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ผลงานตีพิมพ์ในวารสารวิชาการHaplotype variation and phylogeography of Rhizoctonia solani AG1-IA strains based on rDNA5.8S-ITS and -actin gene sequence analysesผู้แต่ง:Wei, Y, Bao, JD, Cao, HJ, Zhai, J, Dr.Chatchawan Jantasuriyarat, Associate Professor, Zuo, SM, Pan, XB, Wang, H, Zhou, B, วารสาร: |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : An Analysis of cis-Acting Regulatory Elements Related to Light Response in the 5’ Flanking Region of the Ascocenda and Dendrobium Actin Genes) ผู้เขียน:พัฒนา ศรีฟ้า ฮุนเนอร์ สื่อสิ่งพิมพ์:pdf AbstractIn a previous study, the rice actin promoter was considered as a constitutive promoter, which could drive reporter gene expression in orchid flowers. Therefore, in this study, the 5’ flanking regions of the actin genes isolated from Ascocenda Princess Mikasa ‘Blue’ and Dendrobium Jacquelyn Thomas ‘UH44-50’ orchids were validated for predicted cis-acting regulatory elements (CAREs) using the PlantCARE program and the PLACE database. A high frequency of predicted CAREs, which were distinct in numbers and locations, particularly the CAREs that respond to light, was found within the upstream flanking regions of the actin gene in orchids, rice and banana. Both orchid upstream sequences contained some predicted CAREs that are predominantly involved in light regulation. When orchid flowers were exposed to different light periods, the expression of vegetative actin in Ascocenda flower tissues (column, lip and petal) was completely up-regulated, as determined by qualitative real-time polymerase chain reaction. In addition, all of the 5’ flanking regions of orchid, rice and banana actin genes contained large numbers of the transcriptional control sequences, TATA boxes, which were composed of similar sequences to the conserved TATA box of vegetative actin. The further investigation of the 5’ flanking region of orchid actins for their activities will be essential in the development of these promoter regions for biotechnological use. |
ผลงานตีพิมพ์ในวารสารวิชาการDetection of DNA using gold nanoparticle-coated silica nanoparticlesผู้แต่ง:Abdelrazig, A.O., Rijiravanich, P., Dr.Sawita Suwannarat, Assistant Professor, Surareungchai, W., Somasundrum, M., วารสาร: |
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หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Evaluation of Constitutive Promoters for Gene Expression in Dendrobium Protocorms and Flowers) ผู้เขียน:Chidchanok Suwannaketchanatit, พรสุข ชัยสุข, Jitraparn Piluek, ดร.สุรินทร์ ปิยะโชคณากุล, รองศาสตราจารย์, พัฒนา ศรีฟ้า ฮุนเนอร์ สื่อสิ่งพิมพ์:pdf AbstractActively dividing protocorms of Dendrobium Jaquelyn Thomas orchid were produced on VW medium and transferred to 1/2 MS medium before subjecting to particle bombardment to evaluate the promoter efficiency. Three different constitutive promoters, rice Act-1, CaMV35S, and maize Ubi-1, were determined for the efficiency to control spatial transgene expression in protocorms and sepal tissues of Dendrobium orchid. Each promoter was fused to b-glucuronidase (uid A) gene and nos terminator in the plant expression vectors including pActin1-D, pAHC27, and p2K7. The plasmid vectors were individually bombarded into protocorms and fresh sepals of Dendrobium. Transient gus gene expression was assayed after 3 days of bombardment. The results demonstrated that all tested promoters could drive gus gene expression in the orchid protocorms at different levels. Ubi-1 promoter conferred the most efficient promoter in protocorms giving the highest level of gus gene expression, whereas GUS activity was distinctly observed under the control of Act-1 and CaMV35S in the Dendrobium sepal tissues. Both Act-1 and CaMV35S promoters performed well in the vascular tissue of flower as opposed to the Ubi-1 promoter that did not perform in flower. |
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