 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Effect of Acetic Acid on Growth and Ethanol Fermentation of Xylose Fermenting Yeast and Saccharomyces cerevisiae) ผู้เขียน: ดร.สาวิตรี ลิ่มทอง, ศาสตราจารย์ ,  ธวัชชัย สุ่มประดิษฐ, ดร.วิเชียร กิจปรีชาวนิช, รองศาสตราจารย์ , Manee Tuntirungkij, Tatsuji Seki, Toshiomi Yoshida สื่อสิ่งพิมพ์:pdf AbstractGrowth of some xylose fermenting yeasts, Candida shehatae, Pichia stipitis CBS5773, fusant F101 and fusant F198, was completely inhibited in xylose medium added with 0.5% v/v acetic acid which caused the reduction of pH to 4.1. Only one xylose fermenting strain, Pachysolen tannophilus NRRL-Y2460, showed relatively low growth and ethanol fermentation. However, in the medium added with 1.0% v/v acetic acid (pH 3.7) all of these strains were completely inhibited. When the medium was adjusted by hydrochloric acid to pH 4.1 and 3.7, all xylose fermenting strains showed almost the same growth as in the medium without pH adjustment (pH 6.2). In glucose medium added with 0.5% v/v acetic acid, various strains of Saccharomyces cerevisiae, M30, Sc90, N1, G/3, G/5, G/2, TJ3 and SH1089, grew with lower specific growth rate and provided lower maximal cell concentration rate than in medium without adding acetic acid (pH 6.2). All strains, except N1, produced slightly higher maximal ethanol concentration. However, all of them yielded lower ethanol production rate. Among S. cerevisiae, strain B120 was more sensitive to acetic acid than the others since its growth was completely inhibited by 0.5% v/v acetic acid. In glucose medium, 0.5% v/v acetic acid did the same role as in xylose medium to xylose fermenting strains. Hence, the xylose fermenting yeasts revealed higher sensitivity to acetic acid than S. cerevisiae. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Characterization of Microsatellite Markers for the Siamese Crocodile and Amplification in the Closely Related Genus Crocodylus) ผู้เขียน:ดร.วิน เชยชมศรี, รองศาสตราจารย์ , ดร.สุดาวรรณ เชยชมศรี , Manee Tuntirungkij, ประทักษ์ ตาบทิพย์วรรณ, นางนภาวรรณ นพรัตนราภรณ์, รองศาสตราจารย์, ดร.วรวิทย์ สิริพลวัฒน์, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractTwenty microsatellite markers from Crocodylus siamensis were characterized in 40 crocodiles: 29 C. siamensis, 4 C. porosus, 2 hybrids (C. siamensis + C. porosus) and 5 Caiman crocodilus crocodilus. Fourteen microsatellite markers that showed specific alleles were considered as specific markers for use in identifying the species of crocodile. These markers were: CS-25 for C. siamenis; CS-4, CS-26 and CS-30 for C. porosus; CS-2, CS-7, CS-10, CS-12, CS-14, CS-17, CS-22, CS-24 and CS-35 for C. c. crocodilus; and CS-20 for all species. Twelve microsatellite markers that had polymorphic alleles in the same species were considered as intra-specific markers. These markers were: CS-4, CS-5, CS-10, CS- 12, CS-17, CS-18, CS-21, CS-24, CS-26, CS-30, CS-32 and CS-35. Seven microsatellite markers showed an absence of alleles in C. c. crocodilus and were considered as genus-specific markers. These markers were: CS-4, CS-5, CS-18, CS-21, CS-26, CS-30, and CS-32. The microsatellite markers mentioned above could be used for species testing and could also be evaluated to help determine parentage in situations where the maternity, paternity, or both were unknown. It was therefore recommended that more microsatellite markers be used in such situations. The use of the microsatellite markers involved in this study would broaden the scope of a breeding program, allowing progeny from adults maintained in large breeding lagoons to be tested for selection as future breeding animals. |