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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Nucleotide Polymorphism of Starch Synthesis Genes in Thai Rice) ผู้เขียน: Siriphorn Jangsutthivorawat, Hugo Volkaert สื่อสิ่งพิมพ์:pdf AbstractStarch composition and cooking quality are important rice-grain characteristics of interest to breeders. One hundred and ninety-two accessions of Oryza, mostly from Thailand, were assayed using PCR-SSCP and alleles were identified at seven starch-synthesis gene loci (GBSSI, SSSIIIa, SSSIIIb, SSSIVa, SSSIVb, RBE1 and RBE3). The nucleotide sequences were obtained corresponding to each of the SSCP patterns observed and the sequences were submitted to GenBank (accession numbers EF990806 – EF990893). The frequency of nucleotide polymorphisms at these loci was about 1 SNP per 29 bp. A total of 136 nucleotide polymorphisms (SNPs), 16 insertions/deletions (indels) and 4 SSRs were identified. The SSRs were found at the GBSSI (CT, AATT), SSSIVb (GGGTT) and RBE1 (CT) loci. The nucleotide diversity, as measured by ?, ranged from 0.00086 (RBE3) to 0.02747 (SSSIVa) and nucleotide polymorphism, as measured by q, ranged from 0.00154 (RBE3) to 0.02337 (SSSIVa). The Oryza accessions from Thailand in this study displayed a high nucleotide diversity compared with previous estimates of Oryza species based on estimates of multiple loci. |
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หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Chloroplast Diversity and Phylogeny in Wild and Cultivated Rice (Oryza spp.)) ผู้เขียน:ศิริพร ช่วยแจ้ง, Hugo Volkaert สื่อสิ่งพิมพ์:pdf AbstractGenetic diversity was studied in the chloroplast genome of wild and cultivated rice, especially in species belonging to the A genome group, to clarify the phylogenetic relationships in Oryza. Specific DNA fragments were PCR-amplified from 140 accessions of cultivated and wild rice and investigated by restriction analysis and SSCP (PCR-RF-SSCP or PRS). After the SSCP patterns were scored, amplified fragments from representative accessions of each observed SSCP pattern were sequenced. Haplotypes of cultivated rice and closely related wild relatives (A genome) were more similar than those of distantly related species (B, C/CD, E and G genomes). The polymorphisms were due to insertion/deletion (indels) and nucleotide substitutions. Substitutions and indels at ORF100, and rpoA locus could separate the Asian cultivated rice indica type from japonica type. Additional substitutions and indels at all four cpDNA loci could separate the rice species with the AA genome from those belonging to other genomes (but these mutations were not congruent with indica/japonica division). Within the AA genome four groups could be separated, Asian wild rice with Asian cultivated rice, O. glumaepatula group, African wild rice with African cultivated rice (O. glaberrima) and O. meridionalis group. |
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หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Genetic Diversity of Andrographis paniculata Wall. ex Nees as Revealed by Morphological Characters and Molecular Markers) ผู้เขียน:ตถาพร ไม้สน, Hugo Volkaert,  ดร.อุณารุจ บุญประกอบ, รองศาสตราจารย์ , ดร.ยิ่งยง ไพสุขศานติวัฒนา, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractIntraspecific variation of twenty-five accessions of Andrographis paniculata Wall. ex Nees, collected from different geographical areas in Thailand and one from Laos, was determined by morphological characters and molecular analysis using RAPD and SSCP techniques. Morphological characters and total lactone content variation were measured at 50% flowering time among the accessions grown under uniform environmental condition. UPGMA cluster analysis of all accessions resulted in 4 major groups based on 18 morphological characters while RAPD analysis distinguished 5 groups at 0.82 of similarity coefficient, using 247 bands generated from 14 primer amplifications separated on polyacrylamide gel. Morphological characters and RAPD were incongruent and were not correlated to geographical area of collection and yield of active compound. SSCP analysis showed little polymorphism of specific amplified products. Markers for the CPS1-2, IDH1, IDH2 and IPI genes were monomorphic and only 2 alleles were detected for CAT and CPS1-1 genes. |
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หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Development of Catalase Gene Nuclear DNA-Based Marker for Population Genetic Analysis in Thai Teak (Tectona grandis L.f.)) ผู้เขียน:Jongkon Cheua-ngam, Hugo Volkaert สื่อสิ่งพิมพ์:pdf AbstractThe teak Catalase gene was amplified and cloned using consensus PCR primers designed based on sequences from other plant species. The obtained teak Cat DNA sequences were used to develop specific primers. The specific primer set could successfully amplify a specific DNA fragment from teak in all populations studied. Using PCR-SSCP, 5 different alleles were detected. The tested nuclear gene primer set had considerable potential as a DNA marker for population analysis in teak. This approach could be used to specifically amplify fragments in other plant species and applied to study evolution, population genetics, outcrossing rate and mating patterns. |
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