 ผลงานตีพิมพ์ในวารสารวิชาการInfluences of food matrices on cytotoxicity of asiatic acid in mammalian cell modelsผู้แต่ง: Jirasripongpun, K., Jirakanjanakit, N., Sakulsom, P., Wongekalak, L.-O.,  Dr.Parichat Hongsprabhas, Associate Professor , วารสาร:  
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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Risk Assessment of Triclosan Using Animal Cell Lines) ผู้เขียน:Kalyanee Jirasripongpun, Thanate Wongarethornkul, Sunisa Mulliganavin สื่อสิ่งพิมพ์:pdf AbstractTriclosan is an antimicrobial ingredient used in many health-care products and household items including plastics, textiles and food packaging materials. However, the safety of triclosan has been questioned recently. In this study, cytotoxicity of triclosan to BHK-21, Vero and KB cells were performed by incubating these cells in culture medium containing various concentrations of triclosan for 3 days. Vero and KB cells were more sensitive and suitable as models to evaluate triclosan toxicity. The 50% inhibition concentration (IC50) of triclosan to Vero and KB were at 0.036 and 0.034 mM, respectively, while that of BHK-21 was at higher concentration (0.26 mM). Analyzing for triclosan content in popular use of personal health-care products revealed the maximum triclosan content at 0.27 % (w/w) or 0.023 mM in liquid facial soap. Genotoxicity analysis based on comet assay demonstrated the DNA breakage by triclosan. Its genotoxic potency was significantly increased as the triclosan concentration and the exposure time increased. Exclusion of cell death or cytotoxicity, the genotoxic effect of triclosan in this study could be observed noticeably at the IC20-30 of triclosan after 5 days treatment. Therefore, animal cell lines could be used for primary evaluation on the risk assessment of triclosan. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Carotenoids Production by Xanthophyllomyces dendrorhous Mutant Grown on Molasses) ผู้เขียน:Kalyanee Jirasripongpun, Wachilaporn Pewlong, Wilawon Natsathmonthra, Suthirut Suthiyaporn สื่อสิ่งพิมพ์:pdf AbstractAstaxanthin, the main carotenoid pigment in dietary red yeast of Xanthophyllomyces dendrorhous, has a huge market potential as fleshy colourant and supplementary food to animal production and health. Cost of carotenoid production would be reduced by using a carotenoid-hyperproducing mutant and a cheap medium of molasses. Therefore, either mutant yeast [mutant GM807 (derived from gamma irradiation) or mutant n485 (from neutron irradiation)] was selected based on growth and carotenoid production in 5% (v/v) molasses containing 30 g/l urea and 4.5 g/l sodium phosphate under shaking condition for 10 days. The mutant GM807 was superior to mutant n485 in 1.5 fold higher of carotenoid content. Medium formula development for mutant GM807 revealed that beef extract and potassium nitrate enhanced higher carotenoid content than urea did. Concentration of molasses had an effect only on yeast growth but not on carotenoid formation. Total carotenoid content of 450 ?g/g of yeast was obtained at the optimum pH of 4. Therefore, molasses (2% v/v) containing 4 g/l potassium nitrate, at pH 4, could possibly be an alternative culture medium for the mutant GM 807 to produce carotenoids. |
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