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ผลงานตีพิมพ์ในวารสารวิชาการการสร้างเครือข่ายความร่วมมือพหุภาคีเพื่อการพัฒนาคุณภาพการศึกษาของโรงเรียนขนาดเล็กจังหวัดนครปฐม (2015)ผู้แต่ง: Dr.sumit suwan, Associate Professor , Mr.athikiat thongperm, Associate Professor , Dr.surin Xoomsai Na Ayudhya, Assistant Professor , Dr.Tussatrin Wannagatesiri, Associate Professor , Dr.Nantarat Kruea - In, Assistant Professor , Dr.sukhon maneerat, Assistant Professor , Dr.Kulthida Nugultham, Assistant Professor , Dr.Witat Fakcharoenphol, Assistant Professor , วารสาร: 
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ผลงานตีพิมพ์ในวารสารวิชาการEvaluating agro-industrial by-products as dietary roughage source on growth performance of fattening steersผู้แต่ง: Maneerat, W., Dr.Somkiert Prasanpanich, Associate Professor , Dr.Sornthep Tumwasorn, Professor , Laudadio, V., Tufarelli, V., วารสาร: 
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ผลงานตีพิมพ์ในวารสารวิชาการEffect of feeding total mixed fiber on feed intake and milk production in mid-lactating dairy cowsผู้แต่ง:Maneerat, W., Dr.Somkiert Prasanpanich, Associate Professor, Dr.Phongthorn Kongmun, Assistant Professor , Sinsmut, W., Dr.Sornthep Tumwasorn, Professor, วารสาร: |
ผลงานตีพิมพ์ในวารสารวิชาการDynamics of a low-density tiger population in Southeast Asia in the context of improved law enforcementผู้แต่ง:Duangchantrasiri, S., Umponjan, M., Simcharoen, S., Dr.Anak Pattanavibool, Lecturer , Chaiwattana, S., Maneerat, S., Kumar, N.S., Jathanna, D., Srivathsa, A., Karanth, K.U., วารสาร: |
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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Construction of Single-Chain Variable Fragment (scFv) Specific to Cucumber Mosaic Virus by Phage Display Technology) ผู้เขียน:Maneerat Koohapitagtam, Suang Rungpragayphan, ดร.รัชนี ฮงประยูร, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf Abstract Cucumber mosaic virus (CMV) causes serious problems in economically important crops, especially members of the Solanaceae and Cucurbitaceae families. Serological detection of this virus by specific antibodies is required as a control measure as well as for quarantine investigation to ensure any components used for agricultural propagation, especially commercial seeds, are disease free. However, the selection of recombinant antibodies by phage display nowadays presents a real challenge to provide the antibodies that are urgently needed. In this research, an anti-CMV single-chain variable fragment (scFv) was constructed using a phage display system. Both heavy (VH) and kappa light chain variable (Vk) genes were amplified by RT-PCR from the hybridoma cell line CM2, secreting a monoclonal antibody (MAb) specific to both serogroup I and II of CMV. The VH and Vk amplified products, approximately 400 bp in length, were joined by a PCR overlapping extension method to generate the scFv gene. A recombinant phagemid pCANTAB5E harboring the scFv gene was constructed and transformed into Escherichia coli TG1. The bacterial transformants were rescued by helper phage M13 to produce phage-displayd scFv and the screening for CMV-specific scFv was carried out by ELISA. Three positive, recombinant clones (2C1, 6A1 and 1D4) which gave high signal-to-noise in ELISA were utilized in order to produce soluble antibodies. Western blotting and DNA sequencing were performed to characterize the scFv products. The result showed that all clones were identical and able to bind CMV of both subgroups. DNA comparisons showed that all the VH belonged to the J558.32 subgroup and JH2, while Vk belonged to Vk genes and JK2. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Ex-situ propagation of Pogostemon helferi (Hook. f.) Press using tissue culture and a hydroponics system) ผู้เขียน:Maneerat Wangwibulkit, ดร.สรัญญา วัชโรทัย, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractPogostemon helferi (Hook. f.) Press, locally known as “dao-noi” is a rare Thai indigenous aquatic plant that is popular for use in aquaria and water gardens. To address its scarcity and to make the plant more readily available, two experiments were conducted to find the optimum conditions for ex-situ propagation. The first experiment aimed to determine the concentration of growth regulators for its micropropagation. Sterile explants were cultured using a combination of 3-indoleacetic acid (IAA) and 6- benzylaminopurine (BA) supplements in Murashige and Skoog media (MS). MS media containing IAA 0.1 mg/L and BA 3 mg/L induced the highest percentage of callus formation (93.33%). In addition, MS media with IAA added at 0.3 mg/L significantly (p < 0.05) increased the number of new shoots appearing and their length after 8 wk. The second experiment aimed to determine the optimum electrical conductivity of the nutrient solution and the humidity level for P. helferi growth within a hydroponics system. The results showed that an electrical conductivity of 1.6 mS/cm and 80% humidity were optimal for P. helferi growth and production to a marketable size. These methods should enable the production of P. helferi appropriate to support market demand and thus can reduce the current practice of harvesting wild plants in their natural habitat. |
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