Search Result of "Embryos"

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งานวิจัย

ผลของสารสกัดพริกไทยดำต่อเอมบริโอปลาม้าลาย (2013)

หัวหน้าโครงการ:Imgดร.กันทิมาณี พันธุ์วิเชียร ประเดิมวงศ์, ผู้ช่วยศาสตราจารย์

แหล่งทุน:ภาควิชาสัตววิทยา คณะวิทยาศาสตร์ มหาวิทยาลัยเกษตรศาสตร์

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ผลงานตีพิมพ์ในวารสารวิชาการ

Induction of somatic embryos of Coffea arabica cv. Catimor CIFC 7963 using spirulina extract

ผู้แต่ง:Imgน.ส. สุนิสา ฤทธิสังข์, ImgDr.Noppamart Lokkamlue, Assistant Professor,

วารสาร:

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ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Studies on the formation adDevelopmen of Tangerine’s Embryos)

ผู้เขียน:ImgVatana Satiansavat, ImgRavee Pachdikulsumpun

สื่อสิ่งพิมพ์:pdf

Abstract

Tangerine flowers arise from terminal as well as ateralbuds. The flowers are of perfect and solitary types. The inflorescences arrange cymosely from the terminal branchwith 4-6 petals, 5 sepals, 16-24 stamens and 10-14 carpels. Each carelconsists of 4-5 ovule arranged in the way of axil placentation. The anther consists of two lobes, with two locules in each lobe. The moicrospore tetrad has tetrahedral arrangement and appears as binucleated pollen. The megaspore tetrad has linear arrangement. The mbryo sac develops normally or as the polygonum type in the ovule. The receptive time for stigma is 2-3 days before blooming, and double fertilization occurs 4 days after pollination. The zygote divides 45 days after fertilization and develops into gametic embryo at about the same tim s nucellar embryo development which takn place near the micropylar end. The endosperm develops as the nuclear type. The largest embryo may be either nucellar or gametic embryos. If the nucellar embryo quickly develops and becomes large the gametic embryo may degenerate. The reasons of polymbryo formation in tangerine are:- a) Cleavage of zygote or fission of gametic embryo. b) There are two embryo sac in one ovule and both embryo sacs develop into gametic embryo. c) Nucellus cell divides into nucellar mbryo. d) Budding of nuclear embryo.

Article Info
Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 007, Issue 2, Dec 73 - Dec 73, Page 126 - 126 |  PDF |  Page 

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ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Production of Cloned Asian Elephant Embryos Using an Interspecies Somatic Cell Nuclear Transfer (iSCNT) Technique)

ผู้เขียน:ImgAnucha Sathanawongs, ImgYada Jarujinda, Imgนายสุวิชัย โรจนเสถียร, ผู้ช่วยศาสตราจารย์, ImgApichart Oranratnachai

สื่อสิ่งพิมพ์:pdf

Abstract

This study was carried out to investigate the feasibility of using interspecies somatic cell nuclear transfer (iSCNT) techniques in Asian elephants (Elephas maximus), using elephant fibroblasts as donor cells and rabbit oocytes as the recipient cytoplasts. Elephant fibroblasts were collected post-mortem from the ear skin of a female Asian elephant and cultured in vitro. Monolayer fibroblasts were trypsinized and used for SCNT. The comparative study showed that the blastocyst rates from iSCNT were significantly lower (P<0.05) than for rabbit SCNT and rabbit parthenogenesis (19.4, 56.7, and 70.3%, respectively). The study tried to improve the efficiency of iSCNT by using phytohemagglutinin-P (PHA-P) to increase the fusion rate and nocodazole to synchronize donor cells in the G1 stage of the cell cycle. The fusion rate of elephant-rabbit couples treated with PHA-P was significantly increased (47.5%) compared with the non-treated group (26.2%; P<0.05). The cloned embryos could develop into blastocysts (27.8%), which was significantly (P<0.05) higher than for the non-treated cells group (14.4%). By using this protocol, Asian elephant blastocysts can be produced by iSCNT of fibroblast cells into rabbit cytoplasts with the use of nocodazole and PHA-P. The blastocyst rate of cloned elephant embryos using rabbit cytoplasts was more than 25% and may have potential in further studies on elephant preservation.

Article Info
Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 044, Issue 4, Jul 10 - Aug 10, Page 610 - 620 |  PDF |  Page 

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