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ผลงานตีพิมพ์ในวารสารวิชาการHistology and Histochemical Distribution of Goblet Cells in the Descending Colonic Epithelium of the Swamp Buffalo (Bubalus bubalis)ผู้แต่ง: Ms.Pakawadee Pongket , Mrs.Maleewan Liumsiricharoen, Associate Professor,  Supalak Romratanapan, Mr.Somchai Pongjunyakul, Associate Professor, Dr.Urai Pongchairerk, Assistant Professor, Dr.Apinun Suprasert, Professor , วารสาร: 
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การประชุมวิชาการจุลกายวิภาคศาสตร์และเนื้อเยื่อเคมีของ goblet cell ในเยื่อบุผิวลำไส้ส่วน descending colon ของกระบือปลัก (Bubalus bubalis) (2009)ผู้แต่ง:Ms.Pakawadee Pongket, Mrs.Maleewan Liumsiricharoen, Associate Professor, Mrs.Suppaluk Romratanapun, Associate Professor , Mr.Somchai Pongjunyakul, Associate Professor, Dr.Urai Pongchairerk, Assistant Professor, Dr.Apinun Suprasert, Professor, การประชุมวิชาการ: |
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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Histology and Histochemical Distribution of Goblet Cells in the Descending Colonic Epithelium of the Swamp Buffalo(Bubalus bubalis)) สื่อสิ่งพิมพ์:pdf AbstractPredominant glycoconjugates in the descending colon are synthesized and secreted by goblet cells. Changes in the chemical composition of the intestinal mucous affect the functioning of the large intestine. The expression of glycoconjugates in the colonic epithelium of buffaloes was studied using histochemical methods. Paraffin sections 3 ?m thick were stained with the following reagents: 1) haematoxylin and eosin (H&E), 2) alcian blue (AB) pH 2.5, 3) periodic acid –Schiff (PAS), 4) AB pH 2.5 – PAS, 5) high iron diamine (HID), 6) HID–AB pH 2.5, and three lectins, 7) Arachis hypogeae (PNA), 8) Ulex europaeus (UEA-I) and 9) Triticum vulgaris (WGA). Neuraminidase was used before staining with PNA. The histology of the descending colonic epithelium is characterized by an absence of villi; the epithelium have numerous simple columnar crypts. The crypts consist of columnar absorptive cells and goblet cells. The goblet cells in the descending colon of buffaloes contained acid and neutral glycoconjugates, whose expression was increased from the lower crypts to the upper crypts. There were more sulphated glycoconjugates in the goblet cells of lower crypts than in upper crypts. The crypts of the upper areas showed more intense expression of carboxylated glycoconjugates and sialic acid together with D- galactose and N- acetylglucosamine. The expression of ? -L- fucose was found in the goblet cells throughout all crypts. The results indicated that sulfomucin and ? -L- fucose played a lubricating role, while sialic acid and sialoderivatives provided protection against pathogenic infection. |
 ที่มา:การประชุมวิชาการวิทยาศาสตร์และเทคโนโลยีแห่งประเทศไทย ครั้งที่ 35 (วทท 35)หัวเรื่อง:การตรวจหาตัวรับของเชื้อไวรัสไข้หวัดใหญ่ชนิดเอในหลอดลม และปอดของสุนัขและแมวด้วยวิธี Lectin histochemical method |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Histochemical Detection of Glycoconjugates in Colonic Epithelium of the Goat) ผู้เขียน:นางสาวผกาวดี พงษ์เกษ, Suppalak Romrattanapun, นางมาลีวรรณ เหลี่ยมศิริเจริญ, รองศาสตราจารย์, ดร.ดลดา ศรีใส, อาจารย์, ดร.อภินันท์ สุประเสริฐ, ศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractThe distribution of glycoconjugates in the goat descending colon was studied by means of light microscopic histochemical methods. The staining procedures employed were horseradish peroxidase conjugated lectin, alcian blue (AB) pH 2.5, periodic acid-Schiff (PAS), ABpH 2.5-PAS, high iron diamine (HID), HID-ABpH 2.5. The lectins used in the present study were concanavalin A (ConA), Ricinus communis agglutinin I(RCA-I), Solanum tuberosum agglutinin (STL) and Limulus polyphemus agglutinin(LPA) . The result revealed that descending colonic epithelium of the goat contained acid sulfated glycoconjugates and neutral glycoconjugates with ?-D-mannose, ?-D-glucose, ?-D-galactose, Nacetylglucosamine and sialic acid residues. The quantity of neutral glycoconjugates with ?-D-mannose, ?-D-glucose, ?-D-galactose and N-acetylglucosamine increased from mucosal crypt up to mucosal surface of the epithelium, while sialic acid residues and sulfated glycoconjugates decreased from mucosal crypt to mucosal surface. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Histochemical Study of Vascular Blockage in Flower Stems of Orchids in Relation to Vase Life) ผู้เขียน:ดร.สายชล เกตุษา, ศาสตราจารย์เกียรติคุณ , Tadashi Nobuchi สื่อสิ่งพิมพ์:pdf AbstractSections were cut from the basal and upper portions above the water level of aged flower stems of Dendrobium ‘Pompadour’ and from freshly cut flower stem. Observation of cross sections by light microscope showed the presence of vascular occlusion in aged flower stems. Occlusions at the basal portion were much more than at the upper portion. Occluding material at both the basal and upper portions above the water level contained both pectin and carbohydrate, while only at the upper portion contained phenol, Lignins were not found in the occluding material. |
ที่มา:เชียงใหม่สัตวแพทยสารหัวเรื่อง:การตรวจหาเนื้อเยื่อเคมีไกลโคคอนจูเกตในต่อมนำลายกกหูของลิ่นชวา |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Lectin Histochemical Characterization of Glycoconjugates Present in Abomasal Epithelium of the Goat) ผู้เขียน:ดร.อภินันท์ สุประเสริฐ, ศาสตราจารย์, ดร.อุไร พงศ์ชัยฤกษ์, ผู้ช่วยศาสตราจารย์, นางสาวผกาวดี พงษ์เกษ, Takao Nishida สื่อสิ่งพิมพ์:pdf AbstractIn the abomasal epithelium of the goat, glycoconjugates have been studied by means of selective histochemical methods. The staining procedures employed were alcian blue (AB) pH 1.0 AB pH 2.5, Periodic acid Schiff (PAS) and AB pH 2.5-PAS. In addition twelve lectins, Triticum vulgaris WGA, Concana valin A, Glycine max SBA, Dolichos biflorus DBA, Arachis hypogea PNA, Solanum tuberosum STL, Datura stramonium DSA, Ulex europeus UEA-I, Bauhinia purpurea BPA, Ricinus communis RCAI, Lotus tetragonolobus LTA, and Limulus polyphemus LPA were applied to detect saccharides residues. According to the results obtained, the nature of glycoconjugates in mucous epithelium was found to change during cell migration from deep to superficial part. The surface mucous cells contained primarily neutral glycoconjugates with vicinal diol groupings and terminal fucose residues. By contrast, the mucous cells in deep pits and in pyloric glands contained acidic sulfated glycoconjugates with terminal sialic acid residues. The ?-D-mannose, ?-D-glucose, ?-D-galactose, N-acetylglucosamine and N-acetylgalactosamine were also found at the pyloric gland cells while they were smaller in amount at the surface mucous cells. The data suggested that changes of epithelial cells were closely correlated with cellular maturation. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Histochemical Detection of Glycoconjugates in the Anterior Lingual Salivary Glands of the Domestic Fowl) ผู้เขียน:Surapong Arthitvong, Natanun Makmee, ดร.อภินันท์ สุประเสริฐ, ศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractGlycoconjugates in secretory epithelium of the anterior lingual salivary glands of chicken have been studied by means of light microscopic histochemical methods. A series of staining procedures were employed including peroxidase - labeled lectin - diaminobenzidine methods and digestion techniques with neuraminidase or ? - amylase. The terminal portion of the anterior lingual gland consisted of mucous and seromucous cells. All the mucous cells in the glands possessed a mixture of glycoconjugates of different nature, but they could be divided into three types, on the basis of the principle type of glycoconjugates involved, namely primarily sulfated, carboxylated and neutral glycoconjugate-containing cells. The first mentioned type of cells was predominant. The presence of sialic acid, ? - D - mannose, ? - D - glucose and ? - D - galactose in these glycoconjugates was confirmed. The seromucous cells found in the anterior lingual gland contained a very small amount of comparable carbohydrates predominantly in the apical cytoplasm. Possible functional activities of the glands were discussed. |
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