Search Result of "Mutsumi Takagi"

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ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : The Influence of Serum Concentration on tPA Production of CHO Cell)

ผู้เขียน:ImgTeerapatr Srinorakutara, ImgJin-Ho Jang, ImgMutsumi Takagi, ImgToshiomi Yoshida



The effect of initial serum concentration on cell growth, and the production of tissue plasminogen activator (tPA) is investigated across batch fermentation of a Chinese hamster ovary (CHO) growing in newborn calf serum (NCS) medium. It was found that initial serum concentration influenced on CHO cell growth rate and cell yield. At high-serum content, the uptake rate of glucose decreased although the specific growth rate was higher, indicating a serum component becoming growth limiting. An average glucose uptake rate in 10% (v/v) NCS medium was 13.84 mg/106 cells/h as in 1% (v/v) NCS medium was 18.17 mg/106 cells/h. It was also found that the tPA or the maximum tPA productions increased with initial serum concentration. At 7.12% NCS, the maximum tPA production gave the highest value (5.55 mg/l). The effect of initial serum concentration on the main product was similar to that of major by-product (e.g. lactate and ammonia).

Article Info
Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 034, Issue 1, Jan 00 - Mar 00, Page 79 - 84 |  PDF |  Page