Search Result of "antibody detection"

About 12 results
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ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Indirect Enzyme-Linked Immunosorbent Assay Test Kit Development for Specific Antibody Detection Against Brucella abortus in Cattle)

ผู้เขียน:ImgMonaya Ekgatat, ImgSuree Thammasart, ImgReka Kanitpun, ImgSurapong Wongkasemjit, ImgChokchai Nokdhes, ImgUtit Trenuntawan

สื่อสิ่งพิมพ์:pdf

Abstract

The smooth lipopolysaccharide (SLPS) antigen, prepared from Brucella abortus strain 99 was used in an indirect Enzyme-Linked Immunosorbent Assay (iELISA). The data were expressed as optical density and generated using computer software. The positive results were accepted at the cut-off values of ? 40 percent positivity (PP). The strong positive serum control (C++), weak positive serum control (C+), negative serum control (C-), and conjugate control (Cc) were used to optimize the test kit. The iELISA was optimization and standardization for antigen concentration, test material dilution and detection system dilution by checkerboard titration. The statistic evaluation using data from sera from 317 infected and 5,300 non-infected cattle revealed that the sensitivity (Se) is 99.369 (98.497-100.0)%, specificity (Sp) is 99.887 (99.796-99.977)%, accuracy (Ac) is 99.858% and Kappa value is 0.987. The iELISA test kit was subsequently validated by testing 9,877 field serum samples submitted to laboratory. The complement fixation test was used as gold standard. The results revealed that the relative Se is 99.026 (97.929-100.0)%, relative Sp is 98.547 (98.308-98.787)%, relative Ac is 98.562% and Kappa value is 0.804 when cattle sera were tested. The components of this iELISA test kit consisted of antigen, control sera, and essential diluents for 800 tests. This test showed high sensitivity, specificity, and accuracy and could be performed in mass. Therefore, this iELISA test kit might be an appropriate tool for brucellosis control in Thailand.

Article Info
Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 042, Issue 5, Dec 99 - Dec 99, Page 95 - 100 |  PDF |  Page 

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ที่มา:วารสาร คณะสัตวแพทยศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Comparison of Serological Tests for Antibody Detection Against Brucella melitensis Infection in Goats)

ผู้เขียน:ImgMonaya Ekgatat, ImgReka Kanitpun, ImgPittaya Kunchit, ImgWorawan Arampong, ImgSornsak Raksajit, ImgSuree Thammasart, ImgUtit Trenuntawan, ImgPhairuch Tumcha, ImgSurapong Wongkasemjit

สื่อสิ่งพิมพ์:pdf

Abstract

Four serological assays, including Rose Bengal test (RBT), modified RBT (mRBT), complement fixation test (CET) and indirect enzymelinked immunosorbent assay (I-ELISA), were evaluated for their efficiency on diagnosis of brucellosis in goats. Serum to antigen ratio, used for RBT and MRBT, which allowed for optimal sensitivity, was 1:1 and 3:1, respectively. CET was performed using microtechnique combined with cold fixation protocol. I-ELISA was performed using conjugated protein G and smooth lipopolysaccharide (sLPS) as antigen with percent positivity (PP) cutoff at 30. Specimens tested included 1,317 goat sera, of which 127 were positive and 1,190 were negative as confirmed by isolation of Brucella melitensis. At 95% of confidence, diagnostic sensitivity (DSe) was 99.2% by RBT,100 % by mRBT, 95.3%by CFT and 99.2% by I-ELISA, while their diagnostic specificity (DSp) was 100% by RBT, mRBT and CFT , and 99.9% by I-ELISA,Comparison of each assay with B. melitensis isolation showed that accuracy (Ac) , kappa (K) value , positive predictive value (PPV) and negative predictive value (NPV) were high. The results of this study showed that RBT, mRBT and I-ELISA were sensitive, specific and suitable for screening , while CFT was suitable for confirmation of Brucella spp. infection in goats. Further study on herd prevalence of brucellosis is needed for the dvelopment of a long-term national prevention and control program of brucellosis in goats.

Article Info
วารสาร คณะสัตวแพทยศาสตร์(Journal of Kasetsart Veterinarians), Volume 020, Issue 1, Jan 10 - Apr 10, Page 19 - 26 |  PDF |  Page 

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ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Detection of Salmonella in Food Samples by Dot-ELISA using Polyclonal Antibody)

ผู้เขียน:Imgกฤษฎา ตู้จินดา, ImgBoontharika Naknam, Imgพัชรี สุนทรนันท, ImgChaiwat Kittikul, Imgดร.องอาจ เลาหวินิจ, รองศาสตราจารย์

สื่อสิ่งพิมพ์:pdf

Abstract

The immunological method of dot-ELISA(enzyme linked immunosorbent assay) was performed using a polyclonal antibody produced in rabbits immunized with Salmonella ser. Anatum and Salmonella ser. Enteritidis. The purification of immunoglobulin G (IgG) was carried out by affinity chromatography and the purity was characterized by SDS-PAGE. The purified IgG was used to detect Salmonella by the dot-ELISA method. It was found that all Salmonella serovars representative of each group were positive. However, there was cross reaction with other enteric Gram-negative bacteria. Therefore, Rappaport Vassiliadis Soya (RVS) selective enrichment broth was used to select and increase the number of Salmonella (but inhibit the other enteric Gram-negative bacteria) which then were examined by the dot- ELISA method. It was found that this method was able eliminate the cross reaction as well. Detection of Salmonella from food samples using RVS broth followed by the dot-ELISA method, compared with a modified standard method (ISO 6579:2002) was performed. Forty-eight out of 175 food samples (27.43%) were positive for salmonellae detection which was the same result as by the standard method. Detection using RVS broth followed by the dot-ELISA method took only 24 h, which was shorter than the standard method. Moreover, determination by the dot-ELISA method was easy and used lower amounts of reagents, as well as being cheaper and highly sensitive.

Article Info
Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 045, Issue 3, May 11 - Jun 11, Page 444 - 450 |  PDF |  Page 

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ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Detection of Antibodies Against Melioidosis from Animal Sera in Thailand by Indirect Haemagglutination Test)

ผู้เขียน:Imgนิตยา ศรีแก้วเขียว, Imgดร.องอาจ เลาหวินิจ, รองศาสตราจารย์

สื่อสิ่งพิมพ์:pdf

Abstract

Antibody detection against melioidosis from 8,153 serum samples of cattle, sheep, goats, pigs and deers of 18 provinces in Thailand was done by using indirect haemagglutination test during the year 2005-2006 (6,576 goat sera , 1,050 pig sera, 78 cattle sera , 83 deer sera and 366 sheep sera). Serum antibodies titer at ? 1:160 against melioidosis were found in goats (0.33%), pigs (7.23%), cattle (2.56%), deers (7.23%) and sheep (6.83%), however, more information is needed for the conclusion of this study.

Article Info
Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 041, Issue 5, Jan 07 - Dec 07, Page 81 - 85 |  PDF |  Page 

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ที่มา:การประชุมวิชาการอารักขาพืชแห่งชาติ ครั้งที่ 9 "อารักขาพืชไทย เทิดไท้องค์ภูมี ตามวิถีเศรษฐกิจพอเพียง"

หัวเรื่อง:การพัฒนาวิธีการตรวจสอบ Ochratoxin A โดยใช้โมโนโคลนอลแอนติบอดี

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ผลงานตีพิมพ์ในวารสารวิชาการ

Detection of Antibodies Against Melioidosis from Animal Sera in Thailand by Indirect Haemagglutination Test

ผู้แต่ง:ImgNittaya Srikawkheaw, ImgDr.Ong-ard Lawhavinit, Associate Professor,

วารสาร:

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ผลงานตีพิมพ์ในวารสารวิชาการ

Multispecies detection of antibodies to influenza A viruses by a double-antigen sandwich ELISA

ผู้แต่ง:ImgWatcharatanyatip, K, ImgBoonmoh, S, ImgChaichoun, K, ImgDr.Thaweesak Songserm, Professor, ImgWoratanti, M, ImgDharakul, T,

วารสาร:

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Researcher

ดร. ทวีศักดิ์ ส่งเสริม, ศาสตราจารย์

ที่ทำงาน:ภาควิชาพยาธิวิทยา คณะสัตวแพทยศาสตร์ กำแพงแสน

สาขาที่สนใจ:Avian Pathology, Immunology, Diagnostic Pathology

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Researcher

ดร. องอาจ เลาหวินิจ, รองศาสตราจารย์

ที่ทำงาน:ภาควิชาจุลชีววิทยาและวิทยาภูมิคุ้มกัน คณะสัตวแพทยศาสตร์ บางเขน

สาขาที่สนใจ:จุลชีววิทยา, ภูมิคุ้มกันโรคสัตว์น้ำ, โรคเชื้อโรคก่อโรคในสัตว์, การเพาะเลี้ยงและป้องกันโรคสัตว์น้ำ

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