Search Result of "Busaba Panyarachun"

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ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Effects of Pharmaceutical Mestranol on Estrogen Receptor ? mRNA Expression Levels and Morphometry in the Anal Fins of Adult Thai Ricefi sh (Oryzias minutillus))

ผู้เขียน:ImgArin Ngamniyom, Imgกัญจน์ ศิลป์ประสิทธิ์, ImgBusaba Panyarachun



This study aimed to examine the estrogen receptor (ER) ? mRNA expression levels and morphometry of the anal fi n of adult Thai ricefi sh (Oryzias minutillus) after treatment with pharmaceutical mestranol. Time- and concentration-related courses of mestranol on ER? expression were investigated in anal fi ns by semi-quantitative reverse transcription polymerase chain reaction. In the time course experiments, ER? expression levels in male anal fi ns were increased at 30 d after treatment with 10 ?g.mL-1 of mestranol. ER? levels in female anal fi ns were highly expressed at 21, 30 and 60 d after mestranol treatment. In the concentration-response experiment, ER? levels were increased in males after treatment with 10 ?g.mL-1 of mestranol for 21 d and in females treated with 0.1, 1, or 10 ?g.mL-1 mestranol for 21 d. Morphometrical analysis showed that the proportion of anal fi n length to standard length was low in males after 10 ?g.mL-1 mestranol treatments for 30 and 60 d. In contrast, there was no morphometrical difference in females after exposure to mestranol in any of the treatments analyzed. The results suggested that mestranol regulates the sex-dependent morphology of anal fi n in development at least in part by mediating ER? expression and may lead to the disruption of endocrine activities in fi sh.

Article Info
Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 046, Issue 1, Jan 12 - Feb 12, Page 80 - 90 |  PDF |  Page