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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Effects of ? -amylase Inhibitor on Mungbean Weevil, Callosobruchus maculatus, in vivo and in vitro and on Barley Malt ?-amylase in vitro) ผู้เขียน: อิทธิพล บรรณาการ,  ดร.ประภารัจ หอมจันทน์, ศาสตราจารย์ , นางอรุณี วงศ์ปิยะสถิตย์, ศาสตราจารย์ , Arunee Engkakul สื่อสิ่งพิมพ์:pdf AbstractEffects of protein part of ?-amylase inhibitor crude extracts from mungbean seeds of the recommended varieties, KPS1 and CN36, and mutant lines, M5-16 and M5-29 were evaluated on the mortality of the larval and adult stages of mungbean weevil, Callosobruchus maculatus. KPS1 seeds were used as the medium soaked with different extract concentrations of all mungbean varieties/lines and distilled water (the control). Each, with three replicates, was fed to one pair of C. maculatus and percent mortality of each stage was recorded. Of all different protein ?-amylase inhibitor extracts at each concentration, significant difference was not found in larval mortality percentage between the extract-treated seeds and distilled water-treated seeds. Only seeds treated with 0.2% w/w protein extracts from CN36 and KPS1 seeds significantly differed in percent adult mortality from those of the control, respectively. At 0.4, 0.6 and 1% w/w protein, each extract was significantly different from the control in adult mortality percentage. Each extract of various concentrations, except for KPS1 and M5-29 extracts gave significantly different larval mortality percentages at 0.4 and 0.6 and 1% w/w protein, respectively. There were significant differences in adult mortality percentage of every extract at each concentration from that of the control. An effect of ?-amylase inhibitor on ?-amylase activity in vitro was also studied. One hundred percent inhibition of protein part to ?-amylase activity of C. maculatus was found in all variety/line mungbean seeds. Similar study was also conducted against ? -amylase extracted from barley. The effects were less than those of ?-amylase of the weevil. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Gamma-Irradiation Induced Alterations in Mungbean ?-amylase Inhibitory Activities : Effects on ?-amylase and Development of Mungbean Weevil (Callosobruchus maculatus)) ผู้เขียน:ดร.อรุณี อิงคากุล, ผู้ช่วยศาสตราจารย์, ดร.ประภารัจ หอมจันทน์, ศาสตราจารย์, นางอรุณี วงศ์ปิยะสถิตย์, ศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractCrude ?-amylase inhibitors, the protein and the non-protein parts, were extracted from seeds of standard mungbean varieties KPS1 and CN36 and the mutant lines M5-16 and M5-29, derived from CN36 by gamma-irradiation. Only the protein part was found to produce substantial inhibition when tested against ?-amylase from adult mungbean weevil (Callosobruchus maculatus) and barley malt (Type VIIIA) with 4-5 times greater percentage inhibition by the extracts from the mutants than those from the standard varieties. In the in vivo study, feeding larval C. maculatus with seeds of KPS1 and M5-29 treated with 0.5% solution of the proteinaceous inhibitor extracted from M5-16 resulted in a significant reduction in the average number of emerging adults. The effects of the two standard varieties and the two mutant lines seeds, with and without seed coats, on two parameters related to the development of the weevil ie., the average number of eggs laid and the number of emerging adults, were also investigated. No significant differences were found in the average numbers of egg laid on seeds, with and without seed coats, of all varieties/lines. However, significant reduction in the average number of emerging adults were observed in mutant seeds, with or without seed coats, as compared with the standards. |
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