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หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Role of Modified Lysine Residues on Enzyme Kinetic of Bromoperoxidase) ผู้เขียน:Jirasak Kongkiattikajorn, นายภิญโญ พานิชพันธ์ สื่อสิ่งพิมพ์:pdf AbstractBromoperoxidase (BPO) from Thai red seaweed Gracilaria sp. has been purified and chemically modified with iodoacetamide under defined experimental conditions yielding derivatives of native bromoperoxidase. The modification increased the catalytic activity of the enzyme at concentrations of iodoacetamide up to a level of 50 mM. In order to investigate the molecular mechanism of the activation, the differences changes between native and modified enzymes were studied using kinetics methodology. The modified BPO showed greater affinity and maximal velocity for different substrates than native BPO. It has been shown that this modification reagent reacts with the ?-amino groups of the 15 out of the total 46 lysine residues of bromoperoxidase. The substrate affinity of modified BPO increased with the increases of substituents at ?-amino groups of lysine of the enzyme. The chemical modification of BPO increased its affinity for substrate monochlorodimedone (MCD) and hydrogen peroxide (about 2- and 110-fold, respectively) and also increased the maximal velocity with MCD, KBr and hydrogen peroxide (about 3-, 2.7 and 21-fold, respectively). The improvements of catalytic properties are also related to the changes in the number of ?-amino groups modified. The modification might involve neutralization of positive charges residues in BPO. The results presented in this study indicate that bromoperoxidase may acquire some new and useful characteristics related to stability and activity upon modification of specific amino acid side chains. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Effects of L-lysine on Callus Formation , Plant Regeneration and Flowering of Thai Rice c.v. KDML 105) ผู้เขียน:นายประดิษฐ์ พงศ์ทองคำ, ศาสตราจารย์เกียรติคุณ, สุรินทร์ ปิยะโชคณากุล, Prapa Sripichit, นางอมรา ทองปาน, รองศาสตราจารย์, กาญจนา กล้าแข็ง, Saowanee Ketsagul, Kanokporn Lertsirirungson สื่อสิ่งพิมพ์:pdf AbstractSeed-cultured of KDML 105 on Murashige and Skoog (MS) medium supplemented with 2 mg/ l 2,4–D , 1 g/l L-proline , 1 g/l casein hydrolysate and 20 ?M L-lysine produced the highest average percentage of callus formation (95.16%). Regeneration of the calli into plantlets could be best achieved at the average of 63.87 % in the MS medium containing 1 mg/l kinetin, 1 g/l L–proline, 300 mg/l casein hydrolysate and 20 ?M L-lysine. Plantlets were cultured in the MS medium containing 3 mg/l BAP, 3 g/l activated charcoal and 20 ?M L-lysine before transferring to pots under greenhouse condition. Two plants out of 80 plantlets reached the flowering stage under long photoperiod and produced only 6 seeds altogether. The seeds were subsequently planted for 2 generations and DNA fingerprints of these plants were determined using sequence related amplified polymorphism (SRAP) technique. All 6 clones of the L-lysine-treated plants gave different DNA patterns from that of the original KDML105 suggesting some effects of L-lysine on their genetic variation. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Requirement of Ileal Digestible Lysine for European Growing and Finishing Pigs under Tropical Conditions) ผู้เขียน:นวลจันทร์ พารักษา*, อุทัย คันโธ*, Suchaet Choenchom สื่อสิ่งพิมพ์:pdf AbstractThirty crossbred barrows averaging 24 kg body weight (BW) were used to investigate the ileal digestible lysine requirement of growing (20-40 and 40-65 kg BW) and finishing pigs (65-90 kg BW). Basal diets based on broken rice, rice bran and peanut meal were fortified with four incremental additions of L-lysine-HCl with an interval of 0.15 %. The dietary lysine contents ranging from 0.65 to 1.25 % for period 20-40 kg, 0.55 to 1.15 % for period 40-65 kg and 0.50 to 1.10 % for period 65-90 kg. Conventional control diets, containing 18.8, 16.4 and 13.8 % crude protein (CP) were also included. Daily gain, N retention , plasma urea nitrogen (PUN) during each of the 17 day balance were measured. Increasing dietary lysine content improved daily gain and protein deposition significantly (p < 0.05) for the growing periods but did not significantly affect in the finishing period. Conversely, the concentration of PUN decreased as dietary lysine concentration increased. Broken-line regression analysis determined the total dietary lysine requirements needed to optimize the daily gain, N retention and PUN as being 11.1, 16.8 and 20.0 g/d for the three stages of growth, respectively. Based on the ileal digestible lysine in feedstuffs and the total lysine contents found to optimize performance, the ileal digestible lysine requirements of European growing and finishing pigs under tropical conditions were 9.9, 15.2 and 17.0 g/d, respectively. |
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ที่มา:Agricultural Science Journal (วารสารวิทยาศาสตร์เกษตร)หัวเรื่อง:การศึกษาระดับไลซีนที่เหมาะสมสำหรับเป็ดเนื้อสายพันธ์เชอรี่วัลเลย์ภายใต้สภาพการเลี้ยงในโรงเรือนปิด |
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ผลงานตีพิมพ์ในวารสารวิชาการIntegration of Quality Protein in Waxy Maize by Means of Simple Sequence Repeat Markersผู้แต่ง:Sinkangam, B, Stamp, P, Dr.Peerasak Srinives, Professor, Dr.Peeranuch Jompuk, Associate Professor, Mongkol, W, Porniyom, A, Dang, NC, Dr.Choosak Jompuk, Associate Professor, วารสาร: |
หัวเรื่อง:ผลของไลซีนต่ออัตราการเจริญเติบโตของกุ้งกุลาลาย ผู้เขียน:นางประจวบ หลำอุบล, รองศาสตราจารย์, Bang-orn Chapoehuk สื่อสิ่งพิมพ์:pdf Abstract Using lysine 0.5, 1.0 and 1.5% in the artificial food for the treatments B, C and D while A is a control for feeding the green tiger prawn (Penaeus semisulcatus de Hann). One hundred postlarvae (P15) with an average of 1.2 centimeters body length and 0.005 gram body weight were cultured for 150 days in the concrete pond with the capacity of 2 cubicmeters (1.5 x 4.0 x 0.35 m?). The result shows that there were none significant difference in the growth rate of prawn that feeded on an artificial food with lysine an the control one. However for the fist 30 days, an average growth weight per day of D (120.66%) is considered as the best treatment and others are as C (110.00%), A (108.66%) and B (103.33%) respectively. After that the diminishing of the average growth weight was shown in all treatments. The survival rate of prawn at the end of the experiment were D (92.25%), A (88.25%) B and C (84.25%). The average production of the following treatments were D (384.93), B (335.26), A (334.70) and C 312.45 Kg/Rai/150 days respectively. |
ผลงานตีพิมพ์ในวารสารวิชาการEffect of extrusion conditions on physical and chemical properties of high protein glutinous rice-based snackผู้แต่ง:Chaiyakul, Supat, Dr.Kamolwan Jangchud, Associate Professor, Dr.Anuvat Jangchud, Associate Professor, Mr.Phaisan Wuttijumnong, Assistant Professor, Winger, Ray, วารสาร: |
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ผลงานตีพิมพ์ในวารสารวิชาการPSRQSP: An effective approach for the interpretable prediction of quorum sensing peptide using propensity score representation learningผู้แต่ง:Charoenkwan, P., Dr.Pramote Chumnanpuen, Associate Professor, Schaduangrat, N., Oh, C., Manavalan, B., Shoombuatong, W., วารสาร: |
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