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ผลงานตีพิมพ์ในวารสารวิชาการDeveloping DNA markers for assisting selection of field weathering resistance in soybeanผู้แต่ง: Ye, C.,  Dr.Prapa Sripichitt, Associate Professor , Dr.Vipa Hongtrakul, Associate Professor , Mrs.Sunanta Juntakool, Assistant Professor , Sripichitt, A., Fukai, S., วารสาร: |
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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Modifying Controlled Deterioration for Evaluating Field Weathering Resistance of Soybean) ผู้เขียน:ดร.ประภา ศรีพิจิตต์, รองศาสตราจารย์, นางสุนันทา จันทกูล, ผู้ช่วยศาสตราจารย์, ดร.วิภา หงษ์ตระกูล, รองศาสตราจารย์, Arom Sripichitt สื่อสิ่งพิมพ์:pdf AbstractTo develop practical methods for testing field weathering resistance of soybean varieties, pods and seeds from CM60 (susceptible) and GC10981 (resistant) were tested by seven treatments. Among the treatments, modified incubator weathering (yellow pods were incubated at 30?C under 90-100% relative humidity for 7 days) and the controlled deterioration (dry seeds were soaked in distilled water for 60 minutes and then incubated at 41?C under 90-100% relative humidity for 3 days) showed widerange differences in seed germination and viability between CM60 and GC10981. These two treatments were then tested on 11 soybean varieties comparing with a field weathering treatment. The germination of seeds treated by controlled deterioration was highly correlated to the germination of seeds subjected to field weathering treatment (r=0.964**, n=11). The viability of seeds submitted to both incubator weathering and controlled deterioration were also correlated to the viability of seeds exposed to field weathering (r=0.697* and 0.716*, n=11). The modified incubator weathering and controlled deterioration methods were further used to evaluate the field weathering resistance of 139 F2 progenies derived from the cross CM60/GC10981. There was a significant correlation between the incubator weathering and the controlled deterioration by considering the germination and viability of seeds (germination r=0.331**, viability r=0.425**, n=139). Both the modified incubator weathering and controlled deterioration were efficient for evaluating the field weathering resistance of soybean varieties. Particularly, controlled deterioration method was found to be a useful way for evaluating the field weathering resistance of soybean seeds. |
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หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Evaluation of Soybean [Glycine max (L.) Merrill] Germplasm for Field Weathering Resistance using Seed Quality and SCAR Markers) ผู้เขียน:Nan Pa Pa Win, ดร.ประภา ศรีพิจิตต์, รองศาสตราจารย์, ดร.วันชัย จันทร์ประเสริฐ, รองศาสตราจารย์ , ดร.วิภา หงษ์ตระกูล, รองศาสตราจารย์, เฉลิมพล ภูมิไชย์ สื่อสิ่งพิมพ์:pdf AbstractForty-eight soybean varieties/lines along with resistance and susceptibility checks were grown at the National Corn and Sorghum Research Center, Nakhon Ratchasima province, Thailand in a randomized complete block design with three replications. At physiological maturity, soybean pods were harvested and subjected to incubator weathering, controlled deterioration and electrical conductivity tests to evaluate the seed quality. Soybean seed physical characteristics, including seed coat percentage, seed weight and seed coat color were also investigated. Two sequence characterized amplified region (SCAR) primers, Eaag/Mcac-233 and Eact/Mctt-157, previously reported to link with a quantitative trait locus (QTL) that controlled field weathering resistance, were used to amplify the DNA of the 50 soybean varieties/lines. The results revealed that 48 soybean varieties/lines were significantly different in seed quality and seed physical characteristics. They were classified into four groups: resistant, moderately resistant, moderately susceptible and susceptible to field weathering, according to the average germination percentage after incubator weathering and controlled deterioration. The overall field weathering resistance was found to correlate with seed quality and seed physical characteristics. The two highest correlation coefficients were found between field weathering resistance and germination percentage after incubator weathering (r = 0.898**) and controlled deterioration (r = 0.888**). The two SCAR primers were able to differentiate between resistance and susceptibility checks but were unable to classify field weathering resistance in 48 soybean varieties/lines. It was concluded that the two markers were not appropriate for evaluating field weathering resistance in the soybean varieties/lines. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Inheritance of Field Weathering Resistance in Soybean [Glycine max (L.) Merrill]) ผู้เขียน:Myint, ดร.ประภา ศรีพิจิตต์, รองศาสตราจารย์, ดร.วันชัย จันทร์ประเสริฐ, รองศาสตราจารย์, ดร.วิภา หงษ์ตระกูล, รองศาสตราจารย์, Arom Sripichitt สื่อสิ่งพิมพ์:pdf AbstractA commercial variety of soybean, Chiangmai 60, which is susceptible to field weathering and two field weathering resistant varieties, GC 10848 and Kalitur, were grown and hybridized in the greenhouse at the Department of Agronomy, Kasetsart University. The F1 hybrid seeds and their parental varieties were planted in the greenhouse to produce F2 seeds. Parental varieties, F1 hybrids and F2 progenies were grown in an experimental field during the 2008 dry season at the National Corn and Sorghum Research Center, Pakchong District, Nakhon Ratchasima province. Individual plots were 3?3 m2, with six rows, composed of two rows of parents and four rows of F1 hybrids or F2 progenies for each cross. The spacing between rows was 50 cm and between hills was 25 cm. At physiological maturity, soybean pods were harvested, threshed and subjected to tests for accelerated aging (AA) and electrical conductivity (EC), and the seed coat percentage was measured. Field weathering resistance of the parental plants, F1 hybrids and F2 progenies was evaluated using germination percentages after AA testing and EC values of seed leachate and seed coat percentages. Dominance percentages of F1 hybrids and the frequency distribution of F2 progenies for the germination percentages after AA testing and EC values of seed leachate and seed coat percentages of the two soybean crosses revealed that field weathering resistance was controlled by a polygene with partial dominance. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Breeding of Soybean (Glycine max (L.) Merrill) for Field Weathering Resistance by Pedigree Method) ผู้เขียน:Phan THI THANH, ดร.ประภา ศรีพิจิตต์, รองศาสตราจารย์, ดร.วาสนา วงษ์ใหญ่, รองศาสตราจารย์, นางสุนันทา จันทกูล, ผู้ช่วยศาสตราจารย์, Arom Sripichitt สื่อสิ่งพิมพ์:pdf AbstractBreeding of soybean for field weathering resistance was conducted by hybridization between commercial variety CM60 which is susceptible to field weathering and two field weathering resistant varieties (GC2796 and SJ1) in 2003. The F1 hybrid plants were grown in the greenhouse at the Department of Agronomy, Kasetsart University and the F2 to F4 progenies were planted in the field at National Corn and Sorghum Research Center, Nakhon Ratchasima province. Field weathering resistance of soybean was evaluated from some seed characteristics involving seed germinability after accelerated aging, seed vigor estimated from the electrical conductivity of seed leachates, percentage of seed coat and seed coat thickness. Selection was done in each succeeding generation from F2 to F4 using pedigree method for plants/lines which had high seed germinability and vigor, high percentage and thickness of seed coat and good agronomic characters. The yield trial of 17 F5 lines was performed at National Corn and Sorghum Research Center in rainy season 2005. Six F5 lines having high seed yield, field weathering resistance and good agronomic characters were selected. These promising lines will be further evaluated for seed yield, field weathering resistance and agronomic performance in different locations. |
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