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หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Effect of Sucrose and ABA on Survival Rate of Cryopreserved Protocorms of Dendrobium gratiosissimum Rchb.f.) ผู้เขียน:Sumontip Bunnag, Praweena Maneerattanarungroj, Anawat Suwanagul สื่อสิ่งพิมพ์:pdf AbstractDendrobium gratiosissimum Rchb.f. is a rare wild orchid. Cryopreservation is an alternative and important tool for ex situ preservation of orchid germplasm. In this study, protocorms of D. gratiosissimum were induced and cultured on solidified New Dogashima (ND) medium containing 0.3 mg/l NAA for two months. These protocorms were used as explants in cryopreservation using an encapsulation – dehydration method. For treatment with ABA, encapsulated protocorms were precultured in liquid ND medium containing various concentrations of ABA made up of 0, 0.01, 1 and 10 mg/l. For the sucrose treatment, beads were precultured in a semi-solid ND medium containing 0.25 M sucrose in the dark for one week at 4oC and then transferred into liquid ND medium containing 0, 0.5, 0.75 and 1 M of sucrose and then cultured for two days before dehydration. The highest percentage regrowth of cryopreserved explants was observed when precultured in the liquid ND medium supplemented with 1 mg/l ABA for one week and then dehydrated with silica gel in a laminar air-flow for five hours prior to immersion in liquid nitrogen for one hour. Regrowth ability testing performed after two weeks of treatment produced a rate of regrowth of 67% ? 0.5. Consequently, a simple and reliable protocol for the cryopreservation of D. gratiosissimum was clearly revealed in this study. |
หัวเรื่อง:ปริมาณ GA และ ABA ระหว่างการพัฒนาและการสุกแก่ของเมล็ดแตงกวา ผู้เขียน:ดร.ชวนพิศ อรุณรังสิกุล, Sutavee Sukprakarn, Shoji Shigenaga, Siriporn Chumsangchoteakul สื่อสิ่งพิมพ์:pdf AbstractThe physiological changes of Jed Bai and Puang cucumber seed were studied in rainy season during May August, 1991. Sed of both varieties reached its physiological maturity at 40 days after anthesis. The highest germination percentages of Jed Bai and Puang were obtained at 30 days and 25 days after anthesis, respectively while maximum viability was about 35 days after anthesis. Endogenous GA found in both varieties were GA1, GA3, GA4, GA7, GA19, GA20, GA24, and GA25. The highest total GA and ABA were obtained at 20 days after anthesis in both varieties but Jed Bai was higher in GA while Puang was higher in ABA than Jed Bai. First highest amount of ABA level was at 20 days after anthesis, the gradually decreased and peaked up again at 35 days after anthesis. |
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ผลงานตีพิมพ์ในวารสารวิชาการChanges in abscisic acid and antioxidant activity in sugar apples under drought conditionsผู้แต่ง:Kowitcharoen, L., Wongs-Aree, C., Setha, S., Mr.Ruangsak Komkhuntod, Srilaong, V., Kondo, S., วารสาร: |
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ผลงานตีพิมพ์ในวารสารวิชาการPre-harvest drought stress treatment improves antioxidant activity and sugar accumulation of sugar apple at harvest and during storageผู้แต่ง:Kowitcharoen, L., Wongs-Aree, C., Setha, S., Mr.Ruangsak Komkhuntod, Kondo, S., Srilaong, V., วารสาร: |
ผลงานตีพิมพ์ในวารสารวิชาการA chloroplast retrograde signal, 3'-phosphoadenosine 5'-phosphate, acts as a secondary messenger in abscisic acid signaling in stomatal closure and germinationผู้แต่ง:Dr.Wannarat Phonphoem, Assistant Professor, Gonzalo M Estavillo, Chan, K.X., Tee, E.E., Ganguly, D., Crisp, P.A., Phua, S.Y., Zhao, C., Qiu, J., Park, J., Yong, M.T., Nisar, N., Yadav, A.K., Schwessinger, B., Rathjen, J.f,, Cazzonelli, C.I., Wilson, P.B., Gilliham, M., Chen, Z.-H., Pogson, B.J., วารสาร: |
ที่มา:การประชุมเชิงปฏิบัติการ โครงการวิจัยแม่บทข้าวโพดและข้าวฟ่าง มหาวิทยาลัยเกษตรศาสตร์ ครั้งที่ 5หัวเรื่อง:การสังเคราะห์กรดแอบไซซิคในข้าวโพด (Zea mays L.) ภายใต้สภาวะเครียดน้ำและการพัฒนาเครื่องหมายดีเอนเอในการตรวจสอบยีนควบคุมการสังเคราะห์กรดแอบไซซิค |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Induction of Budbreak in Apple Trees that Received Insufficient Chilling by Hydrogen Cyanamide) ผู้เขียน:ดร.กฤษณา กฤษณพุกต์, รองศาสตราจารย์, ดร.สุรนันต์ สุภัทรพันธุ์, ศาสตราจารย์, Ryosuke Ogata สื่อสิ่งพิมพ์:pdf AbstractVarious concentrations of hydrogen cyanamide were applied to “Anna” apple to induce budbreak at the time when the trees were in dormant stage. The optimum concentration of hydrogen cyanamide to induce good budbreak was 2.5% High concentrations of hydrogen cyanamide were noticed to be toxic by dried dead shoots. ‘Tsugaru’ appli trees at different dormant stages kept in the heated glasshouse (minimum temperature was 15?C) were treated with 2.5% hydrogen cyanamide plus 50% ‘Merit’, a foliar fertilizer. It was found that the mixer of hydrogen cyanamide and ‘Merit’ could relatively induce budbreak even when buds were in the deepest dormant stage, when compared with the buds in lesser dormant stage. Buds from ‘Tsugaru’ apple trees treated or not treated with 2.5% hydrogen cyanamide were analyzed for abscisic acid (ABA) content. It was found that three days after treatment, ABA content in buds treated with hydrogen cyanamide was two times lower than that in the untreated buds. This result suggested that hydrogen cyanamide may induce budbreak by decreasing ABA content. |
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