murashige and Skoog (MS) medium supplemented with 2 mg/ l 2,4–D , 1 g/l L-proline , 1 g/l casein hydrolysate and 20 ?M L-lysine produced the highest average percentage of callus formation (95.16%). Regeneration of the calli into plantlets could be best achieved at the average of 63.87 % in the MS medium containing 1 mg/l kinetin, 1 g/l L–proline, 300 mg/l casein hydrolysate and 20 ?M L-lysine. Plantlets were cultured in the MS medium containing 3 mg/l BAP, 3 g/l activated charcoal and 20 ?M L-lysine before transferring to pots under greenhouse condition. Two plants out of 80 plantlets reached the flowering stage under long photoperiod and produced only 6 seeds altogether. The seeds were subsequently planted for 2 generations and DNA fingerprints of these plants were determined using sequence related amplified polymorphism (SRAP) technique. All 6 clones of the L-lysine-treated plants gave different DNA patterns from that of the original KDML105 suggesting some effects of L-lysine on their genetic variation.

Article Info
Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 038, Issue 2, Apr 04 - Jun 04, Page 190 - 195 |  PDF |  Page