 ผลงานตีพิมพ์ในวารสารวิชาการIncidence and molecular diversity of poleroviruses infecting cucurbit crops and weed plants in Thailandผู้แต่ง: Cheewachaiwit, S., Warin, N., Phuangrat, B., Rukpratanporn, S., Gajanandana, O., Balatero, C.H.,  Ms.Orawan Chatchawankanphanich , วารสาร:  
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ผลงานตีพิมพ์ในวารสารวิชาการEthylene and pollination decrease transcript abundance of an ethylene receptor gene in Dendrobium petalsผู้แต่ง:Thongkum, M., Burns, P., Bhunchoth, A., Warin, N., Ms.Orawan Chatchawankanphanich, Van Doorn, W.G., วารสาร: |
ผลงานตีพิมพ์ในวารสารวิชาการDetection of quantitative trait loci for mungbean yellow mosaic India virus (MYMIV) resistance in mungbean (Vigna radiata (L.) Wilczek) in India and Pakistanผู้แต่ง:Dr.Prakit Somta, Associate Professor , รัตนากร กฤษณาชาญดี, Orawan Chatchawankanphanich, Khalid P. Akhtar, Tariq Mahmud Shah, Ramakrishnan M. Nair, Tejinderjit S. Bains, Asmita Sirari, Livinder Kaur, Dr.Peerasak Srinives, Professor , วารสาร:  |
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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Occurrence of Non-infectious Phyllody Disease of Strawberry in Thailand) ผู้เขียน:นางสาวอรวรรณ (ยกเลิก) ชัชวาลย์การพาณิชย์ (ยกเลิก), นางสุวรรณา กลัดพันธุ์ , Narongchai Pipatthanvong, Kruapan Kittipakorn, Wanphen Srithongchai สื่อสิ่งพิมพ์:pdf AbstractStrawberries showing phyllody symptom on fruits has been observed in No.50 cultivar grown in northern region of Thailand since 1996. The symptomatic strawberries produce very poor fruit quality and unmarketable. The disease was epidemic in many strawberry growing areas. The abnormality symptom is similar to the green petal disease which caused by phytoplasma. Several methods including electron microscopy, serological methods, and PCR were used to detect phytoplasma in symptomatic strawberries. All methods gave negative results and phytoplasma could not be detected. In addition, the disease was proved not transmitted to healthy strawberry and periwinkle by grafting and dodder transmission. Evidently, this phyllody disease of strawberry is not caused by phytoplasma. It is possibly caused by physiological disorder and is called non-infectious phyllody disease that was previously found in strawberry in Italy. |
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หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Interactive Effect of Ralstonia solanacearum on Lethal Wilt of Chili Caused by Phytophthora capsici) ผู้เขียน:ดร.ชลิดา เล็กสมบูรณ์, รองศาสตราจารย์ , นางสาวอรวรรณ (ยกเลิก) ชัชวาลย์การพาณิชย์ (ยกเลิก), Sirilak Suwanwongse สื่อสิ่งพิมพ์:pdf AbstractRalstonia solanacearum and Phytophthora capsici are important soil-borne pathogens associated with lethal wilt symptoms on chili. Both pathogens were found in the same fi eld in Thailand and caused serious disease in north Thailand. The local strains RS1/3 of R. solanacearum (RS) and PC4-1 of P. capsici (PC) were used for investigation of the interactive effect of R. solanacearum on the lethal wilt of chili caused by P. capsici. Chili cultivar CA365 (spur chili), Thaevee60 (hot chili) and commercial cultivar Sabun Nga (spur chili) were inoculated with PC4-1 or RS1/3 alone and by co-inoculation or sequential inoculation. In sequential inoculation, plants were inoculated with RS1/3 prior to PC4-1 by 3, 5 and 7 d. The results showed that PC4-1 was more aggressive than RS1/3 in all tested cultivars, and plants inoculated with both pathogens or PC4-1 alone had signifi cantly more mortality in a shorter time. Plants which were inoculated with both pathogens or PC4-1 alone had total plant death at the same time, while with RS1/3 this occurred prior to PC4-1 by 5 and 7 d (13 d after RS1/3 inoculation). The results also showed that RS had no effect on plant death in the CA365 cultivar (susceptible to PC) when inoculated with RS1/3 prior to PC4-1 by 5 and 7 d. Different results showed in the Thaevee60 cultivar which was moderately resistant to PC and it was found that plants inoculated with RS1/3 prior to PC4-1 by 5 d had total plant death in a shorter time than when there was concomitant inoculation with both pathogens. Treatment of PC alone did not kill all plants whereas treatment of PC and RS concomitantly and of RS prior to PC by 5 d produced total plant death 27 and 24 d after RS inoculation. In the treatment of RS prior to PC by 7 d, all plants had died 24 d after RS inoculation whereas the application of PC alone or of PC and RS concomitantly did not kill all plants. This study indicated that RS enhanced the infection of the moderately resistant cultivar Thaevee60 by PC. In sequential inoculation, total plant death was apparent in the treatment of RS prior to PC by 3 d taking a longer period than in the treatment of RS and PC inoculated concomitantly. There was apparent antagonism between the two pathogens. When PC and RS were paired on a growth medium of potato dextrose agar, the mycelial growth of PC grown alone was signifi cantly different from the mycelial growth of PC grown together with RS. These results were found in all 11 tested strains of RS. |
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หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Characterization of the Sugar Utilization Gene polS from Ralstonia solanacearum) ผู้เขียน:ดวงแข กาญจนโสภา, นางสาวอรวรรณ (ยกเลิก) ชัชวาลย์การพาณิชย์ (ยกเลิก), ดร.ศรีเมฆ ชาวโพงพาง, ผู้ช่วยศาสตราจารย์ , ดร.วิชัย โฆสิตรัตน, รองศาสตราจารย์ , ดร.นิพนธ์ ทวีชัย, ศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractThe gene coding for sorbitol dehydrogenase (polS) from Ralstonia solanacearum strain TO 264 biovar 3 was cloned, sequenced, and compared to homologous sequences from the other bacteria. The result showed that the sorbitol dehydrogenase gene from R. solanacearum strain TO264 displayed the highest similarity to that of R. solanacearum GMI1000 with 99.6% amino acid similarity and the least similarity to sequence of Pseudomonas syringae pv. syringae with 56% amino acid similarity. Phylogenetic analysis of polS showed the Burkholderia cepacia sequence to join as sister to the R. solanacearum TO264 pair. Analysis of the deduced amino acid sequence revealed homology to enzymes of the short-chain dehydrogenase/reductase protein family. The eight amino acid residues were conserved in most of these proteins. These residues included the almost invariant tyrosine (Y) and lysine (K) residues of consensus sequence Y-X-X-X-K, which were essential for catalysis and were located in the active site in C-terminal whereas the glycine (G) residues of the G-X-X-X-G-X-G segment were characteristic of the NAD+ binding domain in the N-terminal region. The 771 bp of polS gene from R. solanacearum TO264 was subcloned into expression pGEX-2T vector. The polS ORF encoded a protein consisting 256 amino acid residues with estimated molecular mass of 27 kDa by SDS-PAGE analysis. |
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