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 การประชุมวิชาการจุลกายวิภาคศาสตร์และเนื้อเยื่อเคมีของ goblet cell ในเยื่อบุผิวลำไส้ส่วน descending colon ของกระบือปลัก (Bubalus bubalis) (2009)ผู้แต่ง: Ms.Pakawadee Pongket , Mrs.Maleewan Liumsiricharoen, Associate Professor, Mrs.Suppaluk Romratanapun, Associate Professor , Mr.Somchai Pongjunyakul, Associate Professor, Dr.Urai Pongchairerk, Assistant Professor, Dr.Apinun Suprasert, Professor , การประชุมวิชาการ: 
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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Histology and Histochemical Distribution of Goblet Cells in the Descending Colonic Epithelium of the Swamp Buffalo(Bubalus bubalis)) สื่อสิ่งพิมพ์:pdf AbstractPredominant glycoconjugates in the descending colon are synthesized and secreted by goblet cells. Changes in the chemical composition of the intestinal mucous affect the functioning of the large intestine. The expression of glycoconjugates in the colonic epithelium of buffaloes was studied using histochemical methods. Paraffin sections 3 ?m thick were stained with the following reagents: 1) haematoxylin and eosin (H&E), 2) alcian blue (AB) pH 2.5, 3) periodic acid –Schiff (PAS), 4) AB pH 2.5 – PAS, 5) high iron diamine (HID), 6) HID–AB pH 2.5, and three lectins, 7) Arachis hypogeae (PNA), 8) Ulex europaeus (UEA-I) and 9) Triticum vulgaris (WGA). Neuraminidase was used before staining with PNA. The histology of the descending colonic epithelium is characterized by an absence of villi; the epithelium have numerous simple columnar crypts. The crypts consist of columnar absorptive cells and goblet cells. The goblet cells in the descending colon of buffaloes contained acid and neutral glycoconjugates, whose expression was increased from the lower crypts to the upper crypts. There were more sulphated glycoconjugates in the goblet cells of lower crypts than in upper crypts. The crypts of the upper areas showed more intense expression of carboxylated glycoconjugates and sialic acid together with D- galactose and N- acetylglucosamine. The expression of ? -L- fucose was found in the goblet cells throughout all crypts. The results indicated that sulfomucin and ? -L- fucose played a lubricating role, while sialic acid and sialoderivatives provided protection against pathogenic infection. |
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ที่มา:การประชุมวิชาการวิทยาศาสตร์และเทคโนโลยีแห่งประเทศไทย ครั้งที่ 35 (วทท 35)หัวเรื่อง:การตรวจหาตัวรับของเชื้อไวรัสไข้หวัดใหญ่ชนิดเอในหลอดลม และปอดของสุนัขและแมวด้วยวิธี Lectin histochemical method |
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หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Histochemical Characterization of Glycoconjugates Present in Cloacal Epithelium of the Chicken) ผู้เขียน:ดร.อภินันท์ สุประเสริฐ, ศาสตราจารย์, นายบุญธรรม จงเจริญ, รองศาสตราจารย์,  Suppaluk Romrattanapun สื่อสิ่งพิมพ์:pdf AbstractThe distribution of glycoconjugates in the chicken cloaca was studied by means of light microscopic histochemical methods. The staining procedures employed were horseradish peroxidase conjugated lectin, Alcian blue (AB) pH 1.0, AB pH 2.5, periodic acid –Schiff (PAS), AB pH 2.5-PAS in combination with enzyme neuraminidase digestion. The lectin used in the present study were peanut agglutinin (PNA), wheat germ agglutinin (WGA), Dolichos biflorus agglutinin (DBA), and Lotus tetragonolobus agglutinin (LTA) The mucosa of both coprodeum and urodeum is covered by simple columnar epithelium in which 2 type of cells are encountered-columnar and goblet cells. In contrast, one layer of only columna cells is found in the mucosa of proctodeum. Tubular glands of proctodeum are commonly provided at submucosal layer. All the goblet cells in coprodeum and urodeum and tubular glands of proctodeum were coloured blue and purple with AB pH 1.0 and AB pH 2.5-PAS, respectively. They were furthermore stained moderately with WGA. Neuraminidase digestion changed the blue coloration with AB pH 2.5 to pale blue. Removal of sialic acid with neuraminidase imparted weak to strong affinity of PNA. Histophysiological significances of glycoconjugates involved in the chicken cloaca were discussed with special reference to the functional activities of the carbohydrates. |
ที่มา:เชียงใหม่สัตวแพทยสารหัวเรื่อง:การตรวจหาเนื้อเยื่อเคมีไกลโคคอนจูเกตในต่อมนำลายกกหูของลิ่นชวา |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Histochemical Study of Vascular Blockage in Flower Stems of Orchids in Relation to Vase Life) ผู้เขียน:ดร.สายชล เกตุษา, ศาสตราจารย์ , Tadashi Nobuchi สื่อสิ่งพิมพ์:pdf AbstractSections were cut from the basal and upper portions above the water level of aged flower stems of Dendrobium ‘Pompadour’ and from freshly cut flower stem. Observation of cross sections by light microscope showed the presence of vascular occlusion in aged flower stems. Occlusions at the basal portion were much more than at the upper portion. Occluding material at both the basal and upper portions above the water level contained both pectin and carbohydrate, while only at the upper portion contained phenol, Lignins were not found in the occluding material. |
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หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Histochemical Detection of Glycoconjuagates in Chicken Colonic Epithelium with a Specific Horseradish Peroxidase Conjuagated Lectin) ผู้เขียน:ดร.อภินันท์ สุประเสริฐ, ศาสตราจารย์, นายบุญธรรม จงเจริญ, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractChicken colons were stained with a battery of seven horseradish peroxidase-conjugated lectins : Ricnus communis agglutinin-I (RCA-I) peanut agglutinin, wheat germ agglutinin, Ulex europaeus agglutinin, Limax fiavus agglutinin, Dolichos biflorus agglutinin, and Pisum sativum agglutinin. Lectin staining revealed striking differences in the structure of oligosaccharides between intracellular secretory glycoconjugates in goblet cells and in columnar cells. The mucous granules of goblet cells were found to contain glycoconjugates with sialic acid-galactose dimer,D-mannose,D-glucose, N-acety-D-glucosamine and D-galactose residues. In contrast, terminal or internal N-acety-D-glucosamine and terminal galactose-N (1-3)-acety-D-galactosamine disaccharides were detected in supranuclear region of the columner cells. The striated border of the colonic columnar cells were also found to contain glycoconjugates similar in reactivity to those of the goblet cells. The existence of glycoconjugates with various saccharide residues in colonic epithelium of the chicken is reported for the first time in this study. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Histochemical Detection of Glycoconjugates in Colonic Epithelium of the Goat) ผู้เขียน:นางสาวผกาวดี พงษ์เกษ, Suppalak Romrattanapun, นางมาลีวรรณ เหลี่ยมศิริเจริญ, รองศาสตราจารย์, ดร.ดลดา ศรีใส, อาจารย์, ดร.อภินันท์ สุประเสริฐ, ศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractThe distribution of glycoconjugates in the goat descending colon was studied by means of light microscopic histochemical methods. The staining procedures employed were horseradish peroxidase conjugated lectin, alcian blue (AB) pH 2.5, periodic acid-Schiff (PAS), ABpH 2.5-PAS, high iron diamine (HID), HID-ABpH 2.5. The lectins used in the present study were concanavalin A (ConA), Ricinus communis agglutinin I(RCA-I), Solanum tuberosum agglutinin (STL) and Limulus polyphemus agglutinin(LPA) . The result revealed that descending colonic epithelium of the goat contained acid sulfated glycoconjugates and neutral glycoconjugates with ?-D-mannose, ?-D-glucose, ?-D-galactose, Nacetylglucosamine and sialic acid residues. The quantity of neutral glycoconjugates with ?-D-mannose, ?-D-glucose, ?-D-galactose and N-acetylglucosamine increased from mucosal crypt up to mucosal surface of the epithelium, while sialic acid residues and sulfated glycoconjugates decreased from mucosal crypt to mucosal surface. |