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 การประชุมวิชาการProduction and composition of sulfated polysaccharides as physiological active substance from marine algae of Thailandผู้แต่ง: Dr.Anong Chirapart, Professor , Dr.Kangsadan Boonprab, Associate Professor ,  J. Praiboon, O. Bhumibhamon, J. Phothikasikorn, Yoshihiko Akakabe, Kenji Matsui, Tadahiko Kajiwara, การประชุมวิชาการ: |
 หัวเรื่อง:การหมักโกโก้ I : การจำแนกและศึกษาเมตาบอไลต์ของจุลินทรีย์จากการหมักโก้โก้ธรรมชาติ ผู้เขียน:นางอรพิน ภูมิภมร, รองศาสตราจารย์ , antana Jinda สื่อสิ่งพิมพ์:pdf Abstract840 isolated microorganisms during cocoa fermentation were studied. Sixhundred and thirty isolated bacteria were found in 4 families as Lactobacillaceae, Micro-coccaceae, Bacillaceae, Aectobactereae and in 5 genera of Lactobacillus sp., Micrococcus sp.Bacillus sp., Acetobacter sp. and Gluconobacter sp. Other 210 isolated yeasts were in family of Sporobolomycetaceae, Saccharomycetaceae and in genus of Candida sp., Kluyveromyces sp., and Saccharomyces sp. Two hundred and ten isolates each of yeast, bacteria and acetic acid bacteria were investigated for their efficiencies in alcohol (yeast), enzyme pectinase (yeast and bacteria) and acetic acid (acitic acid bacteria) formation. Yeasts produced ethyl alcohol in the amount of 0-10 mg/100 ml. Pectinase-producing yeasts and bacteria produced enzyme pectinase showed pectin degradation clear zone 0-9 mm. and 0-6 mm., respectively. Acetic acid bacteria produced acetic 0-0.4 gm/100 ml. According to the results of metabolites produced, eleven isolated microorganisms were chosen for further cocoa fermentation studied. The selected alcohol-producing yeasts were identified as Saccharomyces cerevisiae (KU-Y77), Candida utilis (KU-Y79), Candida krusei (KU-Y125); pectinase-producing yeasts and bacteria were Saccharomyces chevalier (KU-Y150) and Bacillus sp. (KU-B190), respectively; acetic bacteria were Acetobacter ranceus (KU-A40), Gluconobacter oxydans (KU-A45), Acetobacter acetic (KU-A72; KU-A187), Acetobacter pasteuriences (KU-A101) and Acetobacter persoxydans (KU-A132) |
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หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Lipase-Producing Microorganisms for Use in Contaminated Fat and Oil Kitchen Wastewater Treatment) ผู้เขียน:นางอรพิน ภูมิภมร, รองศาสตราจารย์, ดร.เกรียงไกร พัทยากร, ผู้ช่วยศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractBiodegradation of fats and oils in wastewater has a potential role in pollution control. Then, selection of efficient microorganisms for degrading fats and oils in laboratory level has been investigated by using single culture of Acinetobacter sp. (KUL8), Bacillus sp. (KUL39) and Pseudomonas sp. (KLB1) and mixed cultures of Acinetobacter sp. (KUL8) and Pseudomonas sp. (KLB1). The result showed that single culture of Pseudomonas sp. (KLB1) and mixed cultures of Acinetobacter sp. (KUL8), Bacillus sp. (KUL39) and Pseudomonas sp. (KLB1) could be reduced fat and oil content by 55.91% and 60.42%, respectively. These cultures were shown highest efficiency activities. Thus, these cultures were selected to study speed of shaking and inoculum size in wastewater treatment, which it was found that the efficiency of fat and COD degradation were highest reduced at 250 rpm of speed of shaking. Inoculum size at 1-2.5 % and 5 % of single culture and mixed cultures could remove fat and oil by 61.78-65.19 % and 60.42 %, respectively. And initial COD concentration at 4,000 mg/l has the highest efficiency 83.46% and 95.81% in fat and COD degradation, respectively. The last experiment, Pseudomonas sp. (KLB1) was used in occasionally influent and effluent contaminated fat and oil kitchen wastewater treatment in reactor. The results showed that the highest efficiency of aerobe conditions were 50.02% and 0.76 U/ml, respectively in fat degradation and lipase activity after adding 8% of single culture at 22 days. After that lipase activity was reduced because influent and effluent of wastewater in reactor depended on activity of kitchen. Since dilution rates (D) of wastewater in reactor were higher than specific growth rate (?), so microorganisms were washed out. Thus, the immobilization of microorganism cells on plastic balls has been used. The results showed that cells immobilization could increase efficiency of wastewater treatment in reactor. |
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หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Production of Glucoamylases by A Rotting Cassava Tuber Fungus on Solid Culture) ผู้เขียน:นางอรพิน ภูมิภมร, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractAspergillus niger H-9 is a fungal strain isolated from rotting cassava tuber in Thailand. In the present study, the strain was found to produce better glucoamylase on solid culture for 3-4 days. (ricebran and soybean meal in the ratio of 9:1). The moisture content for cultivation was found to be 3:1 of solid-liquid ratio. The suitable initial pH and temperature were 5-6 and 30 C. The highest enzyme yield was found to be 1,080 units. Optinum pH of the enzyme for starch hydrolysis was at pH 5.5. Such enzyme shows pH stability between 4-8, temperature activity at 60-70C and temperature stability between 30-70C. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : The Optimum use of Salinity, Nitrate and Pond Depth for b-Carotene Production of Dunaliella salina) ผู้เขียน:นางอรพิน ภูมิภมร, รองศาสตราจารย์, นายอุดม สิทธิภู่ประเสริฐ, อาจารย์, Naiyana Boontaveeyuwat, ดร.จันทนา ไพรบูรณ์, ผู้ช่วยศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractDunaliella salina, halotorelant green algae was collected from the East Coast of Thailand. It has a massive accumulation of b-carotene when grown under defined growth conditions such as high light intensity, high salt concentration and nitrate deficiency. The present study investigated the optimization of salinity, nitrate and pond depth for cell growth and b-carotene production of the alga in question. Cultivation was done in three stages. These were indoor cell growth cultivation, outdoor cell growth cultivation and outdoor b-carotene production. The optimum salinity for cell growth of indoor and outdoor cultivation (5 l) was 9% NaCl, which has specific growth rate (m) of 0.579 (d-1) and 0.981 (d-1). The optimum salinity for outdoors b-carotene production (5 l) when use 40% inoculum (2.16 ? 106 cell ml-1) was 12% NaCl. This produced b-carotene content of 51.73 mg ml-1. In addition, the concentrations of medium used were 100%, 75% and 50% to decrease nitrate concentration in the starter ponds for increasing b-carotene production in the next step. The results showed that 50% medium gave minimum nitrate concentration of 4.5 mg l-1. For the effect of pond depth, the light expose of the alga were used 9, 11, 13 and 18 cm the ratios of carotenoid to chlorophyll were 7.48, 6.25, 5.54 and 3.35, respectively. Therefore the suitable pond depth for b-carotene production from D. salina was 9 to 11 cm. |
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หัวเรื่อง:ผลิตภัณฑ์จากแป้ง สตาร์ชสกัด และโปรตีนสกัดของเมล็ดถั่วเขียว ผู้เขียน:ดร.อรอนงค์ นัยวิกุล, ศาสตราจารย์ , นางจิตธนา แจ่มเมฆ, ผู้ช่วยศาสตราจารย์, นางอรพิน ภูมิภมร, รองศาสตราจารย์, Somjit Permsub สื่อสิ่งพิมพ์:pdf AbstractUsing the mungbean flour, starch and protein of mungbean were isolated to make various products. The results showed that the noodle which was made from mungbean flour mixed with isolated starch of mungbean VC. 2742 variety had firm thread. The color of mungbean noodle was yellow which was different from the white color of normal noodle. The Khaw – Kreab made from isolated starch of mungbean VC. 2755 variety had white color, thin and crisp. Mungbean milk was made from isolated protein of mungbean VC. 2745 variety, contained 1% CMC, showed the characteristics of milk to be thicken and yellowish color. The taste of beany – flavor of mungbean milk was not pronounced. |
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หัวเรื่อง:การหมักโกโก้ III: การปรับปรุงการหมักโกโก้โดยเสริมการหมักด้วยกล้าเชื้อผสมในระดับห้องปฏิบัติการและเกษตรกร ผู้เขียน:นางอรพิน ภูมิภมร, รองศาสตราจารย์, Jantana Jinda, บุญให้ แหลมเพชร, Piyanut Naka สื่อสิ่งพิมพ์:pdf AbstractIn studied improvement of cocoa fermentation by inoculated with selected culture in laboratoryscale the microorganisms used were 3 strains of alcohol-producing yeasts, pectinase-producing yeasts and bacteria, and 6 strains of acetic acid bacteria. After cocoa fermentation in 5 kg batches of each strains, the strains which showed better results in fully fermented (4/4F) cocoa by cut-test method were Saccharomyces cerevisiae (KU-Y77), Saccharomyces chevalieri (KU-Y150) and Acetobacter aceti (KU-A72). The optimum ratio of these strains in 20 kg cocoa batches were 1.5:1.0:1.0 (% weight) which given fully fermented cocoa in 81.7-83.5% where as 43.7-61.0% were found in natural fermentation batches. Four farm-trials cocoa fermentation experiment with inoculated mix-culture were carried on 4 and 6 days. The results showed fully fermented cocoa in 79-84% which was indicated as cocoa FAO grade 1 standard. In the same time, the cocoa fermentation period was able to reduce from 6 days to 4 days. Anyhow, the utilization of inoculum in small scale (20-40 kg) showed promising. However, the quality of mix-culture fermented cocoa should be further investigated by sensory evaluation method. |
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หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Biotreatment of High Fat and Oil Wastewater by Lipase Producing Microorganisms) ผู้เขียน:นางอรพิน ภูมิภมร, รองศาสตราจารย์, อัจฉรา คอประเสริฐศักดิ์, Suptawee Funthong สื่อสิ่งพิมพ์:pdf AbstractStudies on biotreatments of high fat and oil wastewater by selected lipase producing bacteria were carried out in two experiments with two groups of bacteria, single culture and mixed culture. In the first experiment, wastewater of bakery industry was treated with 4 bacteria isolates. Results showed that grease in the form of fat and oil and COD decreased remarkably with treatments. With single culture, the removal of fat and oil and COD were 73%-88% and 81%-99% during 7 days treatment. Isolates KUL8 and KUL39 showed better activities. However, better treatments were found in experiment treated with single culture than using mixed culture. In the second experiment, wastewater of palm oil and bakery industries were treated with 6 isolates within 48 hours. All 6 isolates showed better degradation in palm oil wastes than bakery wastes. However, KUL8 and KUL39 still showed better degradation activities. Both isolates can removed fat and oil by 87.7% and 80.6% in palm oil wastewater and 70% and 64% in bakery wastes respectively. The decreasing of COD was found to be 90%-96%. When mixed culture of KUL8, KUL39 and KLB1 were applied for both kinds of wastewater. It was found that better results were observed in palm oil treated with single culture KUL8, KUL39, and KUB1 while mixed culture KUL8 mixed with KLB1 was suitable for use in bakery waste. The three isolates were identified as Acinetobacter sp. (KUL8), Bacillus sp. (KUL39), and Pseudomonas sp. (KLB1). They were all mesophilic strains. Besides, it was found that all three strains also produced amylase and protease which stimulate better waste treatments. |
หัวเรื่อง:การหมักโกโก้ : ศึกษาการเปล่ยนแปลงทางจุลนทรีย์ ฟิสิกส์ และเคมีในระหว่างการหมักโกโก้ ผู้เขียน:นางอรพิน ภูมิภมร, รองศาสตราจารย์, Piyanuch Naka, Umpol Julsawad สื่อสิ่งพิมพ์:pdf AbstractStudies on environmental change during cocoa fermentation were carried out in baskets and wooden boxes, storing cocoa pods for 4 and 7 days after harvesting. During fermentation materials were sampled and ana?lysed at intervals. Beans fermented in boxes shiftly better cut test value than those kept in baskets. Fully fermented (F) bean of 54-6796 , and 28-4596 of 3/4 fermented beans were found in boxes, 50-5696 of fully fermented beans, 43?49% of 3/4 fermented beans in baskets. All fermented batches showed slaty less than 3%. The proximate analysis showed that fat content in unfermented and fermented beans were similar, at 5596, Ash and fiber in fermented beans was slightly less than in unfermented beans. The protein content decreased 1?296 by the end of the fermentation. The temperature during fermentation reached 45?C, after storage of cocoa pods for 4 days and 7 days, in 48 and 24 hours respectively. Mesophilic and thermophilic yeast and bacteria were also found. The pH of the fermented cocoa both in baskets and boxes were at 4.7-6.7 |
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